Genomic sequence analysis of virulent Cronobacter sakazakii bacteriophage ES2

Young Duck Lee; Jong Hyun Park; Hyo Ihl Chang

doi:10.1007/s00705-011-1096-7

Genomic sequence analysis of virulent Cronobacter sakazakii bacteriophage ES2

Young Duck Lee, Jong Hyun Park, Hyo Ihl Chang

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Virulent Cronobacter sakazakii bacteriophage ES2 was isolated from swine fecal samples, and the genome sequence by was determined GS-Flx. Bacteriophage ES2 had a double-stranded DNA genome with a length of 22,162 bp and a G+C content of 50. 08%. The morphological characteristics under a transmission electron microscope indicated that bacteriophage ES2 belongs to the family Myoviridae. The structural proteins, including the phage coat protein, were separated by SDS-PAGE and identified by Q-TOF. Bioinformatics analysis of the bacteriophage genome revealed 30 putative open reading frames (ORFs). The predicted protein products of the ORFs were determined and described. To our knowledge, the genome of the newly isolated bacteriophage ES2 was not significantly similar to that of any previously reported bacteriophages of members of the family Enterobacteriaceae.

Original language	English
Pages (from-to)	2105-2108
Number of pages	4
Journal	Archives of virology
Volume	156
Issue number	11
DOIs	https://doi.org/10.1007/s00705-011-1096-7
Publication status	Published - 2011 Nov

Bibliographical note

Funding Information:
This research was supported financially by Korea Institute of Planning and Evolution for Biotechnology of Food, Agriculture, Forestry and Fisheries funded by Korean Government (108147-02-1-SB010).

ASJC Scopus subject areas

Virology

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10.1007/s00705-011-1096-7

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title = "Genomic sequence analysis of virulent Cronobacter sakazakii bacteriophage ES2",

abstract = "Virulent Cronobacter sakazakii bacteriophage ES2 was isolated from swine fecal samples, and the genome sequence by was determined GS-Flx. Bacteriophage ES2 had a double-stranded DNA genome with a length of 22,162 bp and a G+C content of 50. 08%. The morphological characteristics under a transmission electron microscope indicated that bacteriophage ES2 belongs to the family Myoviridae. The structural proteins, including the phage coat protein, were separated by SDS-PAGE and identified by Q-TOF. Bioinformatics analysis of the bacteriophage genome revealed 30 putative open reading frames (ORFs). The predicted protein products of the ORFs were determined and described. To our knowledge, the genome of the newly isolated bacteriophage ES2 was not significantly similar to that of any previously reported bacteriophages of members of the family Enterobacteriaceae.",

author = "Lee, {Young Duck} and Park, {Jong Hyun} and Chang, {Hyo Ihl}",

note = "Funding Information: This research was supported financially by Korea Institute of Planning and Evolution for Biotechnology of Food, Agriculture, Forestry and Fisheries funded by Korean Government (108147-02-1-SB010).",

year = "2011",

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doi = "10.1007/s00705-011-1096-7",

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AU - Lee, Young Duck

AU - Park, Jong Hyun

AU - Chang, Hyo Ihl

N1 - Funding Information: This research was supported financially by Korea Institute of Planning and Evolution for Biotechnology of Food, Agriculture, Forestry and Fisheries funded by Korean Government (108147-02-1-SB010).

PY - 2011/11

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N2 - Virulent Cronobacter sakazakii bacteriophage ES2 was isolated from swine fecal samples, and the genome sequence by was determined GS-Flx. Bacteriophage ES2 had a double-stranded DNA genome with a length of 22,162 bp and a G+C content of 50. 08%. The morphological characteristics under a transmission electron microscope indicated that bacteriophage ES2 belongs to the family Myoviridae. The structural proteins, including the phage coat protein, were separated by SDS-PAGE and identified by Q-TOF. Bioinformatics analysis of the bacteriophage genome revealed 30 putative open reading frames (ORFs). The predicted protein products of the ORFs were determined and described. To our knowledge, the genome of the newly isolated bacteriophage ES2 was not significantly similar to that of any previously reported bacteriophages of members of the family Enterobacteriaceae.

AB - Virulent Cronobacter sakazakii bacteriophage ES2 was isolated from swine fecal samples, and the genome sequence by was determined GS-Flx. Bacteriophage ES2 had a double-stranded DNA genome with a length of 22,162 bp and a G+C content of 50. 08%. The morphological characteristics under a transmission electron microscope indicated that bacteriophage ES2 belongs to the family Myoviridae. The structural proteins, including the phage coat protein, were separated by SDS-PAGE and identified by Q-TOF. Bioinformatics analysis of the bacteriophage genome revealed 30 putative open reading frames (ORFs). The predicted protein products of the ORFs were determined and described. To our knowledge, the genome of the newly isolated bacteriophage ES2 was not significantly similar to that of any previously reported bacteriophages of members of the family Enterobacteriaceae.

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Genomic sequence analysis of virulent Cronobacter sakazakii bacteriophage ES2

Abstract

Bibliographical note

ASJC Scopus subject areas

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