High-density cell culture study of recombinant Escherichia coli was carried out using a membrane cell recycle fermenter. The maximum cell concentration increased tenfold compared to that in conventional batch process. The main inhibitory metabolic product was acetic acid, which accumulated to 13.5 g l-1 and inhibited the cell growth. The stability of recombinant plasmid rapidly declined as the cell concentration increased during the recycle operation.
- dense culture
- recombinant Escherichia coli
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology