Genetic characteristics and phylogeography of the habitat generalist mayfly Ecdyonurus yoshidae (Ephemeroptera: Heptageniidae) in the Japanese archipelago

Hiroaki Kaneko, Shin ichi Ishiwata, Yeon Jae Bae, Takeji Takamura-Enya

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Because of their early divergence in insect evolution, unique aquatic larval stage, and low dispersal ability, many mayfly species exhibit genetic structures that accurately reflect geological history and paleoclimatic changes. The present study investigated the genetic characteristics of Ecdyonurus yoshidae Takahashi (Ephemeroptera: Heptageniidae), a mayfly that inhabits a variety of freshwater habitats in Japan. Mitochondrial cytochrome c oxidase subunit I (COI) and 16S ribosomal RNA (16S rRNA) gene sequences were generated from 209 mayfly specimens that were collected from 109 sites in Japan. A total of 145 haplotypes were detected, and nine of the lineages exhibited geographical regionality, with one lineage being endemic to Lake Biwa. The haplotype network was “bottleneck type” and included both star-like structures and missing (or unobserved) haplotypes. Together, the haplotype network and FST values indicated that the Fukuejima Island group (Clade I) was genetically distinct from groups on the island of Kyushu, which is separated from Fukuejima Island by the Gotonada Sea. The common ancestor of E. yoshidae lineages in Japan was estimated to have diverged 1.14 mega-annum (Ma) (95% highest posterior density interval, 0.55–1.78 Ma), and more regional genetic diversities were generated during the Middle to Late Pleistocene. Genetically distinct lineages of eastern and western Japan were separated by the Kinki Triangle region in central Japan. The genetic diversity of the habitat generalist E. yoshidae increased during the Middle and Late Pleistocene, when crustal movement and climate change in the Japanese archipelago occurred simultaneously.

Original languageEnglish
Pages (from-to)238-250
Number of pages13
JournalEntomological Research
Volume51
Issue number5
DOIs
Publication statusPublished - 2021 May

Bibliographical note

Funding Information:
We would like to thank Drs. R. B. Kuranishi (Kanagawa Institute of Technology), K. Tojo (Shinshu University), S. Osozawa (KawaOso Molecular Bio-Geology Institute), M. Nishino (Former Biwako Seikei Sport College), A. Nozaki (Hiratsuka City Museum), T. M. Tiunova (Federal Scientific Center of the East Asia Terrestrial Biodiversity Far Eastern Branch of Russian Academy of Sciences), K. Tominaga (Kwansei Gakuin Senior High School), T. Suzuki (Shinshu University), and Y. Iwasaki (National Institute of Advanced Industrial Science and Technology) for their useful advice regarding our study. We would also like to thank Drs. E. Inoue (Lake Biwa Environmental Research Institute), T. Ishihara (Natural Museum “Mori-no-fureai Hall”), K. Ishikawa (Lake Biwa Environmental Research Institute), S. Kanada (Former Nihon Kogakuin College), S. Kobayashi (Wenzhou University), Y. Sakai (Lake Biwa Environmental Research Institute), M. Sakata (Okayama Prefectural Nature Conservation Center), N. Shimura (Yokohama City Kanagawa Prefecture), T. Tanaka (Fisheries Research Institute, Aomori Prefectural Industrial Technology Research Center), K. Tomikawa (Hiroshima University), D. Tsuruda (Hino City Tokyo), and M. Uenishi (Ryukoku University) for providing information and specimens in this study.

Publisher Copyright:
© 2021 The Entomological Society of Korea and John Wiley & Sons Australia, Ltd

Keywords

  • 16S rRNA
  • COI
  • Ecdyonurus yoshidae
  • Japanese archipelago
  • demographic history
  • mayfly

ASJC Scopus subject areas

  • Insect Science

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