Haloxyfop-P-methyl induces developmental defects in zebrafish embryos through oxidative stress and anti-vasculogenesis

Sunwoo Park, Jin Young Lee, Hahyun Park, Gwonhwa Song, Whasun Lim

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)


Haloxyfop-P-methyl, an aryloxyphenoxypropionate herbicide, is widely used to eliminate unwanted plants by inhibiting lipid synthesis and inducing oxidative stress. Since haloxyfop-P-methyl targets are limited within plants, few negative side effects on non-target crops have been reported. However, dissolved haloxyfop-P-methyl in rain or groundwater contaminates aquatic environments and affects marine ecosystems. In the present study, treatment with haloxyfop-P-methyl for 48 h induced developmental deficiencies in the eyes and bodies of the zebrafish embryos as a whole and was also linked to increases in the incidence of pericardial edema. Additionally, haloxyfop-P-methyl treatment decreased hatching ratio, embryo viability, and heart rate, while simultaneously increasing the expression levels of apoptotic and inflammatory genes. Moreover, haloxyfop-P-methyl hampered vasculogenesis in the embryos through down-regulation of functional genes, and disruption of vessel formation caused neurodegeneration in the olig2-positive notochord. Collectively, this study newly discovered the oxidative stress-related toxic mechanism of haloxyfop-P-methyl during embryonic development through anti-vasculogenesis, which suppresses neurogenesis of the notochord. This toxicity assessment of haloxyfop-P-methyl on embryogenesis may contribute to establishment of safety profiling of herbicide and to support hazard control in aquatic environment.

Original languageEnglish
Article number108761
JournalComparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology
Publication statusPublished - 2020 Jul

Bibliographical note

Publisher Copyright:
© 2020 Elsevier Inc.


  • Haloxyfop-P-methyl
  • Neurotoxicity
  • Oxidative stress
  • Vasculogenesis
  • Zebrafish

ASJC Scopus subject areas

  • Aquatic Science
  • Animal Science and Zoology
  • Biochemistry
  • Physiology
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Cell Biology


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