Germinated Brown Rice Extract Inhibits Adipogenesis Through the Down-regulation of Adipogenic Genes in 3T3-L1 Adipocytes

Jin Nyoung Ho; Mi Eun Son; Won Chul Lim; Seung Taik Lim; Hong Yon Cho

doi:10.1007/s11130-013-0366-9

Germinated Brown Rice Extract Inhibits Adipogenesis Through the Down-regulation of Adipogenic Genes in 3T3-L1 Adipocytes

Jin Nyoung Ho, Mi Eun Son, Won Chul Lim, Seung Taik Lim, Hong Yon Cho

Department of Biotechnology

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

The aim of this study was to examine the anti-adipogenic effect of germinated brown rice methanol extract (GBR) in 3T3-L1 adipocytes. The GBR inhibited adipocyte differentiation was measured by Oil Red O staining and glycerol-3-phosphate dehydrogenase (GPDH) activity in a dose-dependent manner without initiating any cytotoxicity. The mRNA levels of adipogenic transcription factors such as CCAAT/enhancer binding protein (C/EBPα), proliferator-activated receptorγ (PPARγ), and sterol regulatory element-binding protein-1c (SREBP-1c), and adipogenic genes, such as fatty acid synthase (FAS), adipocyte fatty acid-binding protein (aP2), and lipoprotein lipase (LPL), were significantly down-regulated by treatment with GBR when compared to that of untreated control cells. Moreover, tumor necrosis factor-α (TNF-α) and interlukin-6 (IL-6) mRNA expressions were attenuated by GBR in mature adipocytes. These data suggest that GBR exhibits an anti-adipogenic effect through the suppression of adipogenesis in 3T3-L1 adipocytes.

Original language	English
Pages (from-to)	274-278
Number of pages	5
Journal	Plant Foods for Human Nutrition
Volume	68
Issue number	3
DOIs	https://doi.org/10.1007/s11130-013-0366-9
Publication status	Published - 2013 Sept

Bibliographical note

Funding Information:
Acknowledgments This work was supported by the Grant of the Korean Research Foundation in the Korean Ministry of Education, Science and Technology (code 2008–0060892).

Keywords

3T3-L1
Adipogenesis
Differentiation
Germinated brown rice

ASJC Scopus subject areas

Food Science
Chemistry (miscellaneous)

Access to Document

10.1007/s11130-013-0366-9

Cite this

@article{833f61d141c144908b3088e2bfedbfea,

title = "Germinated Brown Rice Extract Inhibits Adipogenesis Through the Down-regulation of Adipogenic Genes in 3T3-L1 Adipocytes",

abstract = "The aim of this study was to examine the anti-adipogenic effect of germinated brown rice methanol extract (GBR) in 3T3-L1 adipocytes. The GBR inhibited adipocyte differentiation was measured by Oil Red O staining and glycerol-3-phosphate dehydrogenase (GPDH) activity in a dose-dependent manner without initiating any cytotoxicity. The mRNA levels of adipogenic transcription factors such as CCAAT/enhancer binding protein (C/EBPα), proliferator-activated receptorγ (PPARγ), and sterol regulatory element-binding protein-1c (SREBP-1c), and adipogenic genes, such as fatty acid synthase (FAS), adipocyte fatty acid-binding protein (aP2), and lipoprotein lipase (LPL), were significantly down-regulated by treatment with GBR when compared to that of untreated control cells. Moreover, tumor necrosis factor-α (TNF-α) and interlukin-6 (IL-6) mRNA expressions were attenuated by GBR in mature adipocytes. These data suggest that GBR exhibits an anti-adipogenic effect through the suppression of adipogenesis in 3T3-L1 adipocytes.",

keywords = "3T3-L1, Adipogenesis, Differentiation, Germinated brown rice",

author = "Ho, {Jin Nyoung} and Son, {Mi Eun} and Lim, {Won Chul} and Lim, {Seung Taik} and Cho, {Hong Yon}",

