Abstract
High-density cell culture study of recombinant Escherichia coli was carried out using a membrane cell recycle fermenter. The maximum cell concentration increased tenfold compared to that in conventional batch process. The main inhibitory metabolic product was acetic acid, which accumulated to 13.5 g l-1 and inhibited the cell growth. The stability of recombinant plasmid rapidly declined as the cell concentration increased during the recycle operation.
| Original language | English |
|---|---|
| Pages (from-to) | 49-54 |
| Number of pages | 6 |
| Journal | Enzyme and Microbial Technology |
| Volume | 11 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1989 Jan |
Keywords
- Bioreactor
- dense culture
- recombinant Escherichia coli
- recycle
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Biochemistry
- Applied Microbiology and Biotechnology