Heterologous expression of the kanamycin biosynthetic gene cluster (pSKC2) in Streptomyces venezuelae YJ003

Laxmi Prasad Thapa; Tae Jin Oh; Hei Chan Lee; Kwangkyoung Liou; Je Won Park; Yeo Joon Yoon; Jae Kyung Sohng

doi:10.1007/s00253-007-1096-4

Heterologous expression of the kanamycin biosynthetic gene cluster (pSKC2) in Streptomyces venezuelae YJ003

Laxmi Prasad Thapa, Tae Jin Oh, Hei Chan Lee, Kwangkyoung Liou, Je Won Park, Yeo Joon Yoon, Jae Kyung Sohng

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

The pSKC2 cosmid, which has 32 kb and 28 open-reading frames, was isolated from Streptomyces kanamyceticus ATCC12853 as the gene cluster of kanamycin. This gene cluster includes the minimal biosynthetic genes of kanamycin with the resistance and regulatory genes. It was heterologously expressed in Streptomyces venezuelae YJ003, which has the advantage of fast growth, good efficiency of the transformation host, and rapid production of the aminoglycosides antibiotic. The isolated compound was analyzed by electrospray ionization-mass spectrometry, liquid chromatography-mass spectrometry, high-performance liquid chromatography, and tandem mass spectrometry and shows a molecular weight of 485 as kanamycin A.

Original language	English
Pages (from-to)	1357-1364
Number of pages	8
Journal	Applied Microbiology and Biotechnology
Volume	76
Issue number	6
DOIs	https://doi.org/10.1007/s00253-007-1096-4
Publication status	Published - 2007 Oct
Externally published	Yes

Bibliographical note

Funding Information:
Acknowledgments This work was supported by Ministry of Commerce, Industry and Energy (grant no. 10023194) and by a grant (no. 20050401034682) from the BioGreen 21 Program, Rural Development Administration, Republic of Korea.

Keywords

Heterologous expression
Kanamycin
Streptomyces kanamyceticus ATCC 12853
Streptomyces venezuelae YJ003

ASJC Scopus subject areas

Biotechnology
Applied Microbiology and Biotechnology

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10.1007/s00253-007-1096-4

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title = "Heterologous expression of the kanamycin biosynthetic gene cluster (pSKC2) in Streptomyces venezuelae YJ003",

abstract = "The pSKC2 cosmid, which has 32 kb and 28 open-reading frames, was isolated from Streptomyces kanamyceticus ATCC12853 as the gene cluster of kanamycin. This gene cluster includes the minimal biosynthetic genes of kanamycin with the resistance and regulatory genes. It was heterologously expressed in Streptomyces venezuelae YJ003, which has the advantage of fast growth, good efficiency of the transformation host, and rapid production of the aminoglycosides antibiotic. The isolated compound was analyzed by electrospray ionization-mass spectrometry, liquid chromatography-mass spectrometry, high-performance liquid chromatography, and tandem mass spectrometry and shows a molecular weight of 485 as kanamycin A.",

keywords = "Heterologous expression, Kanamycin, Streptomyces kanamyceticus ATCC 12853, Streptomyces venezuelae YJ003",

author = "Thapa, {Laxmi Prasad} and Oh, {Tae Jin} and Lee, {Hei Chan} and Kwangkyoung Liou and Park, {Je Won} and Yoon, {Yeo Joon} and Sohng, {Jae Kyung}",

note = "Funding Information: Acknowledgments This work was supported by Ministry of Commerce, Industry and Energy (grant no. 10023194) and by a grant (no. 20050401034682) from the BioGreen 21 Program, Rural Development Administration, Republic of Korea.",

year = "2007",

month = oct,

doi = "10.1007/s00253-007-1096-4",

language = "English",

volume = "76",

pages = "1357--1364",

journal = "Applied Microbiology and Biotechnology",

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T1 - Heterologous expression of the kanamycin biosynthetic gene cluster (pSKC2) in Streptomyces venezuelae YJ003

AU - Thapa, Laxmi Prasad

AU - Oh, Tae Jin

AU - Lee, Hei Chan

AU - Liou, Kwangkyoung

AU - Park, Je Won

AU - Yoon, Yeo Joon

AU - Sohng, Jae Kyung

N1 - Funding Information: Acknowledgments This work was supported by Ministry of Commerce, Industry and Energy (grant no. 10023194) and by a grant (no. 20050401034682) from the BioGreen 21 Program, Rural Development Administration, Republic of Korea.

PY - 2007/10

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N2 - The pSKC2 cosmid, which has 32 kb and 28 open-reading frames, was isolated from Streptomyces kanamyceticus ATCC12853 as the gene cluster of kanamycin. This gene cluster includes the minimal biosynthetic genes of kanamycin with the resistance and regulatory genes. It was heterologously expressed in Streptomyces venezuelae YJ003, which has the advantage of fast growth, good efficiency of the transformation host, and rapid production of the aminoglycosides antibiotic. The isolated compound was analyzed by electrospray ionization-mass spectrometry, liquid chromatography-mass spectrometry, high-performance liquid chromatography, and tandem mass spectrometry and shows a molecular weight of 485 as kanamycin A.

AB - The pSKC2 cosmid, which has 32 kb and 28 open-reading frames, was isolated from Streptomyces kanamyceticus ATCC12853 as the gene cluster of kanamycin. This gene cluster includes the minimal biosynthetic genes of kanamycin with the resistance and regulatory genes. It was heterologously expressed in Streptomyces venezuelae YJ003, which has the advantage of fast growth, good efficiency of the transformation host, and rapid production of the aminoglycosides antibiotic. The isolated compound was analyzed by electrospray ionization-mass spectrometry, liquid chromatography-mass spectrometry, high-performance liquid chromatography, and tandem mass spectrometry and shows a molecular weight of 485 as kanamycin A.

KW - Heterologous expression

KW - Kanamycin

KW - Streptomyces kanamyceticus ATCC 12853

KW - Streptomyces venezuelae YJ003

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SN - 0175-7598

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JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

IS - 6

ER -

Heterologous expression of the kanamycin biosynthetic gene cluster (pSKC2) in Streptomyces venezuelae YJ003

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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