Major histocompatibility complex (MHC) class II molecules, which are recognized for their primary function of presenting an antigen to the T cell receptor, are involved in various signaling pathways in B cell activation. We identified heterogeneous nuclear ribonucleoprotein (hnRNP) A2B1 as an MHC class II molecule-associated protein involved in MHC class II-mediated signal transduction in lipopolysaccharide (LPS)-stimulated 38B9 B cells. Although the function of hnRNP A2B1 in the nucleus is primarily known, the level of hnRNP A2B1 in the cytoplasm was increased in LPS-stimulated 38B9 cells, while it was not detected in the cytoplasm of non-treated 38B9 cells. The silencing of hnRNP A2B1 expression using siRNA disturbed B cell maturation by regulation of mitogen-activated protein kinase signaling, NF-κB activation, and protein kinase B activation. These results suggest that hnRNP A2B1 is associated with MHC class II molecules and is involved in B cell activation signaling pathways in LPS-stimulated 38B9 cells.
Bibliographical noteFunding Information:
This study was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Korean Ministry of Science, ICT & Future Planning (2016R1A2B2010096). Dr. Yong-Suk Jang was supported by the Research Base Construction Fund Support Program funded by Chonbuk National University in 2017. Mr. Jisang Park was supported by the BK21 PLUS program in the Department of Bioactive Material Sciences. Flow cytometry and confocal laser scanning microscopy were performed using the instruments installed in the Center for University-Wide Research Facilities at Chonbuk National University.
© 2017. The Korean Association of Immunologists.
- B cell
- Heterogeneous-nuclear ribonucleoproteins
- MHC class II molecule
- Signal transduction
ASJC Scopus subject areas
- Immunology and Allergy
- Infectious Diseases