Head and neck cancer (HNC)–derived cell lines represent fundamental models for studying the biological mechanisms underlying cancer development and precision therapies. However, mining the genomic information of HNC cells from available databases requires knowledge on bioinformatics and computational skill sets. Here, we developed a user-friendly web resource for exploring, visualizing, and analyzing genomics information of commonly used HNC cell lines. We populated the current version of GENIPAC with 44 HNC cell lines from 3 studies: ORL Series, OPC-22, and H Series. Specifically, the mRNA expressions for all the 3 studies were derived with RNA-seq. The copy number alterations analysis of ORL Series was performed on the Genome Wide Human Cytoscan HD array, while copy number alterations for OPC-22 were derived from whole exome sequencing. Mutations from ORL Series and H Series were derived from RNA-seq information, while OPC-22 was based on whole exome sequencing. All genomic information was preprocessed with customized scripts and underwent data validation and correction through data set validator tools provided by cBioPortal. The clinical and genomic information of 44 HNC cell lines are easily assessable in GENIPAC. The functional utility of GENIPAC was demonstrated with some of the genomic alterations that are commonly reported in HNC, such as TP53, EGFR, CCND1, and PIK3CA. We showed that these genomic alterations as reported in The Cancer Genome Atlas database were recapitulated in the HNC cell lines in GENIPAC. Importantly, genomic alterations within pathways could be simultaneously visualized. We developed GENIPAC to create access to genomic information on HNC cell lines. This cancer omics initiative will help the research community to accelerate better understanding of HNC and the development of new precision therapeutic options for HNC treatment. GENIPAC is freely available at http://genipac.cancerresearch.my/.
Bibliographical noteFunding Information:
We thank Dr. Daniel Martin for providing data and for technical assistance. We also gratefully acknowledge the Data Intensive Computing Centre of the University of Malaya for providing infrastructure for hosting GENIPAC. B.K.B. Lee gratefully acknowledges PhD funding from the Ong Hin Tiang and Ong Sek Pek Foundation. This study was funded by a High Impact Research Grant from the University of Malaya (UM.C/625/1/HIR/MOHE/ DENT-03) and other sponsors of Cancer Research Malaysia (a nonprofit research organization). We are committed to an understanding of cancer prevention, diagnosis, and treatment through a fundamental research program. The authors declare no potential conflicts of interest with respect to the authorship and/or publication of this article.
© 2018, International & American Associations for Dental Research 2018.
- cancer biology
- gene expression
- oral carcinogenesis
ASJC Scopus subject areas
- General Dentistry