High fat diet altered the mechanism of energy homeostasis induced by nicotine and withdrawal in C57BL/6 mice

Young Na Hur, Gee Hyun Hong, Sang Hyun Choi, Kyung Ho Shin, Boe Gwun Chun

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)


Nicotine treatment has known to produce an inverse relationship between body weight and food intake in rodents. Present study determined the effect of repeated treatment with nicotine and withdrawal in control and obese mice, on: (1) body weight, caloric intake and energy expenditure; (2) hypothalamic neuropeptides mRNA expression; and (3) serum leptin. 21-week-old C57BL/6 mice (n = 65) received nicotine (3.0 mg/kg/day; 2 weeks) and saline (1 ml/kg/day; 2 weeks) subcutaneously. Animals were given either a normal-fat (10% kcal from fat, NF) or a high-fat diet (45% kcal from fat, HF) from the 12 th week to 25 th week. While, nicotine treatment for 14 days induced an increase in hypothalamic agouti-related protein, cocaine- and amphetamine- regulated transcript, pro-opiomelanocortin mRNA expressions, nicotine also produced a reducing effect in body weight gain and leptin concentration in NF mice. High-fat diet induced obese mice showed a blunted hypothalamic and leptin response to nicotine. Remarkable weight loss in obese mice was mediated not just by decreasing caloric intake, but also by increasing total energy expenditure (EE). During nicotine withdrawal period, weight gain occurred in NF and HF groups, which was ascribed to a decrease in EE rather than changes in caloric intake. Hypothalamic AgRP might play a role for maintaining energy balance under the nicotine-induced negative energy status.

Original languageEnglish
Pages (from-to)219-226
Number of pages8
JournalMolecules and cells
Issue number3
Publication statusPublished - 2010 Sept
Externally publishedYes

Bibliographical note

Funding Information:
This work was supported in part by the National Research Foundation of Korea grant funded by the Korea government (Ministry of Education, Science and Technology) (M1-0108-00-009) and Korea University.


  • body weight
  • food intake
  • hypothalamic neuropeptides
  • nicotine
  • obesity

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology


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