TY - JOUR
T1 - Haemocompatibility evaluation of silica nanomaterials using hemorheological measurements
AU - Kim, Jeongho
AU - Heo, Yu Jin
AU - Shin, Sehyun
N1 - Publisher Copyright:
© 2016 - IOS Press and the authors. All rights reserved.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2016/3/18
Y1 - 2016/3/18
N2 - Silica nanomaterials (NMs) are widely used in semiconductor, agriculture, cosmetics, and biomedical applications, in addition to other industries. We investigated the toxic effect of silica NMs on rheological characteristics of human red blood cells (RBCs), including hemolysis, deformability, aggregation, and morphological changes. Red blood cells were exposed to silica nanoparticles (d=~200 nm) or silica nanowires (d=~200 nm, l=1μm or 10μm) at a range of concentrations and incubation times. Rheological characteristics were measured using microfluidic-laser diffractometry and aggregometry. Overall, at a concentration greater than 12.5μg/ml, the hemolytic activity was shown to be in the order of nanoparticles, short nanowires, and long nanowires. Elongation index (EI) values were insignificant in the RBCs exposed to each of the silica NMs at a concentration of 12.5μg/ml. Aggregation index (AI) values decreased in the short silica nanowires at a concentration of 12.5μg/ml compared to other silica NMs. Therefore, the safe concentration of silica NMs for toxicity, in this study, was considered less than 12.5μg/ml. These hemorheological results provided insight into the interaction between RBCs and silica NMs; they will also help assess the risk of NMs' toxicity in the blood.
AB - Silica nanomaterials (NMs) are widely used in semiconductor, agriculture, cosmetics, and biomedical applications, in addition to other industries. We investigated the toxic effect of silica NMs on rheological characteristics of human red blood cells (RBCs), including hemolysis, deformability, aggregation, and morphological changes. Red blood cells were exposed to silica nanoparticles (d=~200 nm) or silica nanowires (d=~200 nm, l=1μm or 10μm) at a range of concentrations and incubation times. Rheological characteristics were measured using microfluidic-laser diffractometry and aggregometry. Overall, at a concentration greater than 12.5μg/ml, the hemolytic activity was shown to be in the order of nanoparticles, short nanowires, and long nanowires. Elongation index (EI) values were insignificant in the RBCs exposed to each of the silica NMs at a concentration of 12.5μg/ml. Aggregation index (AI) values decreased in the short silica nanowires at a concentration of 12.5μg/ml compared to other silica NMs. Therefore, the safe concentration of silica NMs for toxicity, in this study, was considered less than 12.5μg/ml. These hemorheological results provided insight into the interaction between RBCs and silica NMs; they will also help assess the risk of NMs' toxicity in the blood.
KW - Silica
KW - hemorheology
KW - nanomaterials
KW - toxicity
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U2 - 10.3233/CH-151953
DO - 10.3233/CH-151953
M3 - Article
C2 - 26410861
AN - SCOPUS:84961844586
SN - 1386-0291
VL - 62
SP - 99
EP - 107
JO - Clinical Hemorheology and Microcirculation
JF - Clinical Hemorheology and Microcirculation
IS - 2
ER -