Genetic diversity within the Albugo candida complex (Peronosporales, Oomycota) inferred from phylogenetic analysis of ITS rDNA and COX2 mtDNA sequences

Young Joon Choi; Seung Beom Hong; Hyeon Dong Shin

doi:10.1016/j.ympev.2006.03.023

Genetic diversity within the Albugo candida complex (Peronosporales, Oomycota) inferred from phylogenetic analysis of ITS rDNA and COX2 mtDNA sequences

Young Joon Choi, Seung Beom Hong, Hyeon Dong Shin

Research output: Contribution to journal › Article › peer-review

51 Citations (Scopus)

Abstract

Albugo candida is a destructive fungus infecting brassicaceous hosts. The genetic diversity within the A. candida complex from various host plants was investigated by sequence analysis of the internal transcribed spacer (ITS) region of rDNA and the cytochrome c oxidase subunit II (COX2) region of mtDNA. The aligned nucleotide sequences of A. candida shared significantly high distances, up to 20.4 and 8.9%, in two genes. The phylogenetic trees, obtained using the Bayesian method and maximum parsimony analysis, showed two separate groups that corresponded to the host genera. Group I included A. candida isolates infecting Arabis, Autrieta, Berteroa, Biscutella, Brassica, Cardaminopsis, Diplotaxis, Eruca, Erysimum, Heliophila, Iberis, Lunaria, Raphanus, Sinapis, Sisymbrium, and Thlaspi. Group II contained all isolates from Capsella, Descurainia, Diptychocarpus, Draba, and Lepidium. The genetic similarities between the two genes among isolates within Group I were 99.0-100% and 99.6-100%, while those within Group II were 90.4-100% and 91.1-100%, respectively, showing considerably lower values than for Group I. The A. candida isolates from Capsella bursa-pastoris in Korea are clearly separated by sequence analysis for the two genes compared to those from Wales, England, and the USA. Based on the molecular data from the two genes, we suggest the high degree of genetic diversity exhibited within A. candida complexes warrants their division into several distinct species.

Original language	English
Pages (from-to)	400-409
Number of pages	10
Journal	Molecular Phylogenetics and Evolution
Volume	40
Issue number	2
DOIs	https://doi.org/10.1016/j.ympev.2006.03.023
Publication status	Published - 2006 Aug

Bibliographical note

Funding Information:
The authors are grateful to Micheal Priest, the curator of Plant Pathology herbarium (DAR), for helpful comments and suggestion, to the curators of BPI (National Fungus Collections, Beltsville, Maryland, USA), CUP (Plant Pathology Herbarium, Cornell University, New York, USA), and SOMF (Mycological Collection of Institute of Botany, Bulgarian Academy of Sciences, Sofia, Bulgaria) for providing the A. candida specimens, and to staff at KACC (Korean Agricultural Culture Collection) for their technical assistance in the DNA sequencing and phylogenetic analysis. This work was financially supported by a research grant from the Korea Research Foundation (KRF-2003-015-C00611).

Keywords

Albugo
Brassicaceae
COX2 mtDNA
ITS rDNA
Species complex

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics
Molecular Biology
Genetics

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10.1016/j.ympev.2006.03.023

Cite this

@article{be7348e7336343529b45dea6de13c657,

title = "Genetic diversity within the Albugo candida complex (Peronosporales, Oomycota) inferred from phylogenetic analysis of ITS rDNA and COX2 mtDNA sequences",

