Gramella salexigens sp. Nov., isolated from seawater

Su Kyoung Shin; Eunji Kim; Hana Yi

doi:10.1099/ijsem.0.002850

Gramella salexigens sp. Nov., isolated from seawater

Su Kyoung Shin, Eunji Kim, Hana Yi

Department of Public Health Sciences

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

A Gram-stain-negative, rod-shaped, aerobic bacterial strain, designated LPB0144^T was isolated from the seawater of South Sea, Korea. The 16S rRNA gene sequence was found to share the highest sequence similarity to Gramella sediminilitoris GHTF-27^T (97.7 %) and the strain branched within the radiation of the genus Gramella in phylogenetic trees. Thus, the taxonomic position of the novel isolate was investigated using a polyphasic approach and complete genome sequencing. Strain LPB0144^T has a circular chromosome of 2.98 Mb with DNA G+C content of 38.2 mol%. The genome includes 2604 protein-coding genes and three copies of rRNA operons. The detected respiratory quinone (MK-6) and the major polar lipid (phosphatidylethanolamine) resemble the chemotaxonomic profile of other Gramella species. The major cellular fatty acid profile (iso-C_{15: 0}, iso-C_{16: 0}, iso-C_{17: 0} 3-OH and anteiso-C_{15: 0}) is also within the range of Gramella, but detailed composition and amounts were found to be different from those of closely related neighbours. Many biochemical and physiological characteristics also distinguished the isolate from other species within the genus Gramella. On the basis of polyphasic taxonomic data obtained here, we propose strain LPB0144^T as a novel Gramella species, for which the name Gramella salexigens sp. nov. is proposed. The type strain is LPB0144^T (=KACC 18894^T=JCM 31560^T).

Original language	English
Article number	002850
Pages (from-to)	2381-2385
Number of pages	5
Journal	International Journal of Systematic and Evolutionary Microbiology
Volume	68
Issue number	7
DOIs	https://doi.org/10.1099/ijsem.0.002850
Publication status	Published - 2018 Jul

Bibliographical note

Funding Information:
This study was supported by the Survey of Korean Indigenous Species Program through the National Institute of Biological Resources (NIBR) funded by the Korean Ministry of Environment, by a National Research Council of Science and Technology (NST) grant from the Korean Government (MSIP; No. CRC-16-01-KRICT) and by a Korea University Grant (No. K1508351).

Keywords

Genome
Gmella salexigens
Gramella
Novel species

ASJC Scopus subject areas

Microbiology
Ecology, Evolution, Behavior and Systematics

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10.1099/ijsem.0.002850

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@article{9e1492ddce064682947af805120910db,

title = "Gramella salexigens sp. Nov., isolated from seawater",

abstract = "A Gram-stain-negative, rod-shaped, aerobic bacterial strain, designated LPB0144T was isolated from the seawater of South Sea, Korea. The 16S rRNA gene sequence was found to share the highest sequence similarity to Gramella sediminilitoris GHTF-27T (97.7 %) and the strain branched within the radiation of the genus Gramella in phylogenetic trees. Thus, the taxonomic position of the novel isolate was investigated using a polyphasic approach and complete genome sequencing. Strain LPB0144T has a circular chromosome of 2.98 Mb with DNA G+C content of 38.2 mol%. The genome includes 2604 protein-coding genes and three copies of rRNA operons. The detected respiratory quinone (MK-6) and the major polar lipid (phosphatidylethanolamine) resemble the chemotaxonomic profile of other Gramella species. The major cellular fatty acid profile (iso-C15: 0, iso-C16: 0, iso-C17: 0 3-OH and anteiso-C15: 0) is also within the range of Gramella, but detailed composition and amounts were found to be different from those of closely related neighbours. Many biochemical and physiological characteristics also distinguished the isolate from other species within the genus Gramella. On the basis of polyphasic taxonomic data obtained here, we propose strain LPB0144T as a novel Gramella species, for which the name Gramella salexigens sp. nov. is proposed. The type strain is LPB0144T (=KACC 18894T=JCM 31560T).",

