High speed slit-scanning confocal laser microscopy with an acousto-optic beam deflector and a line scan camera

Sumin Han; Kang Bin Im; Hwajoon Park; Dongsun Kim; Beop Min Kim

doi:10.1117/12.591465

High speed slit-scanning confocal laser microscopy with an acousto-optic beam deflector and a line scan camera

Sumin Han, Kang Bin Im, Hwajoon Park, Dongsun Kim, Beop Min Kim

Research output: Contribution to journal › Conference article › peer-review

2 Citations (Scopus)

Abstract

Using an acousto-optic beam deflector and a line scan camera, we constructed a non-mechanical, slit-scanning confocal microscope. It generates two-dimensional 512×512 images with speed of 60 frames/sec, which can be easily expanded, to 100 frames/sec. The measured axial and lateral resolutions of the system are 3.3 μm and <1 μm, respectively, with a 50X objective. This simple design can produce frames rates faster than other commercial products with comparable resolution, which may be useful for analysis of rapid interactions in various biological applications.

Original language	English
Article number	33
Pages (from-to)	164-171
Number of pages	8
Journal	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	5701
DOIs	https://doi.org/10.1117/12.591465
Publication status	Published - 2005
Externally published	Yes
Event	Three- Dimensional and Multidomensional Microscopy: Image Acquisition and Processing XII - San Jose, CA, United States Duration: 2005 Jan 25 → 2005 Jan 27

Keywords

Acousto-optic deflector
Confocal microscope
Line scan CCD
Three-dimensional microscopy

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Biomaterials
Atomic and Molecular Physics, and Optics
Radiology Nuclear Medicine and imaging

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10.1117/12.591465

Cite this

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title = "High speed slit-scanning confocal laser microscopy with an acousto-optic beam deflector and a line scan camera",

abstract = "Using an acousto-optic beam deflector and a line scan camera, we constructed a non-mechanical, slit-scanning confocal microscope. It generates two-dimensional 512×512 images with speed of 60 frames/sec, which can be easily expanded, to 100 frames/sec. The measured axial and lateral resolutions of the system are 3.3 μm and <1 μm, respectively, with a 50X objective. This simple design can produce frames rates faster than other commercial products with comparable resolution, which may be useful for analysis of rapid interactions in various biological applications.",

keywords = "Acousto-optic deflector, Confocal microscope, Line scan CCD, Three-dimensional microscopy",

author = "Sumin Han and Im, {Kang Bin} and Hwajoon Park and Dongsun Kim and Kim, {Beop Min}",

year = "2005",

doi = "10.1117/12.591465",

language = "English",

volume = "5701",

pages = "164--171",

journal = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

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note = "Three- Dimensional and Multidomensional Microscopy: Image Acquisition and Processing XII ; Conference date: 25-01-2005 Through 27-01-2005",

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TY - JOUR

T1 - High speed slit-scanning confocal laser microscopy with an acousto-optic beam deflector and a line scan camera

AU - Han, Sumin

AU - Im, Kang Bin

AU - Park, Hwajoon

AU - Kim, Dongsun

AU - Kim, Beop Min

PY - 2005

Y1 - 2005

N2 - Using an acousto-optic beam deflector and a line scan camera, we constructed a non-mechanical, slit-scanning confocal microscope. It generates two-dimensional 512×512 images with speed of 60 frames/sec, which can be easily expanded, to 100 frames/sec. The measured axial and lateral resolutions of the system are 3.3 μm and <1 μm, respectively, with a 50X objective. This simple design can produce frames rates faster than other commercial products with comparable resolution, which may be useful for analysis of rapid interactions in various biological applications.

AB - Using an acousto-optic beam deflector and a line scan camera, we constructed a non-mechanical, slit-scanning confocal microscope. It generates two-dimensional 512×512 images with speed of 60 frames/sec, which can be easily expanded, to 100 frames/sec. The measured axial and lateral resolutions of the system are 3.3 μm and <1 μm, respectively, with a 50X objective. This simple design can produce frames rates faster than other commercial products with comparable resolution, which may be useful for analysis of rapid interactions in various biological applications.

KW - Acousto-optic deflector

KW - Confocal microscope

KW - Line scan CCD

KW - Three-dimensional microscopy

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U2 - 10.1117/12.591465

DO - 10.1117/12.591465

M3 - Conference article

AN - SCOPUS:21844455831

SN - 1605-7422

VL - 5701

SP - 164

EP - 171

JO - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

JF - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

M1 - 33

T2 - Three- Dimensional and Multidomensional Microscopy: Image Acquisition and Processing XII

Y2 - 25 January 2005 through 27 January 2005

ER -

High speed slit-scanning confocal laser microscopy with an acousto-optic beam deflector and a line scan camera

Abstract

Keywords

ASJC Scopus subject areas

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