Highly efficient enzyme immobilization and stabilization within meso-structured onion-like silica for biodiesel production

Seung Hyun Jun, Jinwoo Lee, Byoung Chan Kim, Ji Eun Lee, Jin Joo, Hyunmin Park, Jong Ho Lee, Sang Mok Lee, Dohoon Lee, Sangyong Kim, Yoon Mo Koo, Chae Ho Shin, Seung Wook Kim, Taeghwan Hyeon, Jungbae Kim

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69 Citations (Scopus)


Meso-structured onion-like silica (Meso-Onion-S) was synthesized and used as a host of enzyme immobilization. Meso-Onion-S has a 200-300 nm sized primary meso-structured onion building unit, and each onion unit has highly curved mesopores of 10 nm diameter in a multishell structure. Nanoscale enzyme reactors (NERs) in Meso-Onion-S were prepared via a two-step process of enzyme adsorption and subsequent enzyme cross-linking, which effectively prevents the leaching of cross-linked enzyme aggregates from highly curved mesopores of Meso-Onion-S. As a result, NERs in Meso-Onion-S significantly improved the enzyme stability as well as the enzyme loading. For example, NER of lipase (NER-LP) was stable under rigorous shaking for 40 days, while the control sample of adsorbed LP (ADS-LP) with no enzyme cross-linking showed a rapid inactivation due to rigorous enzyme leaching under shaking. Stable NER-LP was successfully employed to produce biodiesels and fatty acid methyl esters, from the LP-catalyzed transesterification of soybean oil with methanol. Interestingly, the specific activity of NER-LP was 23 and 10 times higher than those of free LP and ADS-LP, respectively, revealing the importance of LP stabilization in the form of NER-LP in the presence of organic solvents.

Original languageEnglish
Pages (from-to)924-929
Number of pages6
JournalChemistry of Materials
Issue number5
Publication statusPublished - 2012 Mar 13


  • biodiesel production
  • enzyme immobilization
  • enzyme stabilization
  • meso-structured onion-like silica

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)
  • Materials Chemistry


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