High frequency transformation of Alcaligenes eutrophus producing poly-β-hydroxybutyric acid by electroporation

Hae Chul Park; Kyung Jun Lim; Jin Seo Park; Yong Hyun Lee; Tae Lin Huh

doi:10.1007/BF00152996

High frequency transformation of Alcaligenes eutrophus producing poly-β-hydroxybutyric acid by electroporation

Hae Chul Park, Kyung Jun Lim, Jin Seo Park, Yong Hyun Lee, Tae Lin Huh

Research output: Contribution to journal › Article › peer-review

29 Citations (Scopus)

Abstract

A strain of Alcaligenes eutrophus producing poly-β-hydroxybutyric acid was successfully transformed by the electroporation. The plasmid used was a broad host range plasmid pKT230 conferring kanamycin resistance. The optimum yield of transformant was 0.8×10²/μg DNA when 50 μl competent cells at 10¹⁰/ml were pulsed by 11.5 kV/cm for 5 ms with 1 μg DNA. Plasmid DNA in the A. eutrophus transformant was stably maintained as a monomeric structure.

Original language	English
Pages (from-to)	31-34
Number of pages	4
Journal	Biotechnology Techniques
Volume	9
Issue number	1
DOIs	https://doi.org/10.1007/BF00152996
Publication status	Published - 1995 Jan
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Applied Microbiology and Biotechnology

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10.1007/BF00152996

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title = "High frequency transformation of Alcaligenes eutrophus producing poly-β-hydroxybutyric acid by electroporation",

abstract = "A strain of Alcaligenes eutrophus producing poly-β-hydroxybutyric acid was successfully transformed by the electroporation. The plasmid used was a broad host range plasmid pKT230 conferring kanamycin resistance. The optimum yield of transformant was 0.8×102/μg DNA when 50 μl competent cells at 1010/ml were pulsed by 11.5 kV/cm for 5 ms with 1 μg DNA. Plasmid DNA in the A. eutrophus transformant was stably maintained as a monomeric structure.",

author = "Park, {Hae Chul} and Lim, {Kyung Jun} and Park, {Jin Seo} and Lee, {Yong Hyun} and Huh, {Tae Lin}",

year = "1995",

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AU - Park, Hae Chul

AU - Lim, Kyung Jun

AU - Park, Jin Seo

AU - Lee, Yong Hyun

AU - Huh, Tae Lin

PY - 1995/1

Y1 - 1995/1

N2 - A strain of Alcaligenes eutrophus producing poly-β-hydroxybutyric acid was successfully transformed by the electroporation. The plasmid used was a broad host range plasmid pKT230 conferring kanamycin resistance. The optimum yield of transformant was 0.8×102/μg DNA when 50 μl competent cells at 1010/ml were pulsed by 11.5 kV/cm for 5 ms with 1 μg DNA. Plasmid DNA in the A. eutrophus transformant was stably maintained as a monomeric structure.

AB - A strain of Alcaligenes eutrophus producing poly-β-hydroxybutyric acid was successfully transformed by the electroporation. The plasmid used was a broad host range plasmid pKT230 conferring kanamycin resistance. The optimum yield of transformant was 0.8×102/μg DNA when 50 μl competent cells at 1010/ml were pulsed by 11.5 kV/cm for 5 ms with 1 μg DNA. Plasmid DNA in the A. eutrophus transformant was stably maintained as a monomeric structure.

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DO - 10.1007/BF00152996

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JO - Biotechnology Techniques

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IS - 1

ER -

High frequency transformation of Alcaligenes eutrophus producing poly-β-hydroxybutyric acid by electroporation

Abstract

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