GPR40 Agonism Modulates Inflammatory Reactions in Vascular Endothelial Cells

Joo Won Kim, Eun Roh, Kyung Mook Choi, Hye Jin Yoo, Hwan Jin Hwang, Sei Hyun Baik

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Endothelial dysfunction is strongly linked with inflammatory responses, which can impact cardiovascular disease. Recently, G protein-coupled receptor 40 (GPR40) has been investigated as a modulator of metabolic stress; however, the function of GPR40 in vascular endothelial cells has not been reported. We analyzed whether treatment of GPR40-specific agonists modulated the inflammatory responses in human umbilical vein endothelial cells (HUVECs). Treatment with LY2922470, a GPR40 agonist, significantly reduced lipopolysaccharide (LPS)-mediated nuclear factor-kappa B (NF-κB) phosphorylation and movement into the nucleus from the cytosol. However, treatment with another GPR40 agonist, TAK875, did not inhibit LPS-induced NF-κB activation. LPS treatment induced expression of adhesion molecules vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) and attachment of THP-1 cells to HUVECs, which were all decreased by LY2922470 but not TAK875. Our results showed that ligand-dependent agonism of GPR40 is a promising therapeutic target for overcoming inflammatory reactions in the endothelium.

Original languageEnglish
Pages (from-to)506-511
Number of pages6
JournalDiabetes and Metabolism Journal
Volume46
Issue number3
DOIs
Publication statusPublished - 2022 May

Bibliographical note

Funding Information:
This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF), South Korea, funded by the Ministry of Education (NRF-2020R1I1A1A01072592 and NRF-2021R1A2C2008792).

Publisher Copyright:
Copyright © 2022 Korean Diabetes Association

Keywords

  • Cell adhesion molecules
  • G-protein-coupled 40
  • Human umbilical vein endothelial cells
  • Inflammation; Receptors

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism

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