To address its value as a screening tool in the development of antiviral drugs, a recombinant influenza virus expressing green fluorescent protein (rPR8-GFP virus) was investigated in vitro and in vivo. The inhibition of viral growth by a neuraminidase inhibitor in the cells or lower respiratory tracts of mice could be visualized by the level of fluorescence. In addition, the rPR8-GFP virus exhibited high pathogenicity in mice. Taken together, these results suggest that the rPR8-GFP virus can be a useful tool for the rapid identification of antiviral drugs active against influenza viruses.
Bibliographical noteFunding Information:
This study was supported by a grant from the Korea Healthcare Technology R&D Project of the Ministry of Health & Welfare‚ Republic of Korea (Grant No. A103001). We would like to thank Dr. Adolfo Garcia-Sastre for providing us with the rPR8-GFP virus and Sulhwa Jung for technical assistance with the animal experiments. We would also like to thank the Korea Basic Science Institute, Chuncheon Center, for providing us with support with the IVIS-200 series imaging system.
- antiviral drug
- green fluorescent protein
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology