Genetically modified Bifidobacterium displaying Salmonella-antigen protects mice from lethal challenge of Salmonella Typhimurium in a murine typhoid fever model

Sakura Yamamoto, Jun Wada, Takane Katayama, Takumi Jikimoto, Masakuni Nakamura, Shohiro Kinoshita, Kyung Mi Lee, Masato Kawabata, Toshiro Shirakawa

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)

Abstract

We developed a novel vaccine platform utilizing Bifidobacterium as an antigen delivery vehicle for mucosal immunization. Genetically modified Bifidobacterium longum displaying Salmonella-flagellin on the cell surface was constructed for the oral typhoid vaccine. The efficiency of this vaccine was evaluated in a murine model of typhoid fever. We then orally administered 2.5×107 CFU of the recombinant Bifidobacterium longum (vaccine) or parental Bifidobacterium longum, or PBS to BALB/C mice every other day for 2 weeks. After the administration, a total of 42 mice (14 mice in each group) were challenged with Salmonella Typhimurium (1.0×107 CFU/mouse). While 12 mice in the PBS group, and 9 in the parental Bifidobacterium longum group died (median survival: 14 and 25 days), only two in the vaccine group died. These data support that our genetically modified Bifidobacterium antigen delivery system offers a promising vaccine platform for inducing efficient mucosal immunity.

Original languageEnglish
Pages (from-to)6684-6691
Number of pages8
JournalVaccine
Volume28
Issue number41
DOIs
Publication statusPublished - 2010 Sept

Bibliographical note

Funding Information:
This study was supported in part by a grant-in-aid for Scientific Research (No. 20591201 ) from Japan Society for the Promotion of Science (JSPS) ; a grant from Hyogo Science and Technology Association ; and a grant-in-aid through the Program of Founding Research Centers for Emerging and Reemerging Infectious Diseases , the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan. K-M Lee is supported by a grant from KICOS ( K20704000007-09A0500-00710 ).

Keywords

  • Bifidobacterium
  • Mucosal vaccine
  • Salmonella

ASJC Scopus subject areas

  • Molecular Medicine
  • General Immunology and Microbiology
  • General Veterinary
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Fingerprint

Dive into the research topics of 'Genetically modified Bifidobacterium displaying Salmonella-antigen protects mice from lethal challenge of Salmonella Typhimurium in a murine typhoid fever model'. Together they form a unique fingerprint.

Cite this