Decellularization of tissues provides extracellular matrix (ECM) scaffolds for regeneration therapy and an experimental model to understand ECM and cellular interactions. However, decellularization often causes microstructure disintegration and reduction of physical strength, which greatly limits the use of this technique in soft organs or in applications that require maintenance of physical strength. Here, we present a new tissue decellularization procedure, namely CASPER (Clinically and Experimentally Applicable Acellular Tissue Scaffold Production for Tissue Engineering and Regenerative Medicine), which includes infusion and hydrogel polymerization steps prior to robust chemical decellularization treatments. Polymerized hydrogels serve to prevent excessive damage to the ECM while maintaining the sophisticated structures and biological activities of ECM components in various organs, including soft tissues such as brains and embryos. CASPERized tissues were successfully recellularized to stimulate a tissue-regeneration-like process after implantation without signs of pathological inflammation or fibrosis in vivo, suggesting that CASPERized tissues can be used for monitoring cell-ECM interactions and for surrogate organ transplantation.
Bibliographical noteFunding Information:
We greatly appreciate the technical support of Ms. Jieun Na for SEM analysis. We also thank the Korea Institute of Science and Technology (KIST) for helping to measure physical strength. Instruments (IMARIS) was supported by Brain Research Core Facilities in Korea Brain Research Institute (KBRI). This research was supported by the Brain Research Program through the National Research Foundation (NRF) funded by the Korean Ministry of Science, ICT & Future Planning (NRF-2015M3C7A1028790, 2018R1D1A1A02086190), KBRI basic research program through the Korea Brain Research Institute funded by the Ministry of Science and ICT (19-BR-02-01).
© 2019 American Chemical Society.
- hernia repair
- tissue decellularization
- tissue engineering
- tissue regeneration
- tissue scaffold
ASJC Scopus subject areas
- Biomedical Engineering