Halomonas getboli sp. nov., a halotolerant bacteria isolated from a salt flat

Yeonjae Yoo; Hanbyul Lee; Jong Seong Khim; Xiaoyue Xu; Bogun Kim; In Geol Choi; Jae Jin Kim

doi:10.1099/ijsem.0.005634

Halomonas getboli sp. nov., a halotolerant bacteria isolated from a salt flat

Yeonjae Yoo, Hanbyul Lee, Jong Seong Khim, Xiaoyue Xu, Bogun Kim, In Geol Choi, Jae Jin Kim

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

A novel Gram-stain-negative, rod-shaped, cream-coloured, motile, halotolerant bacterium, designated as YJPS3-2^T, was isolated from saltern sediment of the Yellow sea in Yongyu-do, Republic of Korea. Strain YJPS3-2^T grew at pH 5.0–10.0 (optimum, pH 7.0), 4–40 °C (optimum, 30 °C) and with 1–15% (w/v) NaCl (optimum 3 %). The 16S rRNA gene sequence analysis indicated that strain YJPS3-2^T was closely related to those of Halomonas halophila F5-7^T (98.75 %), Halomonas salina F8-11^T (98.74 %), Halomonas smyrnensis AAD6^T (98.66 %), Halomonas organivorans G-16.1^T (98.34 %), Halomonas kore-ensis SS20^T (97.98 %) and Halomonas beimenensis NTU-107^T (96.93 %). The average nucleotide identity and digital DNA– DNA hybridization values between YJPS3-2^T and related type strains were 86.9–91.6 % and 32.0–44.8 %. Strain YJPS3-2^T was characterized as having Q-9 as the predominant respiratory quinone and the principal fatty acids (>10 %) were C_{16: 0} (31.4 %), C_{19: 0} ω8c cyclo (16.3 %), C_{17: 0} cyclo (11.9 %) and C_{12: 0} 3-OH (10.4 %). The polar lipids consisted of phosphatidylcho-line, diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The DNA G+C content of strain YJPS3-2^T is 68.1mol %. Based on the polyphasic taxonomic evidence presented in this study, YJPS3-2^T should be classified as representing a novel species within the genus Halmonas, for which name Halomonas getboli is proposed, with the type strain YJPS3-2^T (= KCTC 92124^T =KACC 22561^T =JCM 35085^T).

Original language	English
Article number	005634
Journal	International Journal of Systematic and Evolutionary Microbiology
Volume	72
Issue number	12
DOIs	https://doi.org/10.1099/ijsem.0.005634
Publication status	Published - 2022

Bibliographical note

Funding Information:
This research was supported by "Development of living shoreline technology based on blue carbon science toward climate change adaptation" of the Korea Institute of Marine Science & Technology Promotion (KIMST) funded by the Ministry of Oceans and Fisheries (KIMST-20220526)

Publisher Copyright:
© 2022 The Authors.

Keywords

Getbol
Halomonas
halophile
halotolerant bacteria
marine bacteria

ASJC Scopus subject areas

Microbiology
Ecology, Evolution, Behavior and Systematics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1099/ijsem.0.005634

Cite this

@article{3c97a9fcb3a149e48be748becbf9f7c1,

title = "Halomonas getboli sp. nov., a halotolerant bacteria isolated from a salt flat",

abstract = "A novel Gram-stain-negative, rod-shaped, cream-coloured, motile, halotolerant bacterium, designated as YJPS3-2T, was isolated from saltern sediment of the Yellow sea in Yongyu-do, Republic of Korea. Strain YJPS3-2T grew at pH 5.0–10.0 (optimum, pH 7.0), 4–40 °C (optimum, 30 °C) and with 1–15% (w/v) NaCl (optimum 3 %). The 16S rRNA gene sequence analysis indicated that strain YJPS3-2T was closely related to those of Halomonas halophila F5-7T (98.75 %), Halomonas salina F8-11T (98.74 %), Halomonas smyrnensis AAD6T (98.66 %), Halomonas organivorans G-16.1T (98.34 %), Halomonas kore-ensis SS20T (97.98 %) and Halomonas beimenensis NTU-107T (96.93 %). The average nucleotide identity and digital DNA– DNA hybridization values between YJPS3-2T and related type strains were 86.9–91.6 % and 32.0–44.8 %. Strain YJPS3-2T was characterized as having Q-9 as the predominant respiratory quinone and the principal fatty acids (>10 %) were C16: 0 (31.4 %), C19: 0 ω8c cyclo (16.3 %), C17: 0 cyclo (11.9 %) and C12: 0 3-OH (10.4 %). The polar lipids consisted of phosphatidylcho-line, diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The DNA G+C content of strain YJPS3-2T is 68.1mol %. Based on the polyphasic taxonomic evidence presented in this study, YJPS3-2T should be classified as representing a novel species within the genus Halmonas, for which name Halomonas getboli is proposed, with the type strain YJPS3-2T (= KCTC 92124T =KACC 22561T =JCM 35085T).",

keywords = "Getbol, Halomonas, halophile, halotolerant bacteria, marine bacteria",

author = "Yeonjae Yoo and Hanbyul Lee and Khim, {Jong Seong} and Xiaoyue Xu and Bogun Kim and Choi, {In Geol} and Kim, {Jae Jin}",

note = "Funding Information: This research was supported by {"}Development of living shoreline technology based on blue carbon science toward climate change adaptation{"} of the Korea Institute of Marine Science & Technology Promotion (KIMST) funded by the Ministry of Oceans and Fisheries (KIMST-20220526) Publisher Copyright: {\textcopyright} 2022 The Authors.",

year = "2022",

doi = "10.1099/ijsem.0.005634",

language = "English",

volume = "72",

journal = "International Journal of Systematic and Evolutionary Microbiology",

issn = "1466-5026",

publisher = "Microbiology Society",

number = "12",

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TY - JOUR

T1 - Halomonas getboli sp. nov., a halotolerant bacteria isolated from a salt flat

