Heterologous expression of endo-1,4-beta-xylanaseC from Phanerochaete chrysosporium in Pichia pastoris

Nguyen Duc Huy, Seung Wook Kim, Seung Moon Park

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

The cDNA of endo-1,4-β-xylanaseC, isolated from Phanerochaete chrysosporium, was expressed in Pichia pastoris, under the control of the alcohol oxidase I promoter. Using either the intrinsic leader peptide of xylanaseC or the α-factor signal peptide of Saccharomyces cerevisiae, xylanaseC is efficiently secreted into the medium, at a maximum concentration of 2500U.l-1.

Original languageEnglish
Pages (from-to)654-657
Number of pages4
JournalJournal of Bioscience and Bioengineering
Volume111
Issue number6
DOIs
Publication statusPublished - 2011 Jun

Bibliographical note

Funding Information:
This research was supported by Technology Development Program for Agriculture and Forestry, Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea . We thank the Center for University-Wide Research Facility and Research Institute of Bioindustry at Chonbuk National University for kindly providing the facilities for this research.

Keywords

  • Glycoside hydrolase
  • Phanerochaete chrysosporium
  • Pichia pastoris
  • Xylan
  • Xylanase

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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