Heparin-immobilized hydroxyapatite nanoparticles as a lactoferrin delivery system for improving osteogenic differentiation of adipose-derived stem cells

Sung Eun Kim; Deok Won Lee; Young Pil Yun; Kyu Sik Shim; Daniel I. Jeon; Jin Kyu Rhee; Hak Jun Kim; Kyeongsoon Park

doi:10.1088/1748-6041/11/2/025004

Heparin-immobilized hydroxyapatite nanoparticles as a lactoferrin delivery system for improving osteogenic differentiation of adipose-derived stem cells

Sung Eun Kim, Deok Won Lee, Young Pil Yun, Kyu Sik Shim, Daniel I. Jeon, Jin Kyu Rhee, Hak Jun Kim, Kyeongsoon Park

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

The aim of this study is to fabricate lactoferrin (LF)-carrying hydroxyapatite nanoparticles (HAp NPs) to enhance osteogenic differentiation of rabbit adipose-derived stem cells (rADSCs). HAp NPs were modified with heparin-dopamine (Hep-DOPA) (Hep-HAp) and further immobilized with LF (LF/Hep-HAp). Heparin immobilization on HAp NPs prevented aggregation of HAp NPs in aqueous solution and prolonged the release of LF from LF/Hep-HAp NPs. In vitro studies of rADSCs have demonstrated that LF-Hep/HAp NPs significantly increase alkaline phosphatase (ALP) activity, calcium deposition, and both mRNA expression of osteocalcin (OCN) and osteopontin (OPN) in comparison with HAp and Hep-HAp NPs. These results suggest that LF/Hep-HAp NPs can effectively induce osteogenic differentiation of rADSCs.

Original language	English
Article number	025004
Journal	Biomedical Materials (Bristol)
Volume	11
Issue number	2
DOIs	https://doi.org/10.1088/1748-6041/11/2/025004
Publication status	Published - 2016 Mar 10

Bibliographical note

Funding Information:
This study was supported by a grant provided by the Korea Health Technology R&D Project, Ministry of Health and Welfare, Republic of Korea (HI11C0388 and HI13C1501).

Publisher Copyright:
© 2016 IOP Publishing Ltd.

Keywords

adipose-derived stem cells
heparin
hydroxyapatite nanoparticles
lactoferrin delivery
osteogenic differentiation

ASJC Scopus subject areas

Bioengineering
Biomaterials
Biomedical Engineering

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10.1088/1748-6041/11/2/025004

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title = "Heparin-immobilized hydroxyapatite nanoparticles as a lactoferrin delivery system for improving osteogenic differentiation of adipose-derived stem cells",

abstract = "The aim of this study is to fabricate lactoferrin (LF)-carrying hydroxyapatite nanoparticles (HAp NPs) to enhance osteogenic differentiation of rabbit adipose-derived stem cells (rADSCs). HAp NPs were modified with heparin-dopamine (Hep-DOPA) (Hep-HAp) and further immobilized with LF (LF/Hep-HAp). Heparin immobilization on HAp NPs prevented aggregation of HAp NPs in aqueous solution and prolonged the release of LF from LF/Hep-HAp NPs. In vitro studies of rADSCs have demonstrated that LF-Hep/HAp NPs significantly increase alkaline phosphatase (ALP) activity, calcium deposition, and both mRNA expression of osteocalcin (OCN) and osteopontin (OPN) in comparison with HAp and Hep-HAp NPs. These results suggest that LF/Hep-HAp NPs can effectively induce osteogenic differentiation of rADSCs.",

keywords = "adipose-derived stem cells, heparin, hydroxyapatite nanoparticles, lactoferrin delivery, osteogenic differentiation",

author = "Kim, {Sung Eun} and Lee, {Deok Won} and Yun, {Young Pil} and Shim, {Kyu Sik} and Jeon, {Daniel I.} and Rhee, {Jin Kyu} and Kim, {Hak Jun} and Kyeongsoon Park",

note = "Funding Information: This study was supported by a grant provided by the Korea Health Technology R&D Project, Ministry of Health and Welfare, Republic of Korea (HI11C0388 and HI13C1501). Publisher Copyright: {\textcopyright} 2016 IOP Publishing Ltd.",

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doi = "10.1088/1748-6041/11/2/025004",

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T1 - Heparin-immobilized hydroxyapatite nanoparticles as a lactoferrin delivery system for improving osteogenic differentiation of adipose-derived stem cells

AU - Kim, Sung Eun

AU - Lee, Deok Won

AU - Yun, Young Pil

AU - Shim, Kyu Sik

AU - Jeon, Daniel I.

AU - Rhee, Jin Kyu

AU - Kim, Hak Jun

AU - Park, Kyeongsoon

N1 - Funding Information: This study was supported by a grant provided by the Korea Health Technology R&D Project, Ministry of Health and Welfare, Republic of Korea (HI11C0388 and HI13C1501). Publisher Copyright: © 2016 IOP Publishing Ltd.

PY - 2016/3/10

Y1 - 2016/3/10

N2 - The aim of this study is to fabricate lactoferrin (LF)-carrying hydroxyapatite nanoparticles (HAp NPs) to enhance osteogenic differentiation of rabbit adipose-derived stem cells (rADSCs). HAp NPs were modified with heparin-dopamine (Hep-DOPA) (Hep-HAp) and further immobilized with LF (LF/Hep-HAp). Heparin immobilization on HAp NPs prevented aggregation of HAp NPs in aqueous solution and prolonged the release of LF from LF/Hep-HAp NPs. In vitro studies of rADSCs have demonstrated that LF-Hep/HAp NPs significantly increase alkaline phosphatase (ALP) activity, calcium deposition, and both mRNA expression of osteocalcin (OCN) and osteopontin (OPN) in comparison with HAp and Hep-HAp NPs. These results suggest that LF/Hep-HAp NPs can effectively induce osteogenic differentiation of rADSCs.

AB - The aim of this study is to fabricate lactoferrin (LF)-carrying hydroxyapatite nanoparticles (HAp NPs) to enhance osteogenic differentiation of rabbit adipose-derived stem cells (rADSCs). HAp NPs were modified with heparin-dopamine (Hep-DOPA) (Hep-HAp) and further immobilized with LF (LF/Hep-HAp). Heparin immobilization on HAp NPs prevented aggregation of HAp NPs in aqueous solution and prolonged the release of LF from LF/Hep-HAp NPs. In vitro studies of rADSCs have demonstrated that LF-Hep/HAp NPs significantly increase alkaline phosphatase (ALP) activity, calcium deposition, and both mRNA expression of osteocalcin (OCN) and osteopontin (OPN) in comparison with HAp and Hep-HAp NPs. These results suggest that LF/Hep-HAp NPs can effectively induce osteogenic differentiation of rADSCs.

KW - adipose-derived stem cells

KW - heparin

KW - hydroxyapatite nanoparticles

KW - lactoferrin delivery

KW - osteogenic differentiation

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Heparin-immobilized hydroxyapatite nanoparticles as a lactoferrin delivery system for improving osteogenic differentiation of adipose-derived stem cells

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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