Hexafluoropropylene Oxide Dimer Acid (Genx) Exposure Induces Apoptosis In Hepg2 Cells

Hee Joon Yoo; Min Cheol Pyo; Yoonjin Park; Bo Yong Kim; Kwang Won Lee

doi:10.1016/j.heliyon.2021.e08272

Hexafluoropropylene Oxide Dimer Acid (Genx) Exposure Induces Apoptosis In Hepg2 Cells

Hee Joon Yoo, Min Cheol Pyo, Yoonjin Park, Bo Yong Kim, Kwang Won Lee

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Hexafluoropropylene Oxide Dimer Acid, Also Known As Genx, Is A Poly-and Perfluoroalkyl Substance (Pfas). Pfass Are Nonvolatile Synthetic Substances That Can Be Readily Disseminated Into The Environment During Processing And Use, Making Them Easy To Implement In The Soil, Drinking Water, And Air. Compared To Other Pfass, Genx Has A Comparatively Short Carbon Chain Length And Is Expected To Have A Lower Tendency To Accumulate In Humans; Therefore, Genx Has Recently Been Used As A Substitute To Other Pfass. However, The Mechanisms Underlying Genx Action And Intoxication In Humans Remains Unclear. In This Study, The Apoptotic Capacity Of Genx In Human Liver Cells Was Investigated. When Representative Human-Derived Liver Cells (Hepg2 Cells) Were Treated With Genx For 12 H, Cell Viability Was Reduced, And Apoptosis Was Greatly Increased. In Addition, Genx Increased The Generation Of Intracellular Reactive Oxygen Species (Ros), Indicating The Induction Of Oxidative Stress In A Dose-Dependent Manner. Genx Treatment Increased The Expression Of Major Apoptosis-Related Genes Relative To The Untreated Control Group.

Original language	English
Article number	e08272
Journal	Heliyon
Volume	7
Issue number	11
DOIs	https://doi.org/10.1016/j.heliyon.2021.e08272
Publication status	Published - 2021 Nov

Bibliographical note

Funding Information:
The Authors Would Like To Express Their Gratitude To The School Of Life Sciences & Biotechnology Of Korea University For Bk21plus, Republic Of Korea, And The Institute Of Biomedical Science & Food Safety, Korea University-Cj Food Safety Hall (Seoul, Republic Of Korea) For Providing The Equipment And Facilities

Funding Information:
This work was supported by Korea University Research Grant (K2009551).

Publisher Copyright:
© 2021 The Authors. Published by Elsevier Ltd.

Keywords

Apoptosis
Hexafluoropropylene oxide dimer acid
Liver cells
Oxidative stress
Poly and perfluoroalkyl substance

ASJC Scopus subject areas

General

Access to Document

10.1016/j.heliyon.2021.e08272

Cite this

@article{1b20925262cc4ed386ba49a2329436c5,

title = "Hexafluoropropylene Oxide Dimer Acid (Genx) Exposure Induces Apoptosis In Hepg2 Cells",

abstract = "Hexafluoropropylene Oxide Dimer Acid, Also Known As Genx, Is A Poly-and Perfluoroalkyl Substance (Pfas). Pfass Are Nonvolatile Synthetic Substances That Can Be Readily Disseminated Into The Environment During Processing And Use, Making Them Easy To Implement In The Soil, Drinking Water, And Air. Compared To Other Pfass, Genx Has A Comparatively Short Carbon Chain Length And Is Expected To Have A Lower Tendency To Accumulate In Humans; Therefore, Genx Has Recently Been Used As A Substitute To Other Pfass. However, The Mechanisms Underlying Genx Action And Intoxication In Humans Remains Unclear. In This Study, The Apoptotic Capacity Of Genx In Human Liver Cells Was Investigated. When Representative Human-Derived Liver Cells (Hepg2 Cells) Were Treated With Genx For 12 H, Cell Viability Was Reduced, And Apoptosis Was Greatly Increased. In Addition, Genx Increased The Generation Of Intracellular Reactive Oxygen Species (Ros), Indicating The Induction Of Oxidative Stress In A Dose-Dependent Manner. Genx Treatment Increased The Expression Of Major Apoptosis-Related Genes Relative To The Untreated Control Group.",

