Highly sensitive sandwich-type SPR based detection of whole H5Nx viruses using a pair of aptamers

Van Thuan Nguyen; Ho Bin Seo; Byoung Chan Kim; Sang Kyung Kim; Chang Seon Song; Man Bock Gu

doi:10.1016/j.bios.2016.06.064

Highly sensitive sandwich-type SPR based detection of whole H5Nx viruses using a pair of aptamers

Van Thuan Nguyen, Ho Bin Seo, Byoung Chan Kim, Sang Kyung Kim, Chang Seon Song, Man Bock Gu

Department of Biotechnology

Research output: Contribution to journal › Article › peer-review

95 Citations (Scopus)

Abstract

In this research, we report highly sensitive and specific sandwich-type SPR-based biosensor for the detection H5Nx whole viruses. A few of aptamers, for the first time, were successfully screened and characterized for whole avian influenza (AI) viruses, H5Nx, by using Multi-GO-SELEX method. The affinities of the aptamers developed in this study were ranged from 8×10⁴ to 1×10⁴ EID50/ml, and the aptamers IF22, IF23 were found to be specific to H5N1 and H5N8, respectively. In addition, some flexible aptamers IF20, IF15, and IF10 were found to bind to the H5N1 and H5N2, H5N1 and H5N8, or H5N1, H5N2, and H5N8, respectively. Moreover, aptamers IF10 and IF22 were found to bind H5N1 virus simultaneously and confirmed to bind the different site of the same H5N1 whole virus. Therefore, this pair of aptamers, IF10 and IF22, were successfully applied to develop the sandwich-type SPR-based biosensor assay which is rapid, accurate for the detection of AI whole virus from H5N1-infected feces samples. The minimum detectible concentration of H5N1 whole virus was found to be 200 EID50/ml with this sandwich-type detection using the aptamer pair obtained in this study. In addition, the sensitivity of this biosensor was successfully enhanced by using the signal amplification with the secondary aptamer conjugated with gold nanoparticles.

Original language	English
Pages (from-to)	293-300
Number of pages	8
Journal	Biosensors and Bioelectronics
Volume	86
DOIs	https://doi.org/10.1016/j.bios.2016.06.064
Publication status	Published - 2016 Dec 15

Bibliographical note

Funding Information:
This research was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea Government (MSIP) (No. 2016R1E1A2020541 and No. 2016R1A2B3011422 ), the Basic Core Technology Development Program for the Oceans and the Polar Regions of the National Research Foundation (NRF) funded by the Ministry of Science, ICT & Future Planning ( 2015M1A5A1037055 ) and the KIST Institutional Program (Project No. 2E00000-00-000 ). The Dr. V. T Nguyen's salary was financially supported by Korea University Grant.

Publisher Copyright:
© 2016 Elsevier B.V.

Keywords

AuNP
Circular Dichroism
Dual aptamer
SPR
Whole virus H5Nx

ASJC Scopus subject areas

Biophysics
Biotechnology
Biomedical Engineering
Electrochemistry

Access to Document

10.1016/j.bios.2016.06.064

Cite this

@article{075d3263d62e408a8b27f3ea4e013913,

title = "Highly sensitive sandwich-type SPR based detection of whole H5Nx viruses using a pair of aptamers",

abstract = "In this research, we report highly sensitive and specific sandwich-type SPR-based biosensor for the detection H5Nx whole viruses. A few of aptamers, for the first time, were successfully screened and characterized for whole avian influenza (AI) viruses, H5Nx, by using Multi-GO-SELEX method. The affinities of the aptamers developed in this study were ranged from 8×104 to 1×104 EID50/ml, and the aptamers IF22, IF23 were found to be specific to H5N1 and H5N8, respectively. In addition, some flexible aptamers IF20, IF15, and IF10 were found to bind to the H5N1 and H5N2, H5N1 and H5N8, or H5N1, H5N2, and H5N8, respectively. Moreover, aptamers IF10 and IF22 were found to bind H5N1 virus simultaneously and confirmed to bind the different site of the same H5N1 whole virus. Therefore, this pair of aptamers, IF10 and IF22, were successfully applied to develop the sandwich-type SPR-based biosensor assay which is rapid, accurate for the detection of AI whole virus from H5N1-infected feces samples. The minimum detectible concentration of H5N1 whole virus was found to be 200 EID50/ml with this sandwich-type detection using the aptamer pair obtained in this study. In addition, the sensitivity of this biosensor was successfully enhanced by using the signal amplification with the secondary aptamer conjugated with gold nanoparticles.",

keywords = "AuNP, Circular Dichroism, Dual aptamer, SPR, Whole virus H5Nx",

author = "Nguyen, {Van Thuan} and Seo, {Ho Bin} and Kim, {Byoung Chan} and Kim, {Sang Kyung} and Song, {Chang Seon} and Gu, {Man Bock}",

