Genome editing methods in animal models

Hyunji Lee; Da Eun Yoon; Kyoungmi Kim

doi:10.1080/19768354.2020.1726462

Genome editing methods in animal models

Hyunji Lee, Da Eun Yoon, Kyoungmi Kim

Research output: Contribution to journal › Review article › peer-review

40 Citations (Scopus)

Abstract

Genetically engineered animal models that reproduce human diseases are very important for the pathological study of various conditions. The development of the clustered regularly interspaced short palindromic repeats (CRISPR) system has enabled a faster and cheaper production of animal models compared with traditional gene-targeting methods using embryonic stem cells. Genome editing tools based on the CRISPR-Cas9 system are a breakthrough technology that allows the precise introduction of mutations at the target DNA sequences. In particular, this accelerated the creation of animal models, and greatly contributed to the research that utilized them. In this review, we introduce various strategies based on the CRISPR-Cas9 system for building animal models of human diseases and describe various in vivo delivery methods of CRISPR-Cas9 that are applied to disease models for therapeutic purposes. In addition, we summarize the currently available animal models of human diseases that were generated using the CRISPR-Cas9 system and discuss future directions.

Original language	English
Pages (from-to)	8-16
Number of pages	9
Journal	Animal Cells and Systems
Volume	24
Issue number	1
DOIs	https://doi.org/10.1080/19768354.2020.1726462
Publication status	Published - 2020 Jan 2

Bibliographical note

Funding Information:
This study was supported by Chung Yang, Cha Young Sun & Jang Hi Joo Memorial fund and Korea university grant (K1923811). And this study was supported by the Bio & Medical Technology Development Program of the National Research Foundation (NRF) of Korea (NRF-2019R1A2C2087198, NRF- 2014M3A9D5A01075128, NRF- 2018M3A9H3021707, and NRF- 2019M3A9H1103792). We thank Chae Eun Yoon for helpful and clear figure drawing on this paper.

Publisher Copyright:
© 2020, © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

Keywords

CRISPR-Cas9 system
In vivo delivery
animal model
genome editing

ASJC Scopus subject areas

Animal Science and Zoology
General Biochemistry,Genetics and Molecular Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1080/19768354.2020.1726462

Cite this

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abstract = "Genetically engineered animal models that reproduce human diseases are very important for the pathological study of various conditions. The development of the clustered regularly interspaced short palindromic repeats (CRISPR) system has enabled a faster and cheaper production of animal models compared with traditional gene-targeting methods using embryonic stem cells. Genome editing tools based on the CRISPR-Cas9 system are a breakthrough technology that allows the precise introduction of mutations at the target DNA sequences. In particular, this accelerated the creation of animal models, and greatly contributed to the research that utilized them. In this review, we introduce various strategies based on the CRISPR-Cas9 system for building animal models of human diseases and describe various in vivo delivery methods of CRISPR-Cas9 that are applied to disease models for therapeutic purposes. In addition, we summarize the currently available animal models of human diseases that were generated using the CRISPR-Cas9 system and discuss future directions.",

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Genome editing methods in animal models

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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