note = "Funding Information: Acknowledgments This work was supported by the Grant of the Korean Research Foundation in the Korean Ministry of Education, Science and Technology (code 2008–0060892).",

year = "2013",

month = sep,

doi = "10.1007/s11130-013-0366-9",

language = "English",

volume = "68",

pages = "274--278",

journal = "Plant Foods for Human Nutrition",

issn = "0921-9668",

publisher = "Springer Netherlands",

number = "3",

}

TY - JOUR

T1 - Germinated Brown Rice Extract Inhibits Adipogenesis Through the Down-regulation of Adipogenic Genes in 3T3-L1 Adipocytes

AU - Ho, Jin Nyoung

AU - Son, Mi Eun

AU - Lim, Won Chul

AU - Lim, Seung Taik

AU - Cho, Hong Yon

N1 - Funding Information: Acknowledgments This work was supported by the Grant of the Korean Research Foundation in the Korean Ministry of Education, Science and Technology (code 2008–0060892).

PY - 2013/9

Y1 - 2013/9

N2 - The aim of this study was to examine the anti-adipogenic effect of germinated brown rice methanol extract (GBR) in 3T3-L1 adipocytes. The GBR inhibited adipocyte differentiation was measured by Oil Red O staining and glycerol-3-phosphate dehydrogenase (GPDH) activity in a dose-dependent manner without initiating any cytotoxicity. The mRNA levels of adipogenic transcription factors such as CCAAT/enhancer binding protein (C/EBPα), proliferator-activated receptorγ (PPARγ), and sterol regulatory element-binding protein-1c (SREBP-1c), and adipogenic genes, such as fatty acid synthase (FAS), adipocyte fatty acid-binding protein (aP2), and lipoprotein lipase (LPL), were significantly down-regulated by treatment with GBR when compared to that of untreated control cells. Moreover, tumor necrosis factor-α (TNF-α) and interlukin-6 (IL-6) mRNA expressions were attenuated by GBR in mature adipocytes. These data suggest that GBR exhibits an anti-adipogenic effect through the suppression of adipogenesis in 3T3-L1 adipocytes.

AB - The aim of this study was to examine the anti-adipogenic effect of germinated brown rice methanol extract (GBR) in 3T3-L1 adipocytes. The GBR inhibited adipocyte differentiation was measured by Oil Red O staining and glycerol-3-phosphate dehydrogenase (GPDH) activity in a dose-dependent manner without initiating any cytotoxicity. The mRNA levels of adipogenic transcription factors such as CCAAT/enhancer binding protein (C/EBPα), proliferator-activated receptorγ (PPARγ), and sterol regulatory element-binding protein-1c (SREBP-1c), and adipogenic genes, such as fatty acid synthase (FAS), adipocyte fatty acid-binding protein (aP2), and lipoprotein lipase (LPL), were significantly down-regulated by treatment with GBR when compared to that of untreated control cells. Moreover, tumor necrosis factor-α (TNF-α) and interlukin-6 (IL-6) mRNA expressions were attenuated by GBR in mature adipocytes. These data suggest that GBR exhibits an anti-adipogenic effect through the suppression of adipogenesis in 3T3-L1 adipocytes.

KW - 3T3-L1

KW - Adipogenesis

KW - Differentiation

KW - Germinated brown rice

UR - http://www.scopus.com/inward/record.url?scp=84883261486&partnerID=8YFLogxK

U2 - 10.1007/s11130-013-0366-9

DO - 10.1007/s11130-013-0366-9

M3 - Article

C2 - 23907589

AN - SCOPUS:84883261486

SN - 0921-9668

VL - 68

SP - 274

EP - 278

JO - Plant Foods for Human Nutrition

JF - Plant Foods for Human Nutrition

IS - 3

ER -

Germinated Brown Rice Extract Inhibits Adipogenesis Through the Down-regulation of Adipogenic Genes in 3T3-L1 Adipocytes

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this