Heterologous expression of endo-1,4-beta-xylanaseC from Phanerochaete chrysosporium in Pichia pastoris

Nguyen Duc Huy; Seung Wook Kim; Seung Moon Park

doi:10.1016/j.jbiosc.2011.02.010

Heterologous expression of endo-1,4-beta-xylanaseC from Phanerochaete chrysosporium in Pichia pastoris

Nguyen Duc Huy
, Seung Wook Kim
, Seung Moon Park^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

The cDNA of endo-1,4-β-xylanaseC, isolated from Phanerochaete chrysosporium, was expressed in Pichia pastoris, under the control of the alcohol oxidase I promoter. Using either the intrinsic leader peptide of xylanaseC or the α-factor signal peptide of Saccharomyces cerevisiae, xylanaseC is efficiently secreted into the medium, at a maximum concentration of 2500U.l^-1.

Original language	English
Pages (from-to)	654-657
Number of pages	4
Journal	Journal of Bioscience and Bioengineering
Volume	111
Issue number	6
DOIs	https://doi.org/10.1016/j.jbiosc.2011.02.010
Publication status	Published - 2011 Jun

Bibliographical note

Funding Information:
This research was supported by Technology Development Program for Agriculture and Forestry, Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea . We thank the Center for University-Wide Research Facility and Research Institute of Bioindustry at Chonbuk National University for kindly providing the facilities for this research.

Keywords

Glycoside hydrolase
Phanerochaete chrysosporium
Pichia pastoris
Xylan
Xylanase

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1016/j.jbiosc.2011.02.010

Cite this

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title = "Heterologous expression of endo-1,4-beta-xylanaseC from Phanerochaete chrysosporium in Pichia pastoris",

abstract = "The cDNA of endo-1,4-β-xylanaseC, isolated from Phanerochaete chrysosporium, was expressed in Pichia pastoris, under the control of the alcohol oxidase I promoter. Using either the intrinsic leader peptide of xylanaseC or the α-factor signal peptide of Saccharomyces cerevisiae, xylanaseC is efficiently secreted into the medium, at a maximum concentration of 2500U.l-1.",

keywords = "Glycoside hydrolase, Phanerochaete chrysosporium, Pichia pastoris, Xylan, Xylanase",

author = "Huy, \{Nguyen Duc\} and Kim, \{Seung Wook\} and Park, \{Seung Moon\}",

note = "Funding Information: This research was supported by Technology Development Program for Agriculture and Forestry, Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea . We thank the Center for University-Wide Research Facility and Research Institute of Bioindustry at Chonbuk National University for kindly providing the facilities for this research.",

year = "2011",

month = jun,

doi = "10.1016/j.jbiosc.2011.02.010",

language = "English",

volume = "111",

pages = "654--657",

journal = "Journal of Bioscience and Bioengineering",

issn = "1389-1723",

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AU - Huy, Nguyen Duc

AU - Kim, Seung Wook

AU - Park, Seung Moon

N1 - Funding Information: This research was supported by Technology Development Program for Agriculture and Forestry, Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea . We thank the Center for University-Wide Research Facility and Research Institute of Bioindustry at Chonbuk National University for kindly providing the facilities for this research.

PY - 2011/6

Y1 - 2011/6

N2 - The cDNA of endo-1,4-β-xylanaseC, isolated from Phanerochaete chrysosporium, was expressed in Pichia pastoris, under the control of the alcohol oxidase I promoter. Using either the intrinsic leader peptide of xylanaseC or the α-factor signal peptide of Saccharomyces cerevisiae, xylanaseC is efficiently secreted into the medium, at a maximum concentration of 2500U.l-1.

AB - The cDNA of endo-1,4-β-xylanaseC, isolated from Phanerochaete chrysosporium, was expressed in Pichia pastoris, under the control of the alcohol oxidase I promoter. Using either the intrinsic leader peptide of xylanaseC or the α-factor signal peptide of Saccharomyces cerevisiae, xylanaseC is efficiently secreted into the medium, at a maximum concentration of 2500U.l-1.

KW - Glycoside hydrolase

KW - Phanerochaete chrysosporium

KW - Pichia pastoris

KW - Xylan

KW - Xylanase

UR - https://www.scopus.com/pages/publications/79957616856

U2 - 10.1016/j.jbiosc.2011.02.010

DO - 10.1016/j.jbiosc.2011.02.010

M3 - Article

C2 - 21393056

AN - SCOPUS:79957616856

SN - 1389-1723

VL - 111

SP - 654

EP - 657

JO - Journal of Bioscience and Bioengineering

JF - Journal of Bioscience and Bioengineering

IS - 6

ER -

Heterologous expression of endo-1,4-beta-xylanaseC from Phanerochaete chrysosporium in Pichia pastoris

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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