Genome-wide molecular phylogenetic analyses and mating experiments which reveal the evolutionary history and an intermediate stage of speciation of a giant water bug

Tomoya Suzuki, Koki Yano, Shin ya Ohba, Keisuke Kawano, Kazuki Sekiné, Yeon Jae Bae, Koji Tojo

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5 Citations (Scopus)

Abstract

The intermediate stages of speciation are important for understanding the processes involved in the creation of biodiversity, and also comprise a number of interesting phenomena. However, difficulties are associated with dividing clear speciation stages because speciation is a continuous process. Therefore, the elucidation of speciation is an interesting and important task in evolutionary biology. We herein present an example of a species in an intermediate stage of speciation using the giant water bug Appasus japonicus (Heteroptera, Belostomatidae) that was investigated using mating experiments and phylogenetic analyses of the mtDNA COI (658 bp) and 16S rRNA (435 bp) regions, and nDNA SSR (13 loci) and its genome-wide SNPs (11,241 SNPs). The results of our phylogenetic analyses based on their mtDNA data set and the genome-wide SNPs data set strongly supported the paraphyly of the Japanese populations. Therefore, it is suggested that their ancestral lineage which being distributed in the Japanese Archipelago subsequently migrated to the Eurasian Continent (i.e., back-dispersal occurred). Furthermore, the results of the mating experiments suggested that among A. japonicus, even between closely related lineages, premating reproductive isolation has been established by the differentiation of copulatory organ morphologies. In contrast, premating reproductive isolation is not established in the absence of the differentiation of copulatory organ morphologies, even if genetic differentiation is prominent. These results suggested that their phylogenetic distance does not predict premating reproductive isolation. Furthermore, in the present study, we present a clear example of premating reproductive isolation driving speciation between closely related lineages.

Original languageEnglish
Pages (from-to)5179-5195
Number of pages17
JournalMolecular Ecology
Volume30
Issue number20
DOIs
Publication statusPublished - 2021 Oct

Bibliographical note

Funding Information:
We thank Professor Emeritus K. Suzuki of Shinshu University for arranging the study environment. We also express our thanks to Dr M. Takenaka (University of Tsukuba), Mr M. Shimono (Iwakuni City) and Ms H. Yamazaki (Shinshu University) for their cooperation with the field research and collection of specimens, Mr K. Yoneta, Mr T. Sato and Mr M. Murakami for their cooperation with the molecular experiments, and Mr K. Mikami, Mr O. Ohnishi, Mr T. Sekiya, Mr T. Ohtani, and Mr M. Tamura of Shinshu University for their cooperation with keeping the materials used for mating experiments. We are sincerely thankful to Dr M. D. Scherz (University of Potsdam) for his many valuable comments on this study. This study was supported by JSPS KAKENHI Grant Numbers JP20687005, JP23657046, JP16K14807 (K.T.), 285211031 (K.T.), and JP26891010, JP19K16209 (T.S.), the River Fund of The River Foundation, Japan (27-1215-013; K.T.), and the Sasakawa Scientific Research Grant (26-529; T.S.).

Funding Information:
We thank Professor Emeritus K. Suzuki of Shinshu University for arranging the study environment. We also express our thanks to Dr M. Takenaka (University of Tsukuba), Mr M. Shimono (Iwakuni City) and Ms H. Yamazaki (Shinshu University) for their cooperation with the field research and collection of specimens, Mr K. Yoneta, Mr T. Sato and Mr M. Murakami for their cooperation with the molecular experiments, and Mr K. Mikami, Mr O. Ohnishi, Mr T. Sekiya, Mr T. Ohtani, and Mr M. Tamura of Shinshu University for their cooperation with keeping the materials used for mating experiments. We are sincerely thankful to Dr M. D. Scherz (University of Potsdam) for his many valuable comments on this study. This study was supported by JSPS KAKENHI Grant Numbers JP20687005, JP23657046, JP16K14807 (K.T.), 285211031 (K.T.), and JP26891010, JP19K16209 (T.S.), the River Fund of The River Foundation, Japan (27‐1215‐013; K.T.), and the Sasakawa Scientific Research Grant (26‐529; T.S.).

Publisher Copyright:
© 2021 John Wiley & Sons Ltd

Keywords

  • Belostomatidae
  • biogeography
  • reproductive isolation
  • reproductive organ
  • speciation

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics

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