Glucose/Xylose Co-Fermenting Saccharomyces cerevisiae Increases the Production of Acetyl-CoA Derived n-Butanol From Lignocellulosic Biomass

Yeon Jung Lee, Phuong Hoang Nguyen Tran, Ja Kyong Ko, Gyeongtaek Gong, Youngsoon Um, Sung Ok Han, Sun Mi Lee

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Efficient xylose catabolism in engineered Saccharomyces cerevisiae enables more economical lignocellulosic biorefinery with improved production yields per unit of biomass. Yet, the product profile of glucose/xylose co-fermenting S. cerevisiae is mainly limited to bioethanol and a few other chemicals. Here, we introduced an n-butanol-biosynthesis pathway into a glucose/xylose co-fermenting S. cerevisiae strain (XUSEA) to evaluate its potential on the production of acetyl-CoA derived products. Higher n-butanol production of glucose/xylose co-fermenting strain was explained by the transcriptomic landscape, which revealed strongly increased acetyl-CoA and NADPH pools when compared to a glucose fermenting wild-type strain. The acetate supplementation expected to support acetyl-CoA pool further increased n-butanol production, which was also validated during the fermentation of lignocellulosic hydrolysates containing acetate. Our findings imply the feasibility of lignocellulosic biorefinery for producing fuels and chemicals derived from a key intermediate of acetyl-CoA through glucose/xylose co-fermentation.

Original languageEnglish
Article number826787
JournalFrontiers in Bioengineering and Biotechnology
Publication statusPublished - 2022 Feb 16

Bibliographical note

Funding Information:
This research was supported by the Korea Institute of Science and Technology (KIST) Institutional Program (grant number. 2E31853) and the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (Information and Communication Technology) (grant number. NRF-2020M1A2A2080847).

Publisher Copyright:
Copyright © 2022 Lee, Hoang Nguyen Tran, Ko, Gong, Um, Han and Lee.


  • Saccharomyces cerevisiae
  • acetate
  • acetyl-CoA
  • glucose/xylose co-fermentation
  • lignocellulosic biomass
  • n-butanol

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Histology
  • Biomedical Engineering


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