Highly sensitive micropatterned interdigitated electrodes for enhancing the concentration effect based on dielectrophoresis

Hye Jin Kim; Heeju Ahn; David S. Lee; Dongsung Park; Jae Hyun Kim; Jinsik Kim; Dae Sung Yoon; Kyo Seon Hwang

doi:10.3390/s19194152

Highly sensitive micropatterned interdigitated electrodes for enhancing the concentration effect based on dielectrophoresis

Hye Jin Kim, Heeju Ahn, David S. Lee, Dongsung Park, Jae Hyun Kim, Jinsik Kim, Dae Sung Yoon, Kyo Seon Hwang

School of Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

The concentration effect of dielectrophoresis (DEP) enables detection of biomolecules with high sensitivity. In this study, microstructures were patterned between the interdigitated microelectrodes (IMEs) to increase the concentration effect of DEP. The microstructures increased the electric field gradient (∇_E2) between the IMEs to approximately 6.61-fold higher than in the bare IMEs with a gap of 10 µm, resulting in a decreased optimal voltage to concentrate amyloid beta 42 (Aβ₄₂, from 0.8 V_pp to 0.5 V_pp) and tau-441 (from 0.9 V_pp to 0.6 V_pp) between the IMEs. Due to the concentration effect of DEP, the impedance change in the optimal condition was higher than the values in the reference condition at 2.64-fold in Aβ₄₂ detection and at 1.59-fold in tau-441 detection. This concentration effect of DEP was also verified by counting the number of gold (Au) particles which conjugated with the secondary antibody. Finally, an enhanced concentration effect in the patterned IMEs was verified by measuring the impedance change depending on the concentration of Aβ₄₂ and tau-441. Our results suggest that microstructures increase the concentration effect of DEP, leading to enhanced sensitivity of the IMEs.

Original language	English
Article number	4152
Journal	Sensors (Switzerland)
Volume	19
Issue number	19
DOIs	https://doi.org/10.3390/s19194152
Publication status	Published - 2019 Oct 1

Bibliographical note

Publisher Copyright:
© 2019 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

Amyloid beta 42
Concentration effect
Dielectrophoresis
High sensitivity
Tau-441

ASJC Scopus subject areas

Analytical Chemistry
Information Systems
Atomic and Molecular Physics, and Optics
Biochemistry
Instrumentation
Electrical and Electronic Engineering

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10.3390/s19194152

Cite this

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title = "Highly sensitive micropatterned interdigitated electrodes for enhancing the concentration effect based on dielectrophoresis",

abstract = "The concentration effect of dielectrophoresis (DEP) enables detection of biomolecules with high sensitivity. In this study, microstructures were patterned between the interdigitated microelectrodes (IMEs) to increase the concentration effect of DEP. The microstructures increased the electric field gradient (∇E2) between the IMEs to approximately 6.61-fold higher than in the bare IMEs with a gap of 10 µm, resulting in a decreased optimal voltage to concentrate amyloid beta 42 (Aβ42, from 0.8 Vpp to 0.5 Vpp) and tau-441 (from 0.9 Vpp to 0.6 Vpp) between the IMEs. Due to the concentration effect of DEP, the impedance change in the optimal condition was higher than the values in the reference condition at 2.64-fold in Aβ42 detection and at 1.59-fold in tau-441 detection. This concentration effect of DEP was also verified by counting the number of gold (Au) particles which conjugated with the secondary antibody. Finally, an enhanced concentration effect in the patterned IMEs was verified by measuring the impedance change depending on the concentration of Aβ42 and tau-441. Our results suggest that microstructures increase the concentration effect of DEP, leading to enhanced sensitivity of the IMEs.",

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AU - Kim, Jae Hyun

AU - Kim, Jinsik

AU - Yoon, Dae Sung

AU - Hwang, Kyo Seon

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Highly sensitive micropatterned interdigitated electrodes for enhancing the concentration effect based on dielectrophoresis

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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