keywords = "Apoptosis, Hexafluoropropylene oxide dimer acid, Liver cells, Oxidative stress, Poly and perfluoroalkyl substance",

author = "Yoo, {Hee Joon} and Pyo, {Min Cheol} and Yoonjin Park and Kim, {Bo Yong} and Lee, {Kwang Won}",

note = "Funding Information: The Authors Would Like To Express Their Gratitude To The School Of Life Sciences & Biotechnology Of Korea University For Bk21plus, Republic Of Korea, And The Institute Of Biomedical Science & Food Safety, Korea University-Cj Food Safety Hall (Seoul, Republic Of Korea) For Providing The Equipment And Facilities Funding Information: This work was supported by Korea University Research Grant (K2009551). Publisher Copyright: {\textcopyright} 2021 The Authors. Published by Elsevier Ltd.",

year = "2021",

month = nov,

doi = "10.1016/j.heliyon.2021.e08272",

language = "English",

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issn = "2405-8440",

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AU - Yoo, Hee Joon

AU - Pyo, Min Cheol

AU - Park, Yoonjin

AU - Kim, Bo Yong

AU - Lee, Kwang Won

N1 - Funding Information: The Authors Would Like To Express Their Gratitude To The School Of Life Sciences & Biotechnology Of Korea University For Bk21plus, Republic Of Korea, And The Institute Of Biomedical Science & Food Safety, Korea University-Cj Food Safety Hall (Seoul, Republic Of Korea) For Providing The Equipment And Facilities Funding Information: This work was supported by Korea University Research Grant (K2009551). Publisher Copyright: © 2021 The Authors. Published by Elsevier Ltd.

PY - 2021/11

Y1 - 2021/11

N2 - Hexafluoropropylene Oxide Dimer Acid, Also Known As Genx, Is A Poly-and Perfluoroalkyl Substance (Pfas). Pfass Are Nonvolatile Synthetic Substances That Can Be Readily Disseminated Into The Environment During Processing And Use, Making Them Easy To Implement In The Soil, Drinking Water, And Air. Compared To Other Pfass, Genx Has A Comparatively Short Carbon Chain Length And Is Expected To Have A Lower Tendency To Accumulate In Humans; Therefore, Genx Has Recently Been Used As A Substitute To Other Pfass. However, The Mechanisms Underlying Genx Action And Intoxication In Humans Remains Unclear. In This Study, The Apoptotic Capacity Of Genx In Human Liver Cells Was Investigated. When Representative Human-Derived Liver Cells (Hepg2 Cells) Were Treated With Genx For 12 H, Cell Viability Was Reduced, And Apoptosis Was Greatly Increased. In Addition, Genx Increased The Generation Of Intracellular Reactive Oxygen Species (Ros), Indicating The Induction Of Oxidative Stress In A Dose-Dependent Manner. Genx Treatment Increased The Expression Of Major Apoptosis-Related Genes Relative To The Untreated Control Group.

AB - Hexafluoropropylene Oxide Dimer Acid, Also Known As Genx, Is A Poly-and Perfluoroalkyl Substance (Pfas). Pfass Are Nonvolatile Synthetic Substances That Can Be Readily Disseminated Into The Environment During Processing And Use, Making Them Easy To Implement In The Soil, Drinking Water, And Air. Compared To Other Pfass, Genx Has A Comparatively Short Carbon Chain Length And Is Expected To Have A Lower Tendency To Accumulate In Humans; Therefore, Genx Has Recently Been Used As A Substitute To Other Pfass. However, The Mechanisms Underlying Genx Action And Intoxication In Humans Remains Unclear. In This Study, The Apoptotic Capacity Of Genx In Human Liver Cells Was Investigated. When Representative Human-Derived Liver Cells (Hepg2 Cells) Were Treated With Genx For 12 H, Cell Viability Was Reduced, And Apoptosis Was Greatly Increased. In Addition, Genx Increased The Generation Of Intracellular Reactive Oxygen Species (Ros), Indicating The Induction Of Oxidative Stress In A Dose-Dependent Manner. Genx Treatment Increased The Expression Of Major Apoptosis-Related Genes Relative To The Untreated Control Group.

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Hexafluoropropylene Oxide Dimer Acid (Genx) Exposure Induces Apoptosis In Hepg2 Cells

Abstract

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Keywords

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