TY - JOUR
T1 - Genetic framework for flowering-time regulation by ambient temperature-responsive miRNAs in Arabidopsis
AU - Lee, Hanna
AU - Yoo, Seong Jeon
AU - Lee, Jeong Hwan
AU - Kim, Wanhui
AU - Yoo, Seung Kwan
AU - Fitzgerald, Heather
AU - Carrington, James C.
AU - Ahn, Ji Hoon
PY - 2010/1/27
Y1 - 2010/1/27
N2 - Flowering is the primary trait affected by ambient temperature changes. Plant microRNAs (miRNAs) are small non-coding RNAs playing an important regulatory role in plant development. In this study, to elucidate the mechanism of flowering-time regulation by small RNAs, we identified six ambient temperature-responsive miRNAs (miR156, miR163, miR169, miR172, miR398 and miR399) in Arabidopsis via miRNA microarray and northern hybridization analyses. We also determined the expression profile of 120 unique miRNA loci in response to ambient temperature changes by miRNA northern hybridization analysis. The expression of the ambient temperature-responsive miRNAs and their target genes was largely anticorrelated at two different temperatures (16 and 23oC). Interestingly, a lesion in short vegetative phase (SVP), a key regulator within the thermosensory pathway, caused alteration in the expression of miR172 and a subset of its target genes, providing a link between a thermosensory pathway gene and miR172. The miR172-overexpressing plants showed a temperatureindependent early flowering phenotype, suggesting that modulation of miR172 expression leads to temperature insensitivity. Taken together, our results suggest a genetic framework for flowering-time regulation by ambient temperature-responsive miRNAs under non-stress temperature conditions.
AB - Flowering is the primary trait affected by ambient temperature changes. Plant microRNAs (miRNAs) are small non-coding RNAs playing an important regulatory role in plant development. In this study, to elucidate the mechanism of flowering-time regulation by small RNAs, we identified six ambient temperature-responsive miRNAs (miR156, miR163, miR169, miR172, miR398 and miR399) in Arabidopsis via miRNA microarray and northern hybridization analyses. We also determined the expression profile of 120 unique miRNA loci in response to ambient temperature changes by miRNA northern hybridization analysis. The expression of the ambient temperature-responsive miRNAs and their target genes was largely anticorrelated at two different temperatures (16 and 23oC). Interestingly, a lesion in short vegetative phase (SVP), a key regulator within the thermosensory pathway, caused alteration in the expression of miR172 and a subset of its target genes, providing a link between a thermosensory pathway gene and miR172. The miR172-overexpressing plants showed a temperatureindependent early flowering phenotype, suggesting that modulation of miR172 expression leads to temperature insensitivity. Taken together, our results suggest a genetic framework for flowering-time regulation by ambient temperature-responsive miRNAs under non-stress temperature conditions.
UR - http://www.scopus.com/inward/record.url?scp=77953245655&partnerID=8YFLogxK
U2 - 10.1093/nar/gkp1240
DO - 10.1093/nar/gkp1240
M3 - Article
C2 - 20110261
AN - SCOPUS:77953245655
SN - 0305-1048
VL - 38
SP - 3081
EP - 3093
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - 9
M1 - gkp1240
ER -