High-speed lateral flow strategy for a fast biosensing with an improved selectivity and binding affinity

Dong Guk Cho; Haneul Yoo; Haein Lee; Yeol Kyo Choi; Minju Lee; Dong June Ahn; Seunghun Hong

doi:10.3390/s18051507

High-speed lateral flow strategy for a fast biosensing with an improved selectivity and binding affinity

Dong Guk Cho, Haneul Yoo, Haein Lee, Yeol Kyo Choi, Minju Lee, Dong June Ahn, Seunghun Hong

Department of Chemical and Biological Engineering

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

We report a high-speed lateral flow strategy for a fast biosensing with an improved selectivity and binding affinity even under harsh conditions. In this strategy, biosensors were fixed at a location away from the center of a round shape disk, and the disk was rotated to create the lateral flow of a target solution on the biosensors during the sensing measurements. Experimental results using the strategy showed high reaction speeds, high binding affinity, and low nonspecific adsorptions of target molecules to biosensors. Furthermore, binding affinity between target molecules and sensing molecules was enhanced even in harsh conditions such as low pH and low ionic strength conditions. These results show that the strategy can improve the performance of conventional biosensors by generating high-speed lateral flows on a biosensor surface. Therefore, our strategy can be utilized as a simple but powerful tool for versatile bio and medical applications.

Original language	English
Article number	1507
Journal	Sensors (Switzerland)
Volume	18
Issue number	5
DOIs	https://doi.org/10.3390/s18051507
Publication status	Published - 2018 May 10

Bibliographical note

Funding Information:
Funding: This research was funded by National Research Foundation (NRF), grant numbers 2013M3A6B2078961, 2014M3A7B4051591, 2017R1A2B2006808, and 2017R1A2B3006770. This project has also received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement No 682286).

Funding Information:
Acknowledgments: H.L., Y.K.C. and D.J.A. acknowledge the support from the Korea University Grant.

Funding Information:
This research was funded by National Research Foundation (NRF), grant numbers 2013M3A6B2078961, 2014M3A7B4051591, 2017R1A2B2006808, and 2017R1A2B3006770. This project has also received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement No 682286).

Publisher Copyright:
© 2018 by the authors; licensee MDPI, Basel, Switzerland.

Keywords

Binding affinity
Lateral flow
Reaction speed
Rotating disk
Selectivity

ASJC Scopus subject areas

Analytical Chemistry
Information Systems
Atomic and Molecular Physics, and Optics
Biochemistry
Instrumentation
Electrical and Electronic Engineering

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10.3390/s18051507

Cite this

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abstract = "We report a high-speed lateral flow strategy for a fast biosensing with an improved selectivity and binding affinity even under harsh conditions. In this strategy, biosensors were fixed at a location away from the center of a round shape disk, and the disk was rotated to create the lateral flow of a target solution on the biosensors during the sensing measurements. Experimental results using the strategy showed high reaction speeds, high binding affinity, and low nonspecific adsorptions of target molecules to biosensors. Furthermore, binding affinity between target molecules and sensing molecules was enhanced even in harsh conditions such as low pH and low ionic strength conditions. These results show that the strategy can improve the performance of conventional biosensors by generating high-speed lateral flows on a biosensor surface. Therefore, our strategy can be utilized as a simple but powerful tool for versatile bio and medical applications.",

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N2 - We report a high-speed lateral flow strategy for a fast biosensing with an improved selectivity and binding affinity even under harsh conditions. In this strategy, biosensors were fixed at a location away from the center of a round shape disk, and the disk was rotated to create the lateral flow of a target solution on the biosensors during the sensing measurements. Experimental results using the strategy showed high reaction speeds, high binding affinity, and low nonspecific adsorptions of target molecules to biosensors. Furthermore, binding affinity between target molecules and sensing molecules was enhanced even in harsh conditions such as low pH and low ionic strength conditions. These results show that the strategy can improve the performance of conventional biosensors by generating high-speed lateral flows on a biosensor surface. Therefore, our strategy can be utilized as a simple but powerful tool for versatile bio and medical applications.

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High-speed lateral flow strategy for a fast biosensing with an improved selectivity and binding affinity

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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