Hepatoprotective activity of water extracts from chaga medicinal mushroom, inonotus obliquus (Higher basidiomycetes) against tert-butyl hydroperoxide–induced oxidative liver injury in primary cultured rat hepatocytes

Ki Bae Hong; Dong Ouk Noh; Yooheon Park; Hyung Joo Suh

Hepatoprotective activity of water extracts from chaga medicinal mushroom, inonotus obliquus (Higher basidiomycetes) against tert-butyl hydroperoxide–induced oxidative liver injury in primary cultured rat hepatocytes

Ki Bae Hong, Dong Ouk Noh, Yooheon Park, Hyung Joo Suh

Department of Public Health Sciences

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

We examined the hepatoprotective activity of Inonotus obliquus water extract (IO-W) against tertbutyl hydroperoxide (t-BHP)–induced oxidative liver injury in the primary cultured rat hepatocyte. The 50% radical scavenging concentrations (SC₅₀s) of IO-W for radical-scavenging activity against 2,2′-azino-bis-(3-ethylbenzothiazoline- 6-sulfonic acid) (ABTS) and 1,1-diphenyl-2-picryl-hydrazyl (DPPH) were 5.19 mg/mL and 0.39 mg/mL, respectively. IO-W pretreatment to the primary cultured hepatocytes significantly (p100 μg/ mL). In conclusion, this study demonstrates that IO-W exhibited hepatoprotective activity against t-BHP–induced oxidative liver injury in the primary cultured hepatocyte probably via its abilities of quenching free radicals, inhibiting the leakage of ALT, AST, and LDH, and decreasing MDA formation.

Original language	English
Pages (from-to)	1069-1076
Number of pages	8
Journal	International Journal of Medicinal Mushrooms
Volume	17
Issue number	11
Publication status	Published - 2015

Keywords

Chaga mushroom
Hepatocyte
Inonotus obliquus
Liver injury
Medicinal mushrooms
Primary culture
Tert-butyl hydroperoxide

ASJC Scopus subject areas

Applied Microbiology and Biotechnology
Drug Discovery
Pharmacology

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Hepatoprotective activity of water extracts from chaga medicinal mushroom, inonotus obliquus (Higher basidiomycetes) against tert-butyl hydroperoxide–induced oxidative liver injury in primary cultured rat hepatocytes. / Hong, Ki Bae; Noh, Dong Ouk; Park, Yooheon et al.
In: International Journal of Medicinal Mushrooms, Vol. 17, No. 11, 2015, p. 1069-1076.

Research output: Contribution to journal › Article › peer-review

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title = "Hepatoprotective activity of water extracts from chaga medicinal mushroom, inonotus obliquus (Higher basidiomycetes) against tert-butyl hydroperoxide–induced oxidative liver injury in primary cultured rat hepatocytes",

abstract = "We examined the hepatoprotective activity of Inonotus obliquus water extract (IO-W) against tertbutyl hydroperoxide (t-BHP)–induced oxidative liver injury in the primary cultured rat hepatocyte. The 50% radical scavenging concentrations (SC50s) of IO-W for radical-scavenging activity against 2,2′-azino-bis-(3-ethylbenzothiazoline- 6-sulfonic acid) (ABTS) and 1,1-diphenyl-2-picryl-hydrazyl (DPPH) were 5.19 mg/mL and 0.39 mg/mL, respectively. IO-W pretreatment to the primary cultured hepatocytes significantly (p100 μg/ mL). In conclusion, this study demonstrates that IO-W exhibited hepatoprotective activity against t-BHP–induced oxidative liver injury in the primary cultured hepatocyte probably via its abilities of quenching free radicals, inhibiting the leakage of ALT, AST, and LDH, and decreasing MDA formation.",

keywords = "Chaga mushroom, Hepatocyte, Inonotus obliquus, Liver injury, Medicinal mushrooms, Primary culture, Tert-butyl hydroperoxide",

author = "Hong, {Ki Bae} and Noh, {Dong Ouk} and Yooheon Park and Suh, {Hyung Joo}",

year = "2015",

language = "English",

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AU - Hong, Ki Bae

AU - Noh, Dong Ouk

AU - Park, Yooheon

AU - Suh, Hyung Joo

PY - 2015

Y1 - 2015

N2 - We examined the hepatoprotective activity of Inonotus obliquus water extract (IO-W) against tertbutyl hydroperoxide (t-BHP)–induced oxidative liver injury in the primary cultured rat hepatocyte. The 50% radical scavenging concentrations (SC50s) of IO-W for radical-scavenging activity against 2,2′-azino-bis-(3-ethylbenzothiazoline- 6-sulfonic acid) (ABTS) and 1,1-diphenyl-2-picryl-hydrazyl (DPPH) were 5.19 mg/mL and 0.39 mg/mL, respectively. IO-W pretreatment to the primary cultured hepatocytes significantly (p100 μg/ mL). In conclusion, this study demonstrates that IO-W exhibited hepatoprotective activity against t-BHP–induced oxidative liver injury in the primary cultured hepatocyte probably via its abilities of quenching free radicals, inhibiting the leakage of ALT, AST, and LDH, and decreasing MDA formation.

AB - We examined the hepatoprotective activity of Inonotus obliquus water extract (IO-W) against tertbutyl hydroperoxide (t-BHP)–induced oxidative liver injury in the primary cultured rat hepatocyte. The 50% radical scavenging concentrations (SC50s) of IO-W for radical-scavenging activity against 2,2′-azino-bis-(3-ethylbenzothiazoline- 6-sulfonic acid) (ABTS) and 1,1-diphenyl-2-picryl-hydrazyl (DPPH) were 5.19 mg/mL and 0.39 mg/mL, respectively. IO-W pretreatment to the primary cultured hepatocytes significantly (p100 μg/ mL). In conclusion, this study demonstrates that IO-W exhibited hepatoprotective activity against t-BHP–induced oxidative liver injury in the primary cultured hepatocyte probably via its abilities of quenching free radicals, inhibiting the leakage of ALT, AST, and LDH, and decreasing MDA formation.

KW - Chaga mushroom

KW - Hepatocyte

KW - Inonotus obliquus

KW - Liver injury

KW - Medicinal mushrooms

KW - Primary culture

KW - Tert-butyl hydroperoxide

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M3 - Article

C2 - 26853962

AN - SCOPUS:84955438374

SN - 1521-9437

VL - 17

SP - 1069

EP - 1076

JO - International Journal of Medicinal Mushrooms

JF - International Journal of Medicinal Mushrooms

IS - 11

ER -

Hepatoprotective activity of water extracts from chaga medicinal mushroom, inonotus obliquus (Higher basidiomycetes) against tert-butyl hydroperoxide–induced oxidative liver injury in primary cultured rat hepatocytes

Abstract

Keywords

ASJC Scopus subject areas

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