note = "Funding Information: This research was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea Government (MSIP) (No. 2016R1E1A2020541 and No. 2016R1A2B3011422 ), the Basic Core Technology Development Program for the Oceans and the Polar Regions of the National Research Foundation (NRF) funded by the Ministry of Science, ICT & Future Planning ( 2015M1A5A1037055 ) and the KIST Institutional Program (Project No. 2E00000-00-000 ). The Dr. V. T Nguyen's salary was financially supported by Korea University Grant. Publisher Copyright: {\textcopyright} 2016 Elsevier B.V.",

year = "2016",

month = dec,

day = "15",

doi = "10.1016/j.bios.2016.06.064",

language = "English",

volume = "86",

pages = "293--300",

journal = "Biosensors and Bioelectronics",

issn = "0956-5663",

publisher = "Elsevier Limited",

}

TY - JOUR

T1 - Highly sensitive sandwich-type SPR based detection of whole H5Nx viruses using a pair of aptamers

AU - Nguyen, Van Thuan

AU - Seo, Ho Bin

AU - Kim, Byoung Chan

AU - Kim, Sang Kyung

AU - Song, Chang Seon

AU - Gu, Man Bock

N1 - Funding Information: This research was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea Government (MSIP) (No. 2016R1E1A2020541 and No. 2016R1A2B3011422 ), the Basic Core Technology Development Program for the Oceans and the Polar Regions of the National Research Foundation (NRF) funded by the Ministry of Science, ICT & Future Planning ( 2015M1A5A1037055 ) and the KIST Institutional Program (Project No. 2E00000-00-000 ). The Dr. V. T Nguyen's salary was financially supported by Korea University Grant. Publisher Copyright: © 2016 Elsevier B.V.

PY - 2016/12/15

Y1 - 2016/12/15

N2 - In this research, we report highly sensitive and specific sandwich-type SPR-based biosensor for the detection H5Nx whole viruses. A few of aptamers, for the first time, were successfully screened and characterized for whole avian influenza (AI) viruses, H5Nx, by using Multi-GO-SELEX method. The affinities of the aptamers developed in this study were ranged from 8×104 to 1×104 EID50/ml, and the aptamers IF22, IF23 were found to be specific to H5N1 and H5N8, respectively. In addition, some flexible aptamers IF20, IF15, and IF10 were found to bind to the H5N1 and H5N2, H5N1 and H5N8, or H5N1, H5N2, and H5N8, respectively. Moreover, aptamers IF10 and IF22 were found to bind H5N1 virus simultaneously and confirmed to bind the different site of the same H5N1 whole virus. Therefore, this pair of aptamers, IF10 and IF22, were successfully applied to develop the sandwich-type SPR-based biosensor assay which is rapid, accurate for the detection of AI whole virus from H5N1-infected feces samples. The minimum detectible concentration of H5N1 whole virus was found to be 200 EID50/ml with this sandwich-type detection using the aptamer pair obtained in this study. In addition, the sensitivity of this biosensor was successfully enhanced by using the signal amplification with the secondary aptamer conjugated with gold nanoparticles.

AB - In this research, we report highly sensitive and specific sandwich-type SPR-based biosensor for the detection H5Nx whole viruses. A few of aptamers, for the first time, were successfully screened and characterized for whole avian influenza (AI) viruses, H5Nx, by using Multi-GO-SELEX method. The affinities of the aptamers developed in this study were ranged from 8×104 to 1×104 EID50/ml, and the aptamers IF22, IF23 were found to be specific to H5N1 and H5N8, respectively. In addition, some flexible aptamers IF20, IF15, and IF10 were found to bind to the H5N1 and H5N2, H5N1 and H5N8, or H5N1, H5N2, and H5N8, respectively. Moreover, aptamers IF10 and IF22 were found to bind H5N1 virus simultaneously and confirmed to bind the different site of the same H5N1 whole virus. Therefore, this pair of aptamers, IF10 and IF22, were successfully applied to develop the sandwich-type SPR-based biosensor assay which is rapid, accurate for the detection of AI whole virus from H5N1-infected feces samples. The minimum detectible concentration of H5N1 whole virus was found to be 200 EID50/ml with this sandwich-type detection using the aptamer pair obtained in this study. In addition, the sensitivity of this biosensor was successfully enhanced by using the signal amplification with the secondary aptamer conjugated with gold nanoparticles.

KW - AuNP

KW - Circular Dichroism

KW - Dual aptamer

KW - SPR

KW - Whole virus H5Nx

UR - http://www.scopus.com/inward/record.url?scp=84978967803&partnerID=8YFLogxK

U2 - 10.1016/j.bios.2016.06.064

DO - 10.1016/j.bios.2016.06.064

M3 - Article

C2 - 27387259

AN - SCOPUS:84978967803

SN - 0956-5663

VL - 86

SP - 293

EP - 300

JO - Biosensors and Bioelectronics

JF - Biosensors and Bioelectronics

ER -

Highly sensitive sandwich-type SPR based detection of whole H5Nx viruses using a pair of aptamers

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this