High-efficiency cell disruption and astaxanthin recovery from Haematococcus pluvialis cyst cells using room-temperature imidazolium-based ionic liquid/water mixtures

Sun A. Choi; You Kwan Oh; Jiye Lee; Sang Jun Sim; Min Eui Hong; Ji Yeon Park; Min Sik Kim; Seung Wook Kim; Jin Suk Lee

doi:10.1016/j.biortech.2018.11.082

High-efficiency cell disruption and astaxanthin recovery from Haematococcus pluvialis cyst cells using room-temperature imidazolium-based ionic liquid/water mixtures

Sun A. Choi, You Kwan Oh, Jiye Lee, Sang Jun Sim, Min Eui Hong, Ji Yeon Park, Min Sik Kim, Seung Wook Kim, Jin Suk Lee

Research output: Contribution to journal › Article › peer-review

81 Citations (Scopus)

Abstract

Energy-saving, high-efficiency cell disruption is a critical step for recovery of thermolabile antioxidant astaxanthin from Haematococcus pluvialis cyst cells of rigid cell-wall structure. In this study, as room-temperature green solvents, 10 types of 1-ethyl-3-methylimidazolium ([Emim])-based ionic liquids (ILs) were compared and evaluated for their abilities to disrupt H. pluvialis cyst cells for astaxanthin/lipid extraction. Among the 10 ILs tested, 3 [Emim]-based ILs with HSO₄, CH₃SO₃, and (CF₃SO₂)₂N anions were selected based on astaxanthin/lipid extraction performance and synthesis cost. When pretreated with IL/water mixtures, intact cyst cells were significantly torn, broken or shown to release cytoplasmic components, thereby facilitating subsequent separation of astaxanthin/lipid by hexane. However, excess IL pretreatments at high temperature/IL dosages and longer incubation times significantly deteriorated lipid and/or astaxanthin. Under optimized mild conditions (6.7% (v/v) IL in water solution, 30 °C, 60 min), almost complete astaxanthin recoveries (>99%) along with moderate lipid extractions (∼82%) could be obtained.

Original language	English
Pages (from-to)	120-126
Number of pages	7
Journal	Bioresource technology
Volume	274
DOIs	https://doi.org/10.1016/j.biortech.2018.11.082
Publication status	Published - 2019 Feb

Bibliographical note

Funding Information:
This work was supported by the Advanced Biomass R&D Center (ABC) of the Global Frontier Project funded by the Ministry of Science and ICT , Republic of Korea ( ABC- 2012M3A6A205388 ). This work was conducted under the framework of the Research and Development Program of the Korea Institute of Energy Research (KIER; B8-2432-04). Prof. S. J. Sim also would like to acknowledge the support of the Korea CCS R&D Center (Korea CCS 2020 Project) funded by the Korea government (Ministry of Science and ICT) in 2017 (grant number: KCRC-2014M1A8A1049278 ).

Funding Information:
This work was supported by the Advanced Biomass R&D Center (ABC) of the Global Frontier Project funded by the Ministry of Science and ICT, Republic of Korea (ABC- 2012M3A6A205388). This work was conducted under the framework of the Research and Development Program of the Korea Institute of Energy Research (KIER; B8-2432-04). Prof. S. J. Sim also would like to acknowledge the support of the Korea CCS R&D Center (Korea CCS 2020 Project) funded by the Korea government (Ministry of Science and ICT) in 2017 (grant number: KCRC-2014M1A8A1049278).

Publisher Copyright:
© 2018

Keywords

Astaxanthin extraction
Cell disruption
Haematococcus pluvialis cyst
Ionic liquid
Lipid extraction

ASJC Scopus subject areas

Bioengineering
Environmental Engineering
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biortech.2018.11.082

Cite this

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title = "High-efficiency cell disruption and astaxanthin recovery from Haematococcus pluvialis cyst cells using room-temperature imidazolium-based ionic liquid/water mixtures",

abstract = "Energy-saving, high-efficiency cell disruption is a critical step for recovery of thermolabile antioxidant astaxanthin from Haematococcus pluvialis cyst cells of rigid cell-wall structure. In this study, as room-temperature green solvents, 10 types of 1-ethyl-3-methylimidazolium ([Emim])-based ionic liquids (ILs) were compared and evaluated for their abilities to disrupt H. pluvialis cyst cells for astaxanthin/lipid extraction. Among the 10 ILs tested, 3 [Emim]-based ILs with HSO4, CH3SO3, and (CF3SO2)2N anions were selected based on astaxanthin/lipid extraction performance and synthesis cost. When pretreated with IL/water mixtures, intact cyst cells were significantly torn, broken or shown to release cytoplasmic components, thereby facilitating subsequent separation of astaxanthin/lipid by hexane. However, excess IL pretreatments at high temperature/IL dosages and longer incubation times significantly deteriorated lipid and/or astaxanthin. Under optimized mild conditions (6.7% (v/v) IL in water solution, 30 °C, 60 min), almost complete astaxanthin recoveries (>99%) along with moderate lipid extractions (∼82%) could be obtained.",

