Growth factors-loaded stents modified with hyaluronic acid and heparin for induction of rapid and tight re-endothelialization

Dong Hoon Choi; Sung Nam Kang; Seong Min Kim; Samy Gobaa; Bang Ju Park; Ik Hwan Kim; Yoon Ki Joung; Dong Keun Han

doi:10.1016/j.colsurfb.2016.01.028

Growth factors-loaded stents modified with hyaluronic acid and heparin for induction of rapid and tight re-endothelialization

Dong Hoon Choi
, Sung Nam Kang
, Seong Min Kim
, Samy Gobaa
, Bang Ju Park
, Ik Hwan Kim
, Yoon Ki Joung
, Dong Keun Han^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

38 Citations (Scopus)

Abstract

Rapid re-endothelialization of damaged vessel lining efficiently prevents restenosis and thrombosis and restores original vascular functions. In this study, we designed a novel metallic stent with a heparin-modified surface and used different methods, including 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and divinyl sulfone (DVS), to load growth factors. First we loaded heparin into a dopamine-conjugated hyaluronic acid (HA) coating to serve as a growth factor reservoir. In a second step, we took advantage of the heparin-binding domain of vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) to gain advanced re-endothelialization capabilities. We demonstrated that DVS technique offered higher amount of growth factor loading. In vitro assessment also showed better capillary-like structure formation and localized gap junctions when DVS coating was employed. This study suggested that growth factor loaded stent modified by HA and heparin provided the advantage to rapid and tight restoration of endothelium.

Original language	English
Pages (from-to)	602-610
Number of pages	9
Journal	Colloids and Surfaces B: Biointerfaces
Volume	141
DOIs	https://doi.org/10.1016/j.colsurfb.2016.01.028
Publication status	Published - 2016 May 1

Bibliographical note

Funding Information:
This work was supported by Cell Regeneration Program (2013M3A9C6049717) and Pioneer Research Center Program (2014M3C1A3056052) through the National Research Foundation of Korea funded by the Ministry of Science, ICT and Future Planning (MSIP) and Core Material (10048019) funded by Ministry of Trade, Industry and Energy (MOTIE), and KIST Program (2E25260) funded by MSIP, Republic of Korea.

Keywords

Dopamine
Growth factor
Heparin
Hyaluronic acid
Re-endothelialization
Stent

ASJC Scopus subject areas

Biotechnology
Surfaces and Interfaces
Physical and Theoretical Chemistry
Colloid and Surface Chemistry

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10.1016/j.colsurfb.2016.01.028

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title = "Growth factors-loaded stents modified with hyaluronic acid and heparin for induction of rapid and tight re-endothelialization",

abstract = "Rapid re-endothelialization of damaged vessel lining efficiently prevents restenosis and thrombosis and restores original vascular functions. In this study, we designed a novel metallic stent with a heparin-modified surface and used different methods, including 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and divinyl sulfone (DVS), to load growth factors. First we loaded heparin into a dopamine-conjugated hyaluronic acid (HA) coating to serve as a growth factor reservoir. In a second step, we took advantage of the heparin-binding domain of vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) to gain advanced re-endothelialization capabilities. We demonstrated that DVS technique offered higher amount of growth factor loading. In vitro assessment also showed better capillary-like structure formation and localized gap junctions when DVS coating was employed. This study suggested that growth factor loaded stent modified by HA and heparin provided the advantage to rapid and tight restoration of endothelium.",

keywords = "Dopamine, Growth factor, Heparin, Hyaluronic acid, Re-endothelialization, Stent",

author = "Choi, \{Dong Hoon\} and Kang, \{Sung Nam\} and Kim, \{Seong Min\} and Samy Gobaa and Park, \{Bang Ju\} and Kim, \{Ik Hwan\} and Joung, \{Yoon Ki\} and Han, \{Dong Keun\}",

note = "Funding Information: This work was supported by Cell Regeneration Program (2013M3A9C6049717) and Pioneer Research Center Program (2014M3C1A3056052) through the National Research Foundation of Korea funded by the Ministry of Science, ICT and Future Planning (MSIP) and Core Material (10048019) funded by Ministry of Trade, Industry and Energy (MOTIE), and KIST Program (2E25260) funded by MSIP, Republic of Korea.",

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journal = "Colloids and Surfaces B: Biointerfaces",

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AU - Kim, Seong Min

AU - Gobaa, Samy

AU - Park, Bang Ju

AU - Kim, Ik Hwan

AU - Joung, Yoon Ki

AU - Han, Dong Keun

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PY - 2016/5/1

Y1 - 2016/5/1

N2 - Rapid re-endothelialization of damaged vessel lining efficiently prevents restenosis and thrombosis and restores original vascular functions. In this study, we designed a novel metallic stent with a heparin-modified surface and used different methods, including 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and divinyl sulfone (DVS), to load growth factors. First we loaded heparin into a dopamine-conjugated hyaluronic acid (HA) coating to serve as a growth factor reservoir. In a second step, we took advantage of the heparin-binding domain of vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) to gain advanced re-endothelialization capabilities. We demonstrated that DVS technique offered higher amount of growth factor loading. In vitro assessment also showed better capillary-like structure formation and localized gap junctions when DVS coating was employed. This study suggested that growth factor loaded stent modified by HA and heparin provided the advantage to rapid and tight restoration of endothelium.

AB - Rapid re-endothelialization of damaged vessel lining efficiently prevents restenosis and thrombosis and restores original vascular functions. In this study, we designed a novel metallic stent with a heparin-modified surface and used different methods, including 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and divinyl sulfone (DVS), to load growth factors. First we loaded heparin into a dopamine-conjugated hyaluronic acid (HA) coating to serve as a growth factor reservoir. In a second step, we took advantage of the heparin-binding domain of vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) to gain advanced re-endothelialization capabilities. We demonstrated that DVS technique offered higher amount of growth factor loading. In vitro assessment also showed better capillary-like structure formation and localized gap junctions when DVS coating was employed. This study suggested that growth factor loaded stent modified by HA and heparin provided the advantage to rapid and tight restoration of endothelium.

KW - Dopamine

KW - Growth factor

KW - Heparin

KW - Hyaluronic acid

KW - Re-endothelialization

KW - Stent

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Growth factors-loaded stents modified with hyaluronic acid and heparin for induction of rapid and tight re-endothelialization

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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