abstract = "Albugo candida is a destructive fungus infecting brassicaceous hosts. The genetic diversity within the A. candida complex from various host plants was investigated by sequence analysis of the internal transcribed spacer (ITS) region of rDNA and the cytochrome c oxidase subunit II (COX2) region of mtDNA. The aligned nucleotide sequences of A. candida shared significantly high distances, up to 20.4 and 8.9%, in two genes. The phylogenetic trees, obtained using the Bayesian method and maximum parsimony analysis, showed two separate groups that corresponded to the host genera. Group I included A. candida isolates infecting Arabis, Autrieta, Berteroa, Biscutella, Brassica, Cardaminopsis, Diplotaxis, Eruca, Erysimum, Heliophila, Iberis, Lunaria, Raphanus, Sinapis, Sisymbrium, and Thlaspi. Group II contained all isolates from Capsella, Descurainia, Diptychocarpus, Draba, and Lepidium. The genetic similarities between the two genes among isolates within Group I were 99.0-100% and 99.6-100%, while those within Group II were 90.4-100% and 91.1-100%, respectively, showing considerably lower values than for Group I. The A. candida isolates from Capsella bursa-pastoris in Korea are clearly separated by sequence analysis for the two genes compared to those from Wales, England, and the USA. Based on the molecular data from the two genes, we suggest the high degree of genetic diversity exhibited within A. candida complexes warrants their division into several distinct species.",

keywords = "Albugo, Brassicaceae, COX2 mtDNA, ITS rDNA, Species complex",

author = "Choi, {Young Joon} and Hong, {Seung Beom} and Shin, {Hyeon Dong}",

note = "Funding Information: The authors are grateful to Micheal Priest, the curator of Plant Pathology herbarium (DAR), for helpful comments and suggestion, to the curators of BPI (National Fungus Collections, Beltsville, Maryland, USA), CUP (Plant Pathology Herbarium, Cornell University, New York, USA), and SOMF (Mycological Collection of Institute of Botany, Bulgarian Academy of Sciences, Sofia, Bulgaria) for providing the A. candida specimens, and to staff at KACC (Korean Agricultural Culture Collection) for their technical assistance in the DNA sequencing and phylogenetic analysis. This work was financially supported by a research grant from the Korea Research Foundation (KRF-2003-015-C00611).",

year = "2006",

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doi = "10.1016/j.ympev.2006.03.023",

language = "English",

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journal = "Molecular Phylogenetics and Evolution",

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AU - Hong, Seung Beom

AU - Shin, Hyeon Dong

N1 - Funding Information: The authors are grateful to Micheal Priest, the curator of Plant Pathology herbarium (DAR), for helpful comments and suggestion, to the curators of BPI (National Fungus Collections, Beltsville, Maryland, USA), CUP (Plant Pathology Herbarium, Cornell University, New York, USA), and SOMF (Mycological Collection of Institute of Botany, Bulgarian Academy of Sciences, Sofia, Bulgaria) for providing the A. candida specimens, and to staff at KACC (Korean Agricultural Culture Collection) for their technical assistance in the DNA sequencing and phylogenetic analysis. This work was financially supported by a research grant from the Korea Research Foundation (KRF-2003-015-C00611).

PY - 2006/8

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N2 - Albugo candida is a destructive fungus infecting brassicaceous hosts. The genetic diversity within the A. candida complex from various host plants was investigated by sequence analysis of the internal transcribed spacer (ITS) region of rDNA and the cytochrome c oxidase subunit II (COX2) region of mtDNA. The aligned nucleotide sequences of A. candida shared significantly high distances, up to 20.4 and 8.9%, in two genes. The phylogenetic trees, obtained using the Bayesian method and maximum parsimony analysis, showed two separate groups that corresponded to the host genera. Group I included A. candida isolates infecting Arabis, Autrieta, Berteroa, Biscutella, Brassica, Cardaminopsis, Diplotaxis, Eruca, Erysimum, Heliophila, Iberis, Lunaria, Raphanus, Sinapis, Sisymbrium, and Thlaspi. Group II contained all isolates from Capsella, Descurainia, Diptychocarpus, Draba, and Lepidium. The genetic similarities between the two genes among isolates within Group I were 99.0-100% and 99.6-100%, while those within Group II were 90.4-100% and 91.1-100%, respectively, showing considerably lower values than for Group I. The A. candida isolates from Capsella bursa-pastoris in Korea are clearly separated by sequence analysis for the two genes compared to those from Wales, England, and the USA. Based on the molecular data from the two genes, we suggest the high degree of genetic diversity exhibited within A. candida complexes warrants their division into several distinct species.

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ER -

Genetic diversity within the Albugo candida complex (Peronosporales, Oomycota) inferred from phylogenetic analysis of ITS rDNA and COX2 mtDNA sequences

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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