keywords = "Genome, Gmella salexigens, Gramella, Novel species",

author = "Shin, {Su Kyoung} and Eunji Kim and Hana Yi",

note = "Funding Information: This study was supported by the Survey of Korean Indigenous Species Program through the National Institute of Biological Resources (NIBR) funded by the Korean Ministry of Environment, by a National Research Council of Science and Technology (NST) grant from the Korean Government (MSIP; No. CRC-16-01-KRICT) and by a Korea University Grant (No. K1508351).",

year = "2018",

month = jul,

doi = "10.1099/ijsem.0.002850",

language = "English",

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pages = "2381--2385",

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T1 - Gramella salexigens sp. Nov., isolated from seawater

AU - Shin, Su Kyoung

AU - Kim, Eunji

AU - Yi, Hana

N1 - Funding Information: This study was supported by the Survey of Korean Indigenous Species Program through the National Institute of Biological Resources (NIBR) funded by the Korean Ministry of Environment, by a National Research Council of Science and Technology (NST) grant from the Korean Government (MSIP; No. CRC-16-01-KRICT) and by a Korea University Grant (No. K1508351).

PY - 2018/7

Y1 - 2018/7

N2 - A Gram-stain-negative, rod-shaped, aerobic bacterial strain, designated LPB0144T was isolated from the seawater of South Sea, Korea. The 16S rRNA gene sequence was found to share the highest sequence similarity to Gramella sediminilitoris GHTF-27T (97.7 %) and the strain branched within the radiation of the genus Gramella in phylogenetic trees. Thus, the taxonomic position of the novel isolate was investigated using a polyphasic approach and complete genome sequencing. Strain LPB0144T has a circular chromosome of 2.98 Mb with DNA G+C content of 38.2 mol%. The genome includes 2604 protein-coding genes and three copies of rRNA operons. The detected respiratory quinone (MK-6) and the major polar lipid (phosphatidylethanolamine) resemble the chemotaxonomic profile of other Gramella species. The major cellular fatty acid profile (iso-C15: 0, iso-C16: 0, iso-C17: 0 3-OH and anteiso-C15: 0) is also within the range of Gramella, but detailed composition and amounts were found to be different from those of closely related neighbours. Many biochemical and physiological characteristics also distinguished the isolate from other species within the genus Gramella. On the basis of polyphasic taxonomic data obtained here, we propose strain LPB0144T as a novel Gramella species, for which the name Gramella salexigens sp. nov. is proposed. The type strain is LPB0144T (=KACC 18894T=JCM 31560T).

AB - A Gram-stain-negative, rod-shaped, aerobic bacterial strain, designated LPB0144T was isolated from the seawater of South Sea, Korea. The 16S rRNA gene sequence was found to share the highest sequence similarity to Gramella sediminilitoris GHTF-27T (97.7 %) and the strain branched within the radiation of the genus Gramella in phylogenetic trees. Thus, the taxonomic position of the novel isolate was investigated using a polyphasic approach and complete genome sequencing. Strain LPB0144T has a circular chromosome of 2.98 Mb with DNA G+C content of 38.2 mol%. The genome includes 2604 protein-coding genes and three copies of rRNA operons. The detected respiratory quinone (MK-6) and the major polar lipid (phosphatidylethanolamine) resemble the chemotaxonomic profile of other Gramella species. The major cellular fatty acid profile (iso-C15: 0, iso-C16: 0, iso-C17: 0 3-OH and anteiso-C15: 0) is also within the range of Gramella, but detailed composition and amounts were found to be different from those of closely related neighbours. Many biochemical and physiological characteristics also distinguished the isolate from other species within the genus Gramella. On the basis of polyphasic taxonomic data obtained here, we propose strain LPB0144T as a novel Gramella species, for which the name Gramella salexigens sp. nov. is proposed. The type strain is LPB0144T (=KACC 18894T=JCM 31560T).

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KW - Novel species

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EP - 2385

JO - International Journal of Systematic and Evolutionary Microbiology

JF - International Journal of Systematic and Evolutionary Microbiology

IS - 7

M1 - 002850

ER -

Gramella salexigens sp. Nov., isolated from seawater

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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