Genetic variation of hepatitis B surface antigen coding region among infants with chronic hepatitis B virus infection

Omana V. Nainan; Marina L. Khristova; Kwansoo Byun; Guoliang Xia; Patricia E. Taylor; Cladd E. Stevens; Harold S. Margolis

doi:10.1002/jmv.10206

Genetic variation of hepatitis B surface antigen coding region among infants with chronic hepatitis B virus infection

Omana V. Nainan, Marina L. Khristova, Kwansoo Byun, Guoliang Xia, Patricia E. Taylor, Cladd E. Stevens, Harold S. Margolis

Research output: Contribution to journal › Article › peer-review

82 Citations (Scopus)

Abstract

Variants in the amino acid composition of the primary antibody-binding site of hepatitis B surface antigen (HBsAg) have been identified in a number of populations with chronic hepatitis B virus (HBV) infection. Direct sequencing of amplified or cloned PCR products, solid phase detection of sequence-specific PCR products (SP-PCR), and limiting dilution cloning PCR (LDC-PCR) were compared to determine their sensitivity in detecting differing concentrations of HBsAg variants. LDC-PCR had the greatest sensitivity and could detect HBsAg variants at a concentration of 0.1% of the total viral population. HBsAg variants were detected in 51% of infants with chronic HBV infection acquired after postexposure prophylaxis, and more than half of the variants were detected only by the most sensitive methods.

Original language	English
Pages (from-to)	319-327
Number of pages	9
Journal	Journal of Medical Virology
Volume	68
Issue number	3
DOIs	https://doi.org/10.1002/jmv.10206
Publication status	Published - 2002 Nov 1
Externally published	Yes

Keywords

"a" determinant mutants
Detection of HBsAg variants
HBsAg variants
Hepatitis B vaccine
Postexposure prophylaxis

ASJC Scopus subject areas

Virology
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/jmv.10206

Cite this

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title = "Genetic variation of hepatitis B surface antigen coding region among infants with chronic hepatitis B virus infection",

abstract = "Variants in the amino acid composition of the primary antibody-binding site of hepatitis B surface antigen (HBsAg) have been identified in a number of populations with chronic hepatitis B virus (HBV) infection. Direct sequencing of amplified or cloned PCR products, solid phase detection of sequence-specific PCR products (SP-PCR), and limiting dilution cloning PCR (LDC-PCR) were compared to determine their sensitivity in detecting differing concentrations of HBsAg variants. LDC-PCR had the greatest sensitivity and could detect HBsAg variants at a concentration of 0.1% of the total viral population. HBsAg variants were detected in 51% of infants with chronic HBV infection acquired after postexposure prophylaxis, and more than half of the variants were detected only by the most sensitive methods.",

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author = "Nainan, {Omana V.} and Khristova, {Marina L.} and Kwansoo Byun and Guoliang Xia and Taylor, {Patricia E.} and Stevens, {Cladd E.} and Margolis, {Harold S.}",

year = "2002",

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T1 - Genetic variation of hepatitis B surface antigen coding region among infants with chronic hepatitis B virus infection

AU - Nainan, Omana V.

AU - Khristova, Marina L.

AU - Byun, Kwansoo

AU - Xia, Guoliang

AU - Taylor, Patricia E.

AU - Stevens, Cladd E.

AU - Margolis, Harold S.

PY - 2002/11/1

Y1 - 2002/11/1

N2 - Variants in the amino acid composition of the primary antibody-binding site of hepatitis B surface antigen (HBsAg) have been identified in a number of populations with chronic hepatitis B virus (HBV) infection. Direct sequencing of amplified or cloned PCR products, solid phase detection of sequence-specific PCR products (SP-PCR), and limiting dilution cloning PCR (LDC-PCR) were compared to determine their sensitivity in detecting differing concentrations of HBsAg variants. LDC-PCR had the greatest sensitivity and could detect HBsAg variants at a concentration of 0.1% of the total viral population. HBsAg variants were detected in 51% of infants with chronic HBV infection acquired after postexposure prophylaxis, and more than half of the variants were detected only by the most sensitive methods.

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KW - "a" determinant mutants

KW - Detection of HBsAg variants

KW - HBsAg variants

KW - Hepatitis B vaccine

KW - Postexposure prophylaxis

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Genetic variation of hepatitis B surface antigen coding region among infants with chronic hepatitis B virus infection

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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