Highly L and D enantioselective variants of horseradish peroxidase discovered by an ultrahigh-throughput selection method

Eugene Antipov; Art E. Cho; K. Dane Wittrup; Alexander M. Klibanova

doi:10.1073/pnas.0809851105

Highly L and D enantioselective variants of horseradish peroxidase discovered by an ultrahigh-throughput selection method

Eugene Antipov
, Art E. Cho
, K. Dane Wittrup
, Alexander M. Klibanova

Korea University (SEJONG)

Research output: Contribution to journal › Article › peer-review

43 Citations (Scopus)

Abstract

A highly efficient selection method for enhanced enzyme enantioselectivity based on yeast surface display and fluorescence-activated cell sorting (FACS) is developed and validated. Its application to horseradish peroxidase has resulted in enzyme variants up to 2 orders of magnitude selective toward either substrate enantiomer at will. These marked improvements in enantioselectivity are demonstrated for the surface-bound and soluble enzymes and rationalized by computational docking studies.

Original language	English
Pages (from-to)	17694-17699
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	105
Issue number	46
DOIs	https://doi.org/10.1073/pnas.0809851105
Publication status	Published - 2008 Nov 18

Keywords

Directed evolution
Enzyme design
Molecular modeling
Redox enzymes
Stereoselectivity

ASJC Scopus subject areas

General

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10.1073/pnas.0809851105

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Highly L and D enantioselective variants of horseradish peroxidase discovered by an ultrahigh-throughput selection method. / Antipov, Eugene; Cho, Art E.; Wittrup, K. Dane et al.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, No. 46, 18.11.2008, p. 17694-17699.

Research output: Contribution to journal › Article › peer-review

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ER -

Highly L and D enantioselective variants of horseradish peroxidase discovered by an ultrahigh-throughput selection method

Abstract

Keywords

ASJC Scopus subject areas

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