Guided three-dimensional growth of functional cardiomyocytes on polyethylene glycol nanostructures

Deok Ho Kim, Pilnam Kim, Inseon Song, Jae Min Cha, Sang Ho Lee, Byungkyu Kim, Kahp Y. Suh

Research output: Contribution to journalArticlepeer-review

105 Citations (Scopus)


We introduce well-defined nanopillar arrays of a poly(ethylene glycol) (PEG) hydrogel as a cell culture platform to guide a 3D construct of primary rat cardiomyocytes in vitro for potential tissue engineering applications. Ultraviolet (UV)-assisted capillary lithography was used to fabricate highly uniform ∼150 nm PEG pillars with ∼400 nm height. It was found that cell adhesion was significantly enhanced on PEG nanopillars (132 ± 29 cells/mm 2) compared to that on the bare PEG control (39 ± 17 cells/mm 2) (p < 0.05) but substantially reduced compared to that on the glass control (502 ± 45 cells/mm2) (p < 0.01). Furthermore, in colonizing cardiomyocytes, the nanopillars stimulated self-assembled aggregates among the contacting cells with 3D growth, which is a unique feature for nanopatterned PEG hydrogels as a cell culture substrate. The 3D-grown cardiomyocytes retained their conductive and contractile properties, as evidenced by the observation of beating cardiomyocytes with robust action potential generation.

Original languageEnglish
Pages (from-to)5419-5426
Number of pages8
Issue number12
Publication statusPublished - 2006 Jun 6

ASJC Scopus subject areas

  • General Materials Science
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry


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