High-yield biohydrogen production from biodiesel manufacturing waste by Thermotoga neapolitana

Tien Anh Ngo, Mi Sun Kim, Sang Jun Sim

Research output: Contribution to journalArticlepeer-review

91 Citations (Scopus)


Efficient conversion of glycerol waste from biodiesel manufacturing processes into biohydrogen by the hyperthermophilic eubacterium Thermotoga neapolitana DSM 4359 was investigated. Biohydrogen production by T. neapolitana was examined using the batch cultivation mode in culture medium containing pure glycerol or glycerol waste as the sole substrate. Pre-treated glycerol waste showed higher hydrogen (H2) production than untreated waste. Nitrogen (N2) sparging and pH control were successfully implemented to maintain the culture pH and to reduce H2 partial pressure in the headspace for optimal growth rate and to enhance hydrogen production from the glycerol waste. It was found that hydrogen production increased from 1.24 ± 0.06 to 1.98 ± 0.1 mol-H2 mol-1 glycerolconsumed by optimising N2 sparging and pH control. We observed that in medium containing 0.05 M HEPES, with three cycles of N 2 sparging, the H2 yield increased to 2.73 ± 0.14 mol-H2 mol-1 glycerolconsumed, which was 2.22-fold higher than the non-N2 sparged H2 yield (1.23 ± 0.06 mol-H2 mol-1 glycerolconsumed).

Original languageEnglish
Pages (from-to)5836-5842
Number of pages7
JournalInternational Journal of Hydrogen Energy
Issue number10
Publication statusPublished - 2011 May

Bibliographical note

Funding Information:
This work was supported by the Hydrogen Energy R&D Centre (a 21st Century Frontier R&D Programs) and the Carbon Dioxide Reduction and Sequestration Research Centre (a 21st Century Frontier Program), which are funded by the Ministry of Education Science and Technology of Korea .


  • Biodiesel
  • Biohydrogen
  • Buffer
  • Glycerol waste
  • Pre-treatment
  • Thermotoga neapolitana

ASJC Scopus subject areas

  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology
  • Condensed Matter Physics
  • Energy Engineering and Power Technology


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