High-throughput SNP markers for authentication of Korean wheat cultivars based on seven complete plastomes and the nuclear genome

Woohyeon Cho, Jin Kee Jung, Min Young Kang, Yong Weon Seo, Jee Young Park, Tae Jin Yang

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Wheat (Triticum aestivum) has diverse uses in the food industry, and different cultivars have unique properties; therefore, it is important to select the optimal cultivar for the intended end use. Here, to establish an identification system for Korean wheat cultivars, we obtained the complete plastome sequences of seven major Korean cultivars. Additionally, the open access database CerealsDB was queried to discover single-copy genomic single-nucleotide polymorphisms (SNPs) in the hexaploid wheat genome. Ten SNPs were developed into allele-specific PCR (ASP) markers, and eight of the SNPs used for ASP markers were converted into TaqMan high-throughput genotyping markers. Phylogenetic analysis using SNP genotypes revealed the genetic diversity and relationships among 137 wheat lines from around the world, including 35 Korean cultivars. This research thus presents a high-throughput authentication system for Korean wheat cultivars that may promote food industry uses of Korean wheat.

Original languageEnglish
Pages (from-to)423-431
Number of pages9
JournalFood Science and Biotechnology
Volume31
Issue number4
DOIs
Publication statusPublished - 2022 Apr

Bibliographical note

Funding Information:
This work was supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET) through Innovative Food Product and Natural Food Materials Development Program funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA) (320010-02-1-HD030).

Publisher Copyright:
© 2022, The Korean Society of Food Science and Technology.

Keywords

  • Authentication
  • Fluorescence
  • Plastome
  • Polyploidy
  • Wheat

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology

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