Abstract
Genome editing has developed rapidly in various research fields for targeted genome modifications in many organisms, including cells, plants, viruses, and animals. The clustered regularly interspaced short palindromic repeats-associated protein 9 system stands as a potent tool in gene editing for generating cells and animal models with high precision. The clinical potential of clustered regularly interspaced short palindromic repeats-associated protein 9 has been extensively reported, with applications in genetic disease correction, inhibition of viral replication, and personalized or targeted therapeutics for various cancers. In this study, we provide a guide on single-guide RNA design, cloning single-guide RNA into plasmid vectors, single-cell isolation via transfection, and identification of knockout clones using next-generation sequencing. In addition, by providing the results of insertion into mammalian cell lines through next-generation sequencing, we offer useful information to those conducting research on human and animal cell lines.
Original language | English |
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Article number | 100087 |
Journal | Molecules and cells |
Volume | 47 |
Issue number | 7 |
DOIs | |
Publication status | Published - 2024 Jul |
Bibliographical note
Publisher Copyright:© 2024 The Authors
Keywords
- Clustered regularly interspaced short palindromic repeats-associated protein 9
- Culture system
- Genome editing
- Single-guide RNA
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology