Abstract
A strain of Alcaligenes eutrophus producing poly-β-hydroxybutyric acid was successfully transformed by the electroporation. The plasmid used was a broad host range plasmid pKT230 conferring kanamycin resistance. The optimum yield of transformant was 0.8×102/μg DNA when 50 μl competent cells at 1010/ml were pulsed by 11.5 kV/cm for 5 ms with 1 μg DNA. Plasmid DNA in the A. eutrophus transformant was stably maintained as a monomeric structure.
Original language | English |
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Pages (from-to) | 31-34 |
Number of pages | 4 |
Journal | Biotechnology Techniques |
Volume | 9 |
Issue number | 1 |
DOIs | |
Publication status | Published - 1995 Jan |
Externally published | Yes |
ASJC Scopus subject areas
- Biochemistry
- Applied Microbiology and Biotechnology