TY - JOUR
T1 - Hexafluoropropylene Oxide Dimer Acid (Genx) Exposure Induces Apoptosis In Hepg2 Cells
AU - Yoo, Hee Joon
AU - Pyo, Min Cheol
AU - Park, Yoonjin
AU - Kim, Bo Yong
AU - Lee, Kwang Won
N1 - Funding Information:
The Authors Would Like To Express Their Gratitude To The School Of Life Sciences & Biotechnology Of Korea University For Bk21plus, Republic Of Korea, And The Institute Of Biomedical Science & Food Safety, Korea University-Cj Food Safety Hall (Seoul, Republic Of Korea) For Providing The Equipment And Facilities
Funding Information:
This work was supported by Korea University Research Grant (K2009551).
Publisher Copyright:
© 2021 The Authors. Published by Elsevier Ltd.
PY - 2021/11
Y1 - 2021/11
N2 - Hexafluoropropylene Oxide Dimer Acid, Also Known As Genx, Is A Poly-and Perfluoroalkyl Substance (Pfas). Pfass Are Nonvolatile Synthetic Substances That Can Be Readily Disseminated Into The Environment During Processing And Use, Making Them Easy To Implement In The Soil, Drinking Water, And Air. Compared To Other Pfass, Genx Has A Comparatively Short Carbon Chain Length And Is Expected To Have A Lower Tendency To Accumulate In Humans; Therefore, Genx Has Recently Been Used As A Substitute To Other Pfass. However, The Mechanisms Underlying Genx Action And Intoxication In Humans Remains Unclear. In This Study, The Apoptotic Capacity Of Genx In Human Liver Cells Was Investigated. When Representative Human-Derived Liver Cells (Hepg2 Cells) Were Treated With Genx For 12 H, Cell Viability Was Reduced, And Apoptosis Was Greatly Increased. In Addition, Genx Increased The Generation Of Intracellular Reactive Oxygen Species (Ros), Indicating The Induction Of Oxidative Stress In A Dose-Dependent Manner. Genx Treatment Increased The Expression Of Major Apoptosis-Related Genes Relative To The Untreated Control Group.
AB - Hexafluoropropylene Oxide Dimer Acid, Also Known As Genx, Is A Poly-and Perfluoroalkyl Substance (Pfas). Pfass Are Nonvolatile Synthetic Substances That Can Be Readily Disseminated Into The Environment During Processing And Use, Making Them Easy To Implement In The Soil, Drinking Water, And Air. Compared To Other Pfass, Genx Has A Comparatively Short Carbon Chain Length And Is Expected To Have A Lower Tendency To Accumulate In Humans; Therefore, Genx Has Recently Been Used As A Substitute To Other Pfass. However, The Mechanisms Underlying Genx Action And Intoxication In Humans Remains Unclear. In This Study, The Apoptotic Capacity Of Genx In Human Liver Cells Was Investigated. When Representative Human-Derived Liver Cells (Hepg2 Cells) Were Treated With Genx For 12 H, Cell Viability Was Reduced, And Apoptosis Was Greatly Increased. In Addition, Genx Increased The Generation Of Intracellular Reactive Oxygen Species (Ros), Indicating The Induction Of Oxidative Stress In A Dose-Dependent Manner. Genx Treatment Increased The Expression Of Major Apoptosis-Related Genes Relative To The Untreated Control Group.
KW - Apoptosis
KW - Hexafluoropropylene oxide dimer acid
KW - Liver cells
KW - Oxidative stress
KW - Poly and perfluoroalkyl substance
UR - http://www.scopus.com/inward/record.url?scp=85120870815&partnerID=8YFLogxK
U2 - 10.1016/j.heliyon.2021.e08272
DO - 10.1016/j.heliyon.2021.e08272
M3 - Article
AN - SCOPUS:85120870815
SN - 2405-8440
VL - 7
JO - Heliyon
JF - Heliyon
IS - 11
M1 - e08272
ER -