Abstract
Ion concentration polarization (ICP) preconcentration is a pretreatment method that is actively utilized in the analysis of low-abundance biomolecules. In biofluids (e.g. urine, sweat, blood, serum, plasma, saliva, etc.), low-concentrated target analytes presented among large number of background molecules under a high ionic concentration, there still exist several hurdles in the utilization of ICP preconcentration in the such high ionic fluids. This is especially true in the case of blood-based biofluids, such as plasma and serum, wherein on account of the abundance biomolecules as well as high ionic buffer concentration makes the ICP preconcentration process difficult to realize. In this study, we have demonstrated the realization of ICP preconcentration of blood-based biofluids (serum) using a paper-based preconcentrator. We acquired preconcentration factors (PF) for 25.5, 127.5 and 255 mM of human serum were 40, 10, and 5.5 within 20 min. The squared fluorescence intensity is also found to be inversely proportional to ionic concentration, showing that the PF are strongly correlated to the Debye length of the buffer solution. Using the paper-based preconcentrator, we were able to acquire effective preconcentration factor (PFeff) of 5.17, 4.88, 4.58, and 4.23 for the 1X, 0.75X, 0.5X, and 0.1X diluted solutions, demonstrating approximately 5 times increase in PFeff for all prepared human serum samples.
| Original language | English |
|---|---|
| Pages (from-to) | 485-493 |
| Number of pages | 9 |
| Journal | Sensors and Actuators, B: Chemical |
| Volume | 268 |
| DOIs | |
| Publication status | Published - 2018 Sept 1 |
Bibliographical note
Funding Information:The authors are very grateful for the financial support received from the Korea Health Industry Development Institute (KHIDI, HI14C3319 ) and the National Research Foundation of Korea , Grant ( NRF-2015R1D1A1A01059806 ). J. H. Lee was also supported by a research grant from Kwangwoon University in 2018 . Sung Il Han received the B.S., and M.S. degrees from the Department of Electrical Engineering of Kwangwoon University, Seoul, Korea. He is a Ph. D. candidate in the Department of Electrical Engineering of Kwangwoon University, Seoul, Korea. His current research is focused on microfluidic paper-based analytical devices using ion concentration polarization. Yong Kyoung Yoo receviced the B.S., M.S., and Ph.D. degrees from Kwangwoon University, Seoul, Korea in 2011, 2013 and 2017. He joined the Department of Clinical Pharmacology and Therapeutics, College of Medicine, Kyung Hee University, Seoul, Republic of Korea in 2017, where he is as an post doctoral course. His current research is focused on biosensor and bioelectronics. Jeong Hoon Lee is a professor at Electrical Engineering, Kwangwoon University, South Korea. He received the B.S. degree in the department of Ceramic Engineering at Yonsei University, Seoul, South Korea, in 1997. He received the Ph.D from same department in 2004. He specialized in MEMS/Nanomechanics from 1999 to 2005 at Korea Institute of Science and Technology (KIST) in Seoul, South Korea. Before joining Kwangwoon University in Sep 2008, he was a Postdoctoral Associate at RLE and EECS, Massachusetts Institute of Technology (MIT), USA (2005–2008). His current main research is the development of simple and powerful POCT and diagnostic systems based on the integration of electronics and fluidics.
Publisher Copyright:
© 2018 Elsevier B.V.
Keywords
- Biofluids
- Human serum
- Ion concentration polarization (ICP)
- Paper-chip
- Sample preparation
- μPADs
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Instrumentation
- Condensed Matter Physics
- Surfaces, Coatings and Films
- Metals and Alloys
- Electrical and Electronic Engineering
- Materials Chemistry