High-yield biohydrogen production from biodiesel manufacturing waste by Thermotoga neapolitana

Tien Anh Ngo; Mi Sun Kim; Sang Jun Sim

doi:10.1016/j.ijhydene.2010.11.057

High-yield biohydrogen production from biodiesel manufacturing waste by Thermotoga neapolitana

Tien Anh Ngo, Mi Sun Kim, Sang Jun Sim

Department of Chemical and Biological Engineering

Research output: Contribution to journal › Article › peer-review

91 Citations (Scopus)

Abstract

Efficient conversion of glycerol waste from biodiesel manufacturing processes into biohydrogen by the hyperthermophilic eubacterium Thermotoga neapolitana DSM 4359 was investigated. Biohydrogen production by T. neapolitana was examined using the batch cultivation mode in culture medium containing pure glycerol or glycerol waste as the sole substrate. Pre-treated glycerol waste showed higher hydrogen (H₂) production than untreated waste. Nitrogen (N₂) sparging and pH control were successfully implemented to maintain the culture pH and to reduce H₂ partial pressure in the headspace for optimal growth rate and to enhance hydrogen production from the glycerol waste. It was found that hydrogen production increased from 1.24 ± 0.06 to 1.98 ± 0.1 mol-H₂ mol^-1 glycerol_consumed by optimising N₂ sparging and pH control. We observed that in medium containing 0.05 M HEPES, with three cycles of N ₂ sparging, the H₂ yield increased to 2.73 ± 0.14 mol-H₂ mol^-1 glycerol_consumed, which was 2.22-fold higher than the non-N₂ sparged H₂ yield (1.23 ± 0.06 mol-H₂ mol^-1 glycerol_consumed).

Original language	English
Pages (from-to)	5836-5842
Number of pages	7
Journal	International Journal of Hydrogen Energy
Volume	36
Issue number	10
DOIs	https://doi.org/10.1016/j.ijhydene.2010.11.057
Publication status	Published - 2011 May

Bibliographical note

Funding Information:
This work was supported by the Hydrogen Energy R&D Centre (a 21st Century Frontier R&D Programs) and the Carbon Dioxide Reduction and Sequestration Research Centre (a 21st Century Frontier Program), which are funded by the Ministry of Education Science and Technology of Korea .

Keywords

Biodiesel
Biohydrogen
Buffer
Glycerol waste
Pre-treatment
Thermotoga neapolitana

ASJC Scopus subject areas

Renewable Energy, Sustainability and the Environment
Fuel Technology
Condensed Matter Physics
Energy Engineering and Power Technology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.ijhydene.2010.11.057

Cite this

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title = "High-yield biohydrogen production from biodiesel manufacturing waste by Thermotoga neapolitana",

abstract = "Efficient conversion of glycerol waste from biodiesel manufacturing processes into biohydrogen by the hyperthermophilic eubacterium Thermotoga neapolitana DSM 4359 was investigated. Biohydrogen production by T. neapolitana was examined using the batch cultivation mode in culture medium containing pure glycerol or glycerol waste as the sole substrate. Pre-treated glycerol waste showed higher hydrogen (H2) production than untreated waste. Nitrogen (N2) sparging and pH control were successfully implemented to maintain the culture pH and to reduce H2 partial pressure in the headspace for optimal growth rate and to enhance hydrogen production from the glycerol waste. It was found that hydrogen production increased from 1.24 ± 0.06 to 1.98 ± 0.1 mol-H2 mol-1 glycerolconsumed by optimising N2 sparging and pH control. We observed that in medium containing 0.05 M HEPES, with three cycles of N 2 sparging, the H2 yield increased to 2.73 ± 0.14 mol-H2 mol-1 glycerolconsumed, which was 2.22-fold higher than the non-N2 sparged H2 yield (1.23 ± 0.06 mol-H2 mol-1 glycerolconsumed).",

keywords = "Biodiesel, Biohydrogen, Buffer, Glycerol waste, Pre-treatment, Thermotoga neapolitana",

author = "Ngo, {Tien Anh} and Kim, {Mi Sun} and Sim, {Sang Jun}",

note = "Funding Information: This work was supported by the Hydrogen Energy R&D Centre (a 21st Century Frontier R&D Programs) and the Carbon Dioxide Reduction and Sequestration Research Centre (a 21st Century Frontier Program), which are funded by the Ministry of Education Science and Technology of Korea .",

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doi = "10.1016/j.ijhydene.2010.11.057",

language = "English",

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AU - Ngo, Tien Anh

AU - Kim, Mi Sun

AU - Sim, Sang Jun

N1 - Funding Information: This work was supported by the Hydrogen Energy R&D Centre (a 21st Century Frontier R&D Programs) and the Carbon Dioxide Reduction and Sequestration Research Centre (a 21st Century Frontier Program), which are funded by the Ministry of Education Science and Technology of Korea .

PY - 2011/5

Y1 - 2011/5

N2 - Efficient conversion of glycerol waste from biodiesel manufacturing processes into biohydrogen by the hyperthermophilic eubacterium Thermotoga neapolitana DSM 4359 was investigated. Biohydrogen production by T. neapolitana was examined using the batch cultivation mode in culture medium containing pure glycerol or glycerol waste as the sole substrate. Pre-treated glycerol waste showed higher hydrogen (H2) production than untreated waste. Nitrogen (N2) sparging and pH control were successfully implemented to maintain the culture pH and to reduce H2 partial pressure in the headspace for optimal growth rate and to enhance hydrogen production from the glycerol waste. It was found that hydrogen production increased from 1.24 ± 0.06 to 1.98 ± 0.1 mol-H2 mol-1 glycerolconsumed by optimising N2 sparging and pH control. We observed that in medium containing 0.05 M HEPES, with three cycles of N 2 sparging, the H2 yield increased to 2.73 ± 0.14 mol-H2 mol-1 glycerolconsumed, which was 2.22-fold higher than the non-N2 sparged H2 yield (1.23 ± 0.06 mol-H2 mol-1 glycerolconsumed).

AB - Efficient conversion of glycerol waste from biodiesel manufacturing processes into biohydrogen by the hyperthermophilic eubacterium Thermotoga neapolitana DSM 4359 was investigated. Biohydrogen production by T. neapolitana was examined using the batch cultivation mode in culture medium containing pure glycerol or glycerol waste as the sole substrate. Pre-treated glycerol waste showed higher hydrogen (H2) production than untreated waste. Nitrogen (N2) sparging and pH control were successfully implemented to maintain the culture pH and to reduce H2 partial pressure in the headspace for optimal growth rate and to enhance hydrogen production from the glycerol waste. It was found that hydrogen production increased from 1.24 ± 0.06 to 1.98 ± 0.1 mol-H2 mol-1 glycerolconsumed by optimising N2 sparging and pH control. We observed that in medium containing 0.05 M HEPES, with three cycles of N 2 sparging, the H2 yield increased to 2.73 ± 0.14 mol-H2 mol-1 glycerolconsumed, which was 2.22-fold higher than the non-N2 sparged H2 yield (1.23 ± 0.06 mol-H2 mol-1 glycerolconsumed).

KW - Biodiesel

KW - Biohydrogen

KW - Buffer

KW - Glycerol waste

KW - Pre-treatment

KW - Thermotoga neapolitana

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High-yield biohydrogen production from biodiesel manufacturing waste by Thermotoga neapolitana

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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