Abstract
Star-shaped PLA-heparin (sPLA-Hep) was prepared by coupling heparin to the star-shaped PLA (sPLA) reaction using carbonyldiimidazole (CDI) chemistry. Hydroxyl groups of sPLA were activated by CDI for the reaction with the remained amino-tenninal groups of heparin. The surface heparin content of sPLA-Hep was measured to be 1.43 μg/cm2. sPLA-Hep-coated surface has shown higher hydrophilicity than control surface. The clotting time of sPLA-Hep measured by activated partial thromboplastin time (APTT) was significantly prolonged when compared with sPLA and linear PLA-Hep. sPLA-Hep surface demonstrated lower protein adsorption and platelet adhesion than control sPLA surface. In addition, fibroblast culture on the sPLA-Hep surface showed the enhanced cell spreading area compared with the sPLA surface. Obtained results suggest that the incorporation of heparin to sPLA is effective in curtailing the surface induced-thrombosis and in manipulating the cell interaction. sPLA-Hep could be applied as blood/tissue compatible biodegradable materials for implantable medical devices and tissue engineering.
| Original language | English |
|---|---|
| Pages (from-to) | 842-848 |
| Number of pages | 7 |
| Journal | Journal of Biomedical Materials Research - Part A |
| Volume | 86 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2008 Sept 1 |
Keywords
- Blood compatibility
- Heparin
- Polylactide
- Tissue compatibility
ASJC Scopus subject areas
- Ceramics and Composites
- Biomaterials
- Biomedical Engineering
- Metals and Alloys