Abstract
Lignocellulosic biomass shows high potential as a renewable feedstock for use in biodiesel production via microbial fermentation. Yarrowia lipolytica, an emerging oleaginous yeast, has been engineered to efficiently convert xylose, the second most abundant sugar in lignocellulosic biomass, into lipids for lignocellulosic biodiesel production. Yet, the lipid yield from xylose or lignocellulosic biomass remains far lower than that from glucose. Here we developed an efficient xylose-utilizing Y. lipolytica strain, expressing an isomerase-based pathway, to achieve high-yield lipid production from lignocellulosic biomass. The newly developed xylose-utilizing Y. lipolytica, YSXID, produced 12.01 g/L lipids with a maximum yield of 0.16 g/g, the highest ever reported, from lignocellulosic hydrolysates. Consequently, this study shows the potential of isomerase-based xylose-utilizing Y. lipolytica for economical and sustainable production of biodiesel and oleochemicals from lignocellulosic biomass.
Original language | English |
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Pages (from-to) | 670-679 |
Number of pages | 10 |
Journal | GCB Bioenergy |
Volume | 12 |
Issue number | 9 |
DOIs | |
Publication status | Published - 2020 Sept 1 |
Bibliographical note
Publisher Copyright:© 2020 The Authors. GCB Bioenergy Published by John Wiley & Sons Ltd
Keywords
- Yarrowia lipolytica
- biodiesel
- isomerase-based pathway
- lignocellulosic biofuel
- lignocellulosic hydrolysate
- lipid production
- xylose utilization
ASJC Scopus subject areas
- Forestry
- Renewable Energy, Sustainability and the Environment
- Agronomy and Crop Science
- Waste Management and Disposal