AU - Yoo, Yeonjae

AU - Lee, Hanbyul

AU - Khim, Jong Seong

AU - Xu, Xiaoyue

AU - Kim, Bogun

AU - Choi, In Geol

AU - Kim, Jae Jin

N1 - Funding Information: This research was supported by "Development of living shoreline technology based on blue carbon science toward climate change adaptation" of the Korea Institute of Marine Science & Technology Promotion (KIMST) funded by the Ministry of Oceans and Fisheries (KIMST-20220526) Publisher Copyright: © 2022 The Authors.

PY - 2022

Y1 - 2022

N2 - A novel Gram-stain-negative, rod-shaped, cream-coloured, motile, halotolerant bacterium, designated as YJPS3-2T, was isolated from saltern sediment of the Yellow sea in Yongyu-do, Republic of Korea. Strain YJPS3-2T grew at pH 5.0–10.0 (optimum, pH 7.0), 4–40 °C (optimum, 30 °C) and with 1–15% (w/v) NaCl (optimum 3 %). The 16S rRNA gene sequence analysis indicated that strain YJPS3-2T was closely related to those of Halomonas halophila F5-7T (98.75 %), Halomonas salina F8-11T (98.74 %), Halomonas smyrnensis AAD6T (98.66 %), Halomonas organivorans G-16.1T (98.34 %), Halomonas kore-ensis SS20T (97.98 %) and Halomonas beimenensis NTU-107T (96.93 %). The average nucleotide identity and digital DNA– DNA hybridization values between YJPS3-2T and related type strains were 86.9–91.6 % and 32.0–44.8 %. Strain YJPS3-2T was characterized as having Q-9 as the predominant respiratory quinone and the principal fatty acids (>10 %) were C16: 0 (31.4 %), C19: 0 ω8c cyclo (16.3 %), C17: 0 cyclo (11.9 %) and C12: 0 3-OH (10.4 %). The polar lipids consisted of phosphatidylcho-line, diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The DNA G+C content of strain YJPS3-2T is 68.1mol %. Based on the polyphasic taxonomic evidence presented in this study, YJPS3-2T should be classified as representing a novel species within the genus Halmonas, for which name Halomonas getboli is proposed, with the type strain YJPS3-2T (= KCTC 92124T =KACC 22561T =JCM 35085T).

AB - A novel Gram-stain-negative, rod-shaped, cream-coloured, motile, halotolerant bacterium, designated as YJPS3-2T, was isolated from saltern sediment of the Yellow sea in Yongyu-do, Republic of Korea. Strain YJPS3-2T grew at pH 5.0–10.0 (optimum, pH 7.0), 4–40 °C (optimum, 30 °C) and with 1–15% (w/v) NaCl (optimum 3 %). The 16S rRNA gene sequence analysis indicated that strain YJPS3-2T was closely related to those of Halomonas halophila F5-7T (98.75 %), Halomonas salina F8-11T (98.74 %), Halomonas smyrnensis AAD6T (98.66 %), Halomonas organivorans G-16.1T (98.34 %), Halomonas kore-ensis SS20T (97.98 %) and Halomonas beimenensis NTU-107T (96.93 %). The average nucleotide identity and digital DNA– DNA hybridization values between YJPS3-2T and related type strains were 86.9–91.6 % and 32.0–44.8 %. Strain YJPS3-2T was characterized as having Q-9 as the predominant respiratory quinone and the principal fatty acids (>10 %) were C16: 0 (31.4 %), C19: 0 ω8c cyclo (16.3 %), C17: 0 cyclo (11.9 %) and C12: 0 3-OH (10.4 %). The polar lipids consisted of phosphatidylcho-line, diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The DNA G+C content of strain YJPS3-2T is 68.1mol %. Based on the polyphasic taxonomic evidence presented in this study, YJPS3-2T should be classified as representing a novel species within the genus Halmonas, for which name Halomonas getboli is proposed, with the type strain YJPS3-2T (= KCTC 92124T =KACC 22561T =JCM 35085T).

KW - Getbol

KW - Halomonas

KW - halophile

KW - halotolerant bacteria

KW - marine bacteria

UR - http://www.scopus.com/inward/record.url?scp=85144030022&partnerID=8YFLogxK

U2 - 10.1099/ijsem.0.005634

DO - 10.1099/ijsem.0.005634

M3 - Article

C2 - 36748432

AN - SCOPUS:85144030022

SN - 1466-5026

VL - 72

JO - International Journal of Systematic and Evolutionary Microbiology

JF - International Journal of Systematic and Evolutionary Microbiology

IS - 12

M1 - 005634

ER -

Halomonas getboli sp. nov., a halotolerant bacteria isolated from a salt flat

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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