High-density culture of Escherichia coli carrying recombinant plasmid in a membrane cell recycle fermenter

Chang Woo Lee; Man Bock Gu; Ho Nam Chang

doi:10.1016/0141-0229(89)90113-0

High-density culture of Escherichia coli carrying recombinant plasmid in a membrane cell recycle fermenter

Chang Woo Lee, Man Bock Gu, Ho Nam Chang

Department of Biotechnology

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

High-density cell culture study of recombinant Escherichia coli was carried out using a membrane cell recycle fermenter. The maximum cell concentration increased tenfold compared to that in conventional batch process. The main inhibitory metabolic product was acetic acid, which accumulated to 13.5 g l^-1 and inhibited the cell growth. The stability of recombinant plasmid rapidly declined as the cell concentration increased during the recycle operation.

Original language	English
Pages (from-to)	49-54
Number of pages	6
Journal	Enzyme and Microbial Technology
Volume	11
Issue number	1
DOIs	https://doi.org/10.1016/0141-0229(89)90113-0
Publication status	Published - 1989 Jan

Keywords

Bioreactor
dense culture
recombinant Escherichia coli
recycle

ASJC Scopus subject areas

Biotechnology
Bioengineering
Biochemistry
Applied Microbiology and Biotechnology

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10.1016/0141-0229(89)90113-0

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abstract = "High-density cell culture study of recombinant Escherichia coli was carried out using a membrane cell recycle fermenter. The maximum cell concentration increased tenfold compared to that in conventional batch process. The main inhibitory metabolic product was acetic acid, which accumulated to 13.5 g l-1 and inhibited the cell growth. The stability of recombinant plasmid rapidly declined as the cell concentration increased during the recycle operation.",

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AU - Lee, Chang Woo

AU - Gu, Man Bock

AU - Chang, Ho Nam

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AB - High-density cell culture study of recombinant Escherichia coli was carried out using a membrane cell recycle fermenter. The maximum cell concentration increased tenfold compared to that in conventional batch process. The main inhibitory metabolic product was acetic acid, which accumulated to 13.5 g l-1 and inhibited the cell growth. The stability of recombinant plasmid rapidly declined as the cell concentration increased during the recycle operation.

KW - Bioreactor

KW - dense culture

KW - recombinant Escherichia coli

KW - recycle

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ER -

High-density culture of Escherichia coli carrying recombinant plasmid in a membrane cell recycle fermenter

Abstract

Keywords

ASJC Scopus subject areas

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