Abstract
Base editors (BEs) composed of a cytidine deaminase fused to CRISPR-Cas9 convert cytidine to uridine, leading to single-base-pair substitutions in eukaryotic cells. We delivered BE mRNA or ribonucleoproteins targeting the Dmd or Tyr gene via electroporation or microinjection into mouse zygotes. F0 mice showed nonsense mutations with an efficiency of 44-57% and allelic frequencies of up to 100%, demonstrating an efficient method to generate mice with targeted point mutations.
Original language | English |
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Pages (from-to) | 435-437 |
Number of pages | 3 |
Journal | Nature Biotechnology |
Volume | 35 |
Issue number | 5 |
DOIs | |
Publication status | Published - 2017 May 1 |
Externally published | Yes |
Bibliographical note
Publisher Copyright:© 2017 Nature America, Inc., part of Springer Nature. All rights reserved.
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology
- Molecular Medicine
- Biomedical Engineering