Gold nanostar based biosensor detects epigenetic alterations on promoter of real cells

Anh H. Nguyen; Xingyi Ma; Sang Jun Sim

doi:10.1016/j.bios.2014.12.015

Gold nanostar based biosensor detects epigenetic alterations on promoter of real cells

Anh H. Nguyen, Xingyi Ma, Sang Jun Sim

Department of Chemical and Biological Engineering

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

Epigenetic changes, particularly in cancer suppressor genes, are novel biomarkers for cancer diagnostics and therapeutics. However, epigenetic studies should not only provide an estimation of the amount of 5-methylcytosine, but also examine the presence of epigenetic proteins to reveal the complete epigenetic alterations for downstream molecular process. The challenge of natural epigenetics is to unveil key factors of epigenetics in one assay, containing low concentrations. This would be valuable for the monitoring of early-stage cancer. On the basic of the nanoplasmonic biosensor, here we report a sensitive sensor to study epigenetics of DNA promoter. The results show detection limit for dual epigenetic biomarkers methyl-CpG group and methyl-CpG binding domain protein 2 (MBD2) are one 5-methylcytosine molecule and 125. fM MBD2. Moreover, DNA structure bending, steric competition under interaction of epigenetic proteins and transcription factors; and epigenetics-mediated suppression of transcription are observed during epigenetic alterations. This study provides a platform for full story of epigenetics, as compared with that of methylcytosine-based techniques only.

Original language	English
Pages (from-to)	497-503
Number of pages	7
Journal	Biosensors and Bioelectronics
Volume	66
DOIs	https://doi.org/10.1016/j.bios.2014.12.015
Publication status	Published - 2015 Apr 5

Keywords

DNA methylation
Epigenetics
Gold nanostars
Peptide nucleic acid
Plasmon coupling
Plasmonic biosensors

ASJC Scopus subject areas

Biotechnology
Biophysics
Biomedical Engineering
Electrochemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.bios.2014.12.015

Cite this

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title = "Gold nanostar based biosensor detects epigenetic alterations on promoter of real cells",

abstract = "Epigenetic changes, particularly in cancer suppressor genes, are novel biomarkers for cancer diagnostics and therapeutics. However, epigenetic studies should not only provide an estimation of the amount of 5-methylcytosine, but also examine the presence of epigenetic proteins to reveal the complete epigenetic alterations for downstream molecular process. The challenge of natural epigenetics is to unveil key factors of epigenetics in one assay, containing low concentrations. This would be valuable for the monitoring of early-stage cancer. On the basic of the nanoplasmonic biosensor, here we report a sensitive sensor to study epigenetics of DNA promoter. The results show detection limit for dual epigenetic biomarkers methyl-CpG group and methyl-CpG binding domain protein 2 (MBD2) are one 5-methylcytosine molecule and 125. fM MBD2. Moreover, DNA structure bending, steric competition under interaction of epigenetic proteins and transcription factors; and epigenetics-mediated suppression of transcription are observed during epigenetic alterations. This study provides a platform for full story of epigenetics, as compared with that of methylcytosine-based techniques only.",

keywords = "DNA methylation, Epigenetics, Gold nanostars, Peptide nucleic acid, Plasmon coupling, Plasmonic biosensors",

author = "Nguyen, {Anh H.} and Xingyi Ma and Sim, {Sang Jun}",

note = "Funding Information: This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government ( Ministry of Science, ICT & Future Planning ) (Grant no. NRF-2013R1A2A1A01015 644/2010-0027955 ), and the Korea CCS R&D Center grant funded by the Korea government (Ministry of Science, ICT & Future Planning) (Grant no. 2014M1A8A1049278 ) of the Republic of Korea. Publisher Copyright: {\textcopyright} 2014 Elsevier B.V.",

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doi = "10.1016/j.bios.2014.12.015",

language = "English",

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journal = "Biosensors and Bioelectronics",

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T1 - Gold nanostar based biosensor detects epigenetic alterations on promoter of real cells

AU - Nguyen, Anh H.

AU - Ma, Xingyi

AU - Sim, Sang Jun

N1 - Funding Information: This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government ( Ministry of Science, ICT & Future Planning ) (Grant no. NRF-2013R1A2A1A01015 644/2010-0027955 ), and the Korea CCS R&D Center grant funded by the Korea government (Ministry of Science, ICT & Future Planning) (Grant no. 2014M1A8A1049278 ) of the Republic of Korea. Publisher Copyright: © 2014 Elsevier B.V.

PY - 2015/4/5

Y1 - 2015/4/5

N2 - Epigenetic changes, particularly in cancer suppressor genes, are novel biomarkers for cancer diagnostics and therapeutics. However, epigenetic studies should not only provide an estimation of the amount of 5-methylcytosine, but also examine the presence of epigenetic proteins to reveal the complete epigenetic alterations for downstream molecular process. The challenge of natural epigenetics is to unveil key factors of epigenetics in one assay, containing low concentrations. This would be valuable for the monitoring of early-stage cancer. On the basic of the nanoplasmonic biosensor, here we report a sensitive sensor to study epigenetics of DNA promoter. The results show detection limit for dual epigenetic biomarkers methyl-CpG group and methyl-CpG binding domain protein 2 (MBD2) are one 5-methylcytosine molecule and 125. fM MBD2. Moreover, DNA structure bending, steric competition under interaction of epigenetic proteins and transcription factors; and epigenetics-mediated suppression of transcription are observed during epigenetic alterations. This study provides a platform for full story of epigenetics, as compared with that of methylcytosine-based techniques only.

AB - Epigenetic changes, particularly in cancer suppressor genes, are novel biomarkers for cancer diagnostics and therapeutics. However, epigenetic studies should not only provide an estimation of the amount of 5-methylcytosine, but also examine the presence of epigenetic proteins to reveal the complete epigenetic alterations for downstream molecular process. The challenge of natural epigenetics is to unveil key factors of epigenetics in one assay, containing low concentrations. This would be valuable for the monitoring of early-stage cancer. On the basic of the nanoplasmonic biosensor, here we report a sensitive sensor to study epigenetics of DNA promoter. The results show detection limit for dual epigenetic biomarkers methyl-CpG group and methyl-CpG binding domain protein 2 (MBD2) are one 5-methylcytosine molecule and 125. fM MBD2. Moreover, DNA structure bending, steric competition under interaction of epigenetic proteins and transcription factors; and epigenetics-mediated suppression of transcription are observed during epigenetic alterations. This study provides a platform for full story of epigenetics, as compared with that of methylcytosine-based techniques only.

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KW - Peptide nucleic acid

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Gold nanostar based biosensor detects epigenetic alterations on promoter of real cells

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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