TY - JOUR
T1 - Highly sensitive and selective detection of single-nucleotide polymorphisms using gold nanoparticle MutS enzymes and a micro cantilever resonator
AU - Park, Chanho
AU - Kang, Jimin
AU - Baek, Inchul
AU - You, Juneseok
AU - Jang, Kuewhan
AU - Na, Sungsoo
N1 - Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) under Grant Numbers NRF-2016R1A5A1010148 and NRF-2015M3A9D7031026 that are funded by the Ministry of Science and ICT, South Korea . C. Park and J. Kang contributed equally to this work.
PY - 2019/12/1
Y1 - 2019/12/1
N2 - Mutant DNAs are important markers useful for the diagnosis of human disease. Single-nucleotide polymorphisms (SNPs) represent the most common types of DNA mutations. As there is only a one base pair change in a single nucleotide between the SNP and the wild-type DNA, it is difficult to distinguish the SNPs. In this report, a highly sensitive and selective detection and discrimination of SNPs is performed using MutS, gold nanoparticles (AuNP) and a resonator. A single mismatched base exists between the SNP mutation and the probe DNA on the resonator, and MutS binds to the DNA at the location of the mismatch. As MutS is attached to AuNP (MutS-AuNP), both MutS and AuNP are adsorbed onto the resonator. The detection is based on the resonance frequency shift of the resonator following the adsorption of MutS-AuNP on the resonator. Highly sensitive detection of DNA mutations was achieved using AuNPs that act as mass amplifiers, and the obtained limit of detected was 100 fM. Additionally, our proposed method detected mutations in the presence of as little as 0.1% wild-type, and discrimination of specific mutations was also achieved. The results obtained from our proposed method suggest its potential for diagnosing cancer patients.
AB - Mutant DNAs are important markers useful for the diagnosis of human disease. Single-nucleotide polymorphisms (SNPs) represent the most common types of DNA mutations. As there is only a one base pair change in a single nucleotide between the SNP and the wild-type DNA, it is difficult to distinguish the SNPs. In this report, a highly sensitive and selective detection and discrimination of SNPs is performed using MutS, gold nanoparticles (AuNP) and a resonator. A single mismatched base exists between the SNP mutation and the probe DNA on the resonator, and MutS binds to the DNA at the location of the mismatch. As MutS is attached to AuNP (MutS-AuNP), both MutS and AuNP are adsorbed onto the resonator. The detection is based on the resonance frequency shift of the resonator following the adsorption of MutS-AuNP on the resonator. Highly sensitive detection of DNA mutations was achieved using AuNPs that act as mass amplifiers, and the obtained limit of detected was 100 fM. Additionally, our proposed method detected mutations in the presence of as little as 0.1% wild-type, and discrimination of specific mutations was also achieved. The results obtained from our proposed method suggest its potential for diagnosing cancer patients.
KW - DNA detection
KW - Gold nanoparticle (AuNP)
KW - Kirsten rat sarcoma viral oncogene homolog (KRAS)
KW - Mutant DNA
KW - Resonance frequency shift
KW - Single nucleotide polymorphism
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U2 - 10.1016/j.talanta.2019.120154
DO - 10.1016/j.talanta.2019.120154
M3 - Article
C2 - 31450442
AN - SCOPUS:85069667117
SN - 0039-9140
VL - 205
JO - Talanta
JF - Talanta
M1 - 120154
ER -