TY - JOUR
T1 - High-sensitivity detection of silver ions using oligonucleotide-immobilized oscillator
AU - Park, Jinsung
AU - Choi, Wook
AU - Jang, Kuewhan
AU - Na, Sungsoo
N1 - Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) under Grant nos. NRF-2012-0000785 and NRF-2011-0020090 .
PY - 2013/3/15
Y1 - 2013/3/15
N2 - With the remarkable developments in the fields of nanoscale research and industry, nanotoxicity is gaining importance from the viewpoint of its potential impact on human health and the environment. Herein, we report on the label-free, high-sensitivity detection of Ag+, a representative nanotoxic material, by using a silver-specific nucleotide-coated oscillator. The detection is based on the measurement of the resonant frequency shift arising from constitution of the cytosine-Ag+-cytosine bonding. We amplify the resonant frequency shift by using single cytosine molecules. It is shown that a silver-specific DNA-immobilized oscillator enables the capture of silver ions at concentrations below 1nM. Remarkably, the nucleotide-based oscillator enables an insight into the coordination chemistry, which plays an important role in the early detection of toxicity. This implies that the bio-conjugated sensor could be used to set the reference point for water quality.
AB - With the remarkable developments in the fields of nanoscale research and industry, nanotoxicity is gaining importance from the viewpoint of its potential impact on human health and the environment. Herein, we report on the label-free, high-sensitivity detection of Ag+, a representative nanotoxic material, by using a silver-specific nucleotide-coated oscillator. The detection is based on the measurement of the resonant frequency shift arising from constitution of the cytosine-Ag+-cytosine bonding. We amplify the resonant frequency shift by using single cytosine molecules. It is shown that a silver-specific DNA-immobilized oscillator enables the capture of silver ions at concentrations below 1nM. Remarkably, the nucleotide-based oscillator enables an insight into the coordination chemistry, which plays an important role in the early detection of toxicity. This implies that the bio-conjugated sensor could be used to set the reference point for water quality.
KW - DNA-metal interaction
KW - Micro-oscillator
KW - Resonant frequency
KW - Scanning probe microscopy
KW - Silver ion
UR - http://www.scopus.com/inward/record.url?scp=84870773194&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2012.09.014
DO - 10.1016/j.bios.2012.09.014
M3 - Article
C2 - 23058660
AN - SCOPUS:84870773194
SN - 0956-5663
VL - 41
SP - 471
EP - 476
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
IS - 1
ER -