keywords = "Astaxanthin extraction, Cell disruption, Haematococcus pluvialis cyst, Ionic liquid, Lipid extraction",

author = "Choi, {Sun A.} and Oh, {You Kwan} and Jiye Lee and Sim, {Sang Jun} and Hong, {Min Eui} and Park, {Ji Yeon} and Kim, {Min Sik} and Kim, {Seung Wook} and Lee, {Jin Suk}",

note = "Funding Information: This work was supported by the Advanced Biomass R&D Center (ABC) of the Global Frontier Project funded by the Ministry of Science and ICT , Republic of Korea ( ABC- 2012M3A6A205388 ). This work was conducted under the framework of the Research and Development Program of the Korea Institute of Energy Research (KIER; B8-2432-04). Prof. S. J. Sim also would like to acknowledge the support of the Korea CCS R&D Center (Korea CCS 2020 Project) funded by the Korea government (Ministry of Science and ICT) in 2017 (grant number: KCRC-2014M1A8A1049278 ). Funding Information: This work was supported by the Advanced Biomass R&D Center (ABC) of the Global Frontier Project funded by the Ministry of Science and ICT, Republic of Korea (ABC- 2012M3A6A205388). This work was conducted under the framework of the Research and Development Program of the Korea Institute of Energy Research (KIER; B8-2432-04). Prof. S. J. Sim also would like to acknowledge the support of the Korea CCS R&D Center (Korea CCS 2020 Project) funded by the Korea government (Ministry of Science and ICT) in 2017 (grant number: KCRC-2014M1A8A1049278). Publisher Copyright: {\textcopyright} 2018",

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AU - Choi, Sun A.

AU - Oh, You Kwan

AU - Lee, Jiye

AU - Sim, Sang Jun

AU - Hong, Min Eui

AU - Park, Ji Yeon

AU - Kim, Min Sik

AU - Kim, Seung Wook

AU - Lee, Jin Suk

N1 - Funding Information: This work was supported by the Advanced Biomass R&D Center (ABC) of the Global Frontier Project funded by the Ministry of Science and ICT , Republic of Korea ( ABC- 2012M3A6A205388 ). This work was conducted under the framework of the Research and Development Program of the Korea Institute of Energy Research (KIER; B8-2432-04). Prof. S. J. Sim also would like to acknowledge the support of the Korea CCS R&D Center (Korea CCS 2020 Project) funded by the Korea government (Ministry of Science and ICT) in 2017 (grant number: KCRC-2014M1A8A1049278 ). Funding Information: This work was supported by the Advanced Biomass R&D Center (ABC) of the Global Frontier Project funded by the Ministry of Science and ICT, Republic of Korea (ABC- 2012M3A6A205388). This work was conducted under the framework of the Research and Development Program of the Korea Institute of Energy Research (KIER; B8-2432-04). Prof. S. J. Sim also would like to acknowledge the support of the Korea CCS R&D Center (Korea CCS 2020 Project) funded by the Korea government (Ministry of Science and ICT) in 2017 (grant number: KCRC-2014M1A8A1049278). Publisher Copyright: © 2018

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AB - Energy-saving, high-efficiency cell disruption is a critical step for recovery of thermolabile antioxidant astaxanthin from Haematococcus pluvialis cyst cells of rigid cell-wall structure. In this study, as room-temperature green solvents, 10 types of 1-ethyl-3-methylimidazolium ([Emim])-based ionic liquids (ILs) were compared and evaluated for their abilities to disrupt H. pluvialis cyst cells for astaxanthin/lipid extraction. Among the 10 ILs tested, 3 [Emim]-based ILs with HSO4, CH3SO3, and (CF3SO2)2N anions were selected based on astaxanthin/lipid extraction performance and synthesis cost. When pretreated with IL/water mixtures, intact cyst cells were significantly torn, broken or shown to release cytoplasmic components, thereby facilitating subsequent separation of astaxanthin/lipid by hexane. However, excess IL pretreatments at high temperature/IL dosages and longer incubation times significantly deteriorated lipid and/or astaxanthin. Under optimized mild conditions (6.7% (v/v) IL in water solution, 30 °C, 60 min), almost complete astaxanthin recoveries (>99%) along with moderate lipid extractions (∼82%) could be obtained.

KW - Astaxanthin extraction

KW - Cell disruption

KW - Haematococcus pluvialis cyst

KW - Ionic liquid

KW - Lipid extraction

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High-efficiency cell disruption and astaxanthin recovery from Haematococcus pluvialis cyst cells using room-temperature imidazolium-based ionic liquid/water mixtures

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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