TY - JOUR
T1 - High-Throughput Separation of Microvesicles from Whole Blood Components Using Viscoelastic Fluid
AU - Nam, Jeonghun
AU - Yoon, Jung
AU - Jee, Hyunseul
AU - Jang, Woong Sik
AU - Lim, Chae Seung
N1 - Funding Information:
J.N. and J.Y. contributed equally to this work. This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No. 2020R1A2C1014460). This research was supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HR20C0021).
Funding Information:
J.N. and J.Y. contributed equally to this work. This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No. 2020R1A2C1014460). This research was supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HR20C0021).
Publisher Copyright:
© 2020 Wiley-VCH GmbH
PY - 2020/12
Y1 - 2020/12
N2 - Cell-derived microvesicles (MVs, also known as microparticles, MPs) are known as important biomarkers of various diseases including cardiovascular diseases, infectious diseases, and cancer. Development of techniques for blood cell removal and MV isolation is required for downstream analyses in biological and clinical applications. A sheathless, label-free, viscoelastic microfluidic device is proposed for the separation of submicrometer platelet-derived MPs (PDMPs) from whole blood. Flow rate and channel length dependent performance of the device is evaluated using 2-µm particles as analogs to platelets and hematocrit (Hct) dependent flow characteristics of red blood cells (RBCs) are observed. In addition, the removal rate of nanoparticles of sizes 300, 500, 700, and 1000 nm is evaluated, and finally, the device is applied to examine the recovery of PDMPs from diluted whole blood sample. At Q = 200 µL min−1 in a 15-mm long microchannel, RBCs at 5% Hct and platelets are removed to the central outlet without platelet activation, while PDMPs are successfully isolated to the side outlets within ≈2 min. The PDMPs collected from the device are analyzed by flow cytometry. The device exhibits ≈4.8-fold enhanced isolation performance compared to the centrifugation method.
AB - Cell-derived microvesicles (MVs, also known as microparticles, MPs) are known as important biomarkers of various diseases including cardiovascular diseases, infectious diseases, and cancer. Development of techniques for blood cell removal and MV isolation is required for downstream analyses in biological and clinical applications. A sheathless, label-free, viscoelastic microfluidic device is proposed for the separation of submicrometer platelet-derived MPs (PDMPs) from whole blood. Flow rate and channel length dependent performance of the device is evaluated using 2-µm particles as analogs to platelets and hematocrit (Hct) dependent flow characteristics of red blood cells (RBCs) are observed. In addition, the removal rate of nanoparticles of sizes 300, 500, 700, and 1000 nm is evaluated, and finally, the device is applied to examine the recovery of PDMPs from diluted whole blood sample. At Q = 200 µL min−1 in a 15-mm long microchannel, RBCs at 5% Hct and platelets are removed to the central outlet without platelet activation, while PDMPs are successfully isolated to the side outlets within ≈2 min. The PDMPs collected from the device are analyzed by flow cytometry. The device exhibits ≈4.8-fold enhanced isolation performance compared to the centrifugation method.
KW - isolation
KW - platelet-derived microparticles
KW - quantification
KW - submicrometer particles
KW - viscoelastic fluids
UR - http://www.scopus.com/inward/record.url?scp=85096696594&partnerID=8YFLogxK
U2 - 10.1002/admt.202000612
DO - 10.1002/admt.202000612
M3 - Article
AN - SCOPUS:85096696594
SN - 2365-709X
VL - 5
JO - Advanced Materials Technologies
JF - Advanced Materials Technologies
IS - 12
M1 - 2000612
ER -
