Abstract
The aim of this study is to fabricate lactoferrin (LF)-carrying hydroxyapatite nanoparticles (HAp NPs) to enhance osteogenic differentiation of rabbit adipose-derived stem cells (rADSCs). HAp NPs were modified with heparin-dopamine (Hep-DOPA) (Hep-HAp) and further immobilized with LF (LF/Hep-HAp). Heparin immobilization on HAp NPs prevented aggregation of HAp NPs in aqueous solution and prolonged the release of LF from LF/Hep-HAp NPs. In vitro studies of rADSCs have demonstrated that LF-Hep/HAp NPs significantly increase alkaline phosphatase (ALP) activity, calcium deposition, and both mRNA expression of osteocalcin (OCN) and osteopontin (OPN) in comparison with HAp and Hep-HAp NPs. These results suggest that LF/Hep-HAp NPs can effectively induce osteogenic differentiation of rADSCs.
Original language | English |
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Article number | 025004 |
Journal | Biomedical Materials (Bristol) |
Volume | 11 |
Issue number | 2 |
DOIs | |
Publication status | Published - 2016 Mar 10 |
Bibliographical note
Funding Information:This study was supported by a grant provided by the Korea Health Technology R&D Project, Ministry of Health and Welfare, Republic of Korea (HI11C0388 and HI13C1501).
Publisher Copyright:
© 2016 IOP Publishing Ltd.
Keywords
- adipose-derived stem cells
- heparin
- hydroxyapatite nanoparticles
- lactoferrin delivery
- osteogenic differentiation
ASJC Scopus subject areas
- Bioengineering
- Biomaterials
- Biomedical Engineering