TY - JOUR
T1 - Hemodynamic correlation imaging of the mouse brain for application in unilateral neurodegenerative diseases
AU - Paik, Seung Ho
AU - Erdogan, Sedef
AU - Phillips V, Zephaniah
AU - Kim, Youngkyu
AU - Song, Kang Il
AU - Park, Sunghee Estelle
AU - Choi, Youngwoon
AU - Youn, Inchan
AU - Kim, Beop Min
N1 - Funding Information:
Original Technology Research Program for Brain Science through the NRF (National Research Foundation of Korea) funded by Ministry of Science ICT and Future Planning (2015M3C7A1029034); Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI) funded by the Ministry of Health & Welfare, Republic of Korea (HI17C1501).
Funding Information:
Original Technology Research Program for Brain Science through the NRF (National Research Foundation of Korea) funded by Ministry of Science ICT and Future Planning (2015M3C7A1029034); Korea Health Technology R&D Project through the Korea Health Industry Development Institute(KHIDI) funded by the Ministry of Health & Welfare, Republic of Korea (HI17C1501).
Publisher Copyright:
© 2019 Optical Society of America under the terms of the OSA Open Access Publishing.
PY - 2019/4/1
Y1 - 2019/4/1
N2 - We developed a single-camera two-channel hemodynamic imaging system that uses near-infrared light to monitor the mouse brain in vivo with an exposed, un-thinned, and intact skull to explore the effect of Parkinson’s disease on the resting state functional connectivity of the brain. To demonstrate our system’s ability to monitor cerebral hemodynamics, we first performed direct electrical stimulation of an anesthetized healthy mouse brain and detected hemodynamic changes localized to the stimulated area. Subsequently, we developed a unilaterally lesioned 6-hydroxydopamine (hemi-parkinsonian) mouse model and detected the differences in functional connectivity between the normal and hemi-parkinsonian mouse brains by comparing the hemispheric hemodynamic correlations during the resting state. Seed-based correlation for the oxy-hemoglobin channel from the left and right hemispheres of healthy mice was much higher and more symmetric than in hemi-parkinsonian mice. Through a k-means clustering of the hemodynamic signals, the healthy mouse brains were segmented according to brain region, but the hemi-parkinsonian mice did not show a similar segmentation. Overall, this study highlights the development of a spatial multiplexing hemodynamic imaging system that reveals the resting state hemodynamic connectivity in healthy and hemi-parkinsonian mice.
AB - We developed a single-camera two-channel hemodynamic imaging system that uses near-infrared light to monitor the mouse brain in vivo with an exposed, un-thinned, and intact skull to explore the effect of Parkinson’s disease on the resting state functional connectivity of the brain. To demonstrate our system’s ability to monitor cerebral hemodynamics, we first performed direct electrical stimulation of an anesthetized healthy mouse brain and detected hemodynamic changes localized to the stimulated area. Subsequently, we developed a unilaterally lesioned 6-hydroxydopamine (hemi-parkinsonian) mouse model and detected the differences in functional connectivity between the normal and hemi-parkinsonian mouse brains by comparing the hemispheric hemodynamic correlations during the resting state. Seed-based correlation for the oxy-hemoglobin channel from the left and right hemispheres of healthy mice was much higher and more symmetric than in hemi-parkinsonian mice. Through a k-means clustering of the hemodynamic signals, the healthy mouse brains were segmented according to brain region, but the hemi-parkinsonian mice did not show a similar segmentation. Overall, this study highlights the development of a spatial multiplexing hemodynamic imaging system that reveals the resting state hemodynamic connectivity in healthy and hemi-parkinsonian mice.
UR - http://www.scopus.com/inward/record.url?scp=85064462406&partnerID=8YFLogxK
U2 - 10.1364/BOE.10.001736
DO - 10.1364/BOE.10.001736
M3 - Article
AN - SCOPUS:85064462406
SN - 2156-7085
VL - 10
SP - 1736
EP - 1749
JO - Biomedical Optics Express
JF - Biomedical Optics Express
IS - 4
M1 - #349727
ER -