TY - JOUR
T1 - GPR1 regulates filamentous growth through FLO11 in yeast Saccharomyces cerevisiae
AU - Tamaki, Hisanori
AU - Miwa, Takuya
AU - Shinozaki, Makiko
AU - Saito, Megumi
AU - Yun, Cheol Won
AU - Yamamoto, Kenji
AU - Kumagai, Hidehiko
N1 - Funding Information:
We thank Drs. M. Lorentz, J. Heitman, and J. H. Hegemann for generously providing yeast strains and plasmids. This work was supported by Grants-in-Aid from the Ministry of Education, Science, and Culture of Japan.
PY - 2000/1/7
Y1 - 2000/1/7
N2 - Cell growth and differentiation are regulated by nutrient availability in the yeast Saccharomyces cerevisiae. Under conditions of nitrogen limitation, diploid cells of S. cerevisiae differentiate to a filamentous growth known as a pseudohyphal growth, while haploid cells produce invasive filaments which penetrate the agar in nutrient-rich medium. We have found that GPR1, which encodes a putative G-protein-coupled receptor, is required for both pseudohyphal and invasive growth. Pseudohyphal growth was defective in Δgpr1/Δgpr1 mutant strain and this defect was reversed by addition of cAMP. Also, haploid Δgpr1 mutant strain was defective in invasive growth. Northern blot analysis revealed that the transcriptional level of FLO11, which encodes a recently identified cell surface flocculin required for pseudohyphal growth, was reduced in Δgpr1 mutant strain. These results indicate that GPR1 regulates both pseudohyphal and invasive growth by a cAMP-dependent mechanism. (C) 2000 Academic Press.
AB - Cell growth and differentiation are regulated by nutrient availability in the yeast Saccharomyces cerevisiae. Under conditions of nitrogen limitation, diploid cells of S. cerevisiae differentiate to a filamentous growth known as a pseudohyphal growth, while haploid cells produce invasive filaments which penetrate the agar in nutrient-rich medium. We have found that GPR1, which encodes a putative G-protein-coupled receptor, is required for both pseudohyphal and invasive growth. Pseudohyphal growth was defective in Δgpr1/Δgpr1 mutant strain and this defect was reversed by addition of cAMP. Also, haploid Δgpr1 mutant strain was defective in invasive growth. Northern blot analysis revealed that the transcriptional level of FLO11, which encodes a recently identified cell surface flocculin required for pseudohyphal growth, was reduced in Δgpr1 mutant strain. These results indicate that GPR1 regulates both pseudohyphal and invasive growth by a cAMP-dependent mechanism. (C) 2000 Academic Press.
UR - http://www.scopus.com/inward/record.url?scp=0034614578&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1999.1914
DO - 10.1006/bbrc.1999.1914
M3 - Article
C2 - 10623592
AN - SCOPUS:0034614578
SN - 0006-291X
VL - 267
SP - 164
EP - 168
JO - Biochemical and biophysical research communications
JF - Biochemical and biophysical research communications
IS